Comparison of two systems of tonoplast purification from tobacco cells of suspension culture BY-2

Abstract

The tonoplast is an intracellular membrane, important for numerous functions of the vacuoles in plant cells. Transport, signaling, enzymatic activity and other processes of the tonoplast are the focus of a wide spectrum of studies. Multiple advanced analyses demand highly purified vacuoles or vesicles of the tonoplast membrane fraction. Since 1960 several approaches have been developed for such purification, but new goals and new model objects require adjustment of already existing techniques. The presented investigation aimed to compare two methods of tonoplast membrane fraction purification from tobacco suspension cell culture BY-2 (Nicotiana tabacum L., cv Bright Yellow) based on sucrose and sucrose/sorbitol gradients. The functional activity of obtained purified tonoplast fractions was measured by the hydrolytic activity of vacuolar H+-ATPase and H+-PPase.

Proteome and Lipidome of Plant Cell Membranes during Development

“…It is based on using of two immiscible solutions of polyethylene glycol and dextran; their concentrations are species-and tissue-specific. Some approaches were proposed for tonoplast isolation [27][28][29], although they are not generally accepted and require further improvement so far. After preparing a material containing a certain purified membrane fraction or that is enriched with it, a scientist faces several more important problems.…”