Comparison of two techniques used to collect normal and motile sperm

Szerman-Joly, E.; Sauvalle, A.; Izard, Juan Francisco Martín; Drosdowsky, M. A.

doi:10.1111/j.1365-2605.1989.tb01305.x

Cited by 4 publications

(2 citation statements)

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“…As the MST is a simple and economical procedure, it can be used as a screening test before IVF-ET attempts if the results can be correlated to fertilizing capacity. The improvements in sperm characteristics after MST in the present study ( Table 2 ) are similar to those reported by others (Tea et al, 1983;Lucena et al, 1989;Szerman-Joly et al, 1989). Motility was improved by c.9796, and the percentage of normal forms by c. 19%.…”

Section: Discussionsupporting

confidence: 91%

“…The migration-sedimentation technique (MST) was first suggested by Tea et al ( 1983) for the separation of spermatozoa based upon the spontaneous migration and sedimentation of motile cells in a special device without the use of centrifugation. Studies which have used MST reported significant improvement in sperm motility of about 60% and morphology of about 47% of the separated sperm (Tea et al, 1983;Lucena et al, 1989;Szerman-Joly et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Migration sedimentation technique as a predictive test for the fertilizing capacity of spermatozoa in an in‐vitro fertilization programme

Hauser

Homonnai²,

Paz³

et al. 1992

Int J of Andrology

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The migration-sedimentation technique (MST) has been proposed as a means of separating high quality motile spermatozoa. The present study was conducted in order to evaluate whether sperm performance following separation by MST predicts their fertilizing capacity in an in-vitro fertilization (IVF) programme. Ninety semen specimens were analysed for use in an IVF-embryo transfer (ET) programme. Each specimens was divided into two parts: one was processed in the IVF programme and was used after sperm swim-up separation for insemination of human ova. The other aliquot (0.2 ml) was separated by MST, and the sperm then characterized by their concentration, motility, degree of motility and morphology. Sperm characteristics after separation by MST were then correlated with the results of the IVF-fertilization rates. In 79 of 90 IVF-ET cycles, at least one oocyte was fertilized. All post-MST sperm characteristics were significantly higher in cycles with fertilizations compared to IVF cycles without fertilization. A larger percentage of the total motile spermatozoa were recovered after MST in semen specimens with fertilization, compared to semen specimens without fertilization (39.9 +/- 3.6 and 20.6 +/- 6.6%, respectively; P < 0.05). This value was correlated with the percentage of fertilized oocytes (r = 0.24; P < 0.02). More IVF cycles with fertilizations were recorded in cases in which the recovery of motile sperm was > 25% (P < 0.005), or when more than 1.5 x 10(6) motile spermatozoa were recovered after MST (P < 0.0001). As sperm characteristics after MST correlated significantly with their fertilizing capacity, the MST test could be used in evaluation of the fertilizing capacity of spermatozoa.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Migration sedimentation technique as a predictive test for the fertilizing capacity of spermatozoa in an in‐vitro fertilization programme

Hauser

Homonnai²,

Paz³

et al. 1992

Int J of Andrology

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Separation of sperm cells by sedimentation technique is not suitable for in vitro fertilization purposes

et al. 2009

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Two methods of sperm preparation for in uitro fertilization were compared: the swimup technique vs. the migration-sedimentation technique. The study comprised fresh semen samples obtained from 25 couples treated in the In Ztro Fertilization Unit. Oocytes aspirated in a single cycle were divided into two groups, each inseminated by sperm prepared by one of these techniques. Motility, degree of motility, and normal morphology were improved by both methods. The improvement was greater when the migrationsedimentation technique was applied. However, fertilization rate was significantly higher after the swim-up technique. In order to clarify this contradiction, an additional group of 26 semen samples was divided and then prepared by the swim-up or migration-sedimentation techniques. Sperm quality was examined up to 72 h after separation.Compared with the swim-up technique, sperm characteristics were better after separation by the migration-sedimentation technique. However, this difference abated after 24 h. The better results of the swim-up technique in the 'survival experiment' may explain its improved performance in in uitro fertilization, despite lower separation capacity. Thus, the migration-sedimentation technique is not recommended for sperm preparation in in vitro fertilization.

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Preparation of Human Sperm for Assisted Conception: A Comparative Study

Gabriel

Vawda

1993

Archives of Andrology

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An ideal sperm preparation technique for assisted conception requires the capacity of accumulating in a relatively small volume the largest number of morphologically normal, viable sperm with good motility. Additionally, this extract of the ejaculate must be free of seminal plasma, leukocytes, bacteria, and other debris. In a comparison of the migration-sedimentation, SpermPrep, and swim-up techniques for sperm preparation, MS proved best at improving sperm motility and morphology in samples from both fertile and subfertile patients. In the fertile group, motility improved by 32% and morphology by 39%, while the subfertile group demonstrated improvements of 49% and 29%, respectively. SpermPrep produced the highest sperm yield after only 15 min of processing. The yield was in the region of 37% for the fertile group and 17% for the subfertile group. All samples demonstrated an improvement in the grade of progressive sperm motility and a decrease in debris content after processing with all three methods.

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Comparison of two techniques used to collect normal and motile sperm

Cited by 4 publications

References 13 publications

Migration sedimentation technique as a predictive test for the fertilizing capacity of spermatozoa in an in‐vitro fertilization programme

Migration sedimentation technique as a predictive test for the fertilizing capacity of spermatozoa in an in‐vitro fertilization programme

Separation of sperm cells by sedimentation technique is not suitable for in vitro fertilization purposes

Preparation of Human Sperm for Assisted Conception: A Comparative Study

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