Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis

Evans, R.T.; Taylor‐Robinson, David

doi:10.1128/jcm.10.2.198-201.1979

Cited by 63 publications

(19 citation statements)

References 17 publications

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“…These specimens were kept at 4°C for a maximum of three hours before being stored in liquid nitrogen. They were thawed in a 37°C waterbath and a 1.0 ml amount was tested for chlamydiae by centrifugation on to cycloheximide-treated Mc-Coy cells as described previously (Evans and Taylor-Robinson, 1979). In addition, 0 .…”

Section: Laboratory Investigationsmentioning

confidence: 99%

The Effect of Genital Mycoplasmas on Human Fetal Growth

Ross¹,

Furr²,

Taylor‐Robinson³

et al. 1981

BJOG

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The relation between maternal genital colonization by mycoplasmas and fetal growth was examined in a study of 195 women. Swabs were taken from the endocervix on three occasions during pregnancy and once post partum. Ureaplasma urealyticum organisms (ureaplasmas) were recovered from 42.7 per cent of Caucasian women and from 34.6 per cent of Asian women at their first antenatal visit. These isolation rates remained similar throughout pregnancy, although there was a decrease in isolation after delivery. Mycoplasma hominis was recovered from 6 5 per cent of Caucasians and from 1 1 5 per cent of Asians at their first antenatal visit and these rates remained fairly constant during pregnancy and after delivery. Caucasian women colonized by ureaplasmas had a longer mean length of gestation (p < 0.025) than non-colonized women. Furthermore, the colonized women gave birth to infants who had a statistically significant greater mean birth weight and a greater mean birth weight-for-dates than those of the non-colonized Caucasians. There was no correlation between gestational length, birth weight, or birth weightfor-dates and genital colonization of Asian mothers by ureaplasmas or M . hominis. It is clear that ureaplasmas are not associated with low birth weight in our population.

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Section: Laboratory Investigationsmentioning

confidence: 99%

The Effect of Genital Mycoplasmas on Human Fetal Growth

Ross¹,

Furr²,

Taylor‐Robinson³

et al. 1981

BJOG

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“…MoPn was isolated by inoculation of McCoy cell cultures by using centrifugation essentially as described by Evans and Taylor-Robinson (10). The McCoy cells were initially grown on cover slips in shell vials in Eagle minimum essential medium and at later times were grown on cover slips in 24-well culture dishes (Nunc, Roskilde, Denmark).…”

mentioning

confidence: 99%

Resolution of chlamydial genital infection in B-cell-deficient mice and immunity to reinfection

1988

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The purpose of this investigation was to determine the relative roles of the humoral and cell-mediated immune responses in the resolution of chlamydial genital infection of mice and resistance to reinfection. To this end, female BALB/c mice were rendered B cell deficient by treatment with heterologous anti-immunoglobulin M (IgM) serum from birth. Controls were similarly treated with either normal serum or phosphate-buffered saline. Before inclusion in each experiment, aiti-IgM-treated mice were screened for the absence of IgM in serum and for the presence of cell-mediated immune responses. In addition, ppleen cells from anti-IgM-treated mice responded to concanavalin A and phytohemagglutinin but not to lipopolysaccharide. By these criteria, mice were designated B cell deficient. B-cell-deficient mice and controls were inoculated intravaginally with a suspension of mouse pnecvumonitis agent (MoPn), a Chiamydia trachomatis biovar. All B-cell-deficient mice resolved the infection. Additionally, no significant difference was seen in the course of the infection in B-cell-deficient mice when compared with controls. In contrast to control mice, B-cell-deficient mice displayed no detectable antibody responses to MoPn in serum or in genital secretions. However, both B-cell-deficient mice and controls developed delayed-type hypersensitivity and T-cell proliferative responses to MoPn. When challenged 53 days after primary infection, no significant difference was seen in the resistance of B-cell-deficient mice to reinfection when compared with that of the controls. These data indicate that cell-mediated immune mechanisms play an important role in the resolution of and resistance to chlamydial genital infection in this model.

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“…This was regarded as a 10-fold dilution and was diluted serially so that the numbers of organisms, expressed as colour-changing units (ccu), could be estimated (Taylor-Robinson and Purcell, 1966). Specimens were examined also for genital strains of Chlamydia trachornatis (chlamydiae) using cycloheximide-treated McCoy cells (Evans and Taylor-Robinson, 1979).…”

Section: Microbiological Methodsmentioning

confidence: 99%

Chronic Cystitis and Urethritis Associated with Ureaplasmal and Mycoplasmal Infection in Primary Hypogammaglobulinaemia

Webster

Taylor‐Robinson

Furr

et al. 1982

British Journal of Urology

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Six of 58 patients with primary hypogammaglobulinaemia developed chronic urethritis and/or cystitis. We have some evidence that this complication may be caused by infection with strains Rof Ureaplasma urealyticum. This is important because ureaplasmas are usually resistant to most antibiotics routinely used to treat lower urinary tract infections. It appears that hypogammaglobulinaemic patients develop less localised and more severe ureaplasmal infections than immunocompetent subjects, which indicates that antibodies are important in controlling the growth of these organisms in the bladder and urethra.

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Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis

Cited by 63 publications

References 17 publications

The Effect of Genital Mycoplasmas on Human Fetal Growth

The Effect of Genital Mycoplasmas on Human Fetal Growth

Resolution of chlamydial genital infection in B-cell-deficient mice and immunity to reinfection

Chronic Cystitis and Urethritis Associated with Ureaplasmal and Mycoplasmal Infection in Primary Hypogammaglobulinaemia

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