Comparison of VEGF-A levels in women with threatened abortion, early pregnancy loss and uncomplicated healthy pregnancies

Ozden Tokalioglu, Eda; Turgut, Ezgi; Gulen Yildiz, Esra; Ozturk Agaoglu, Merve; Biriken, Derya; Tanacan, Atakan; Yazihan, Nuray; Sahin, Dilek

doi:10.1016/j.cyto.2023.156343

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…Although there are many angiogenic factors contributing to the fetal development process, VEGF is the most prominent positive regulator of this process and it has a key role in the endometrium during embryo implantation (Wiltbank et al, 2016) are significantly increased in diseases such as pre-eclampsia (PE) (Kupferminc et al, 1997;Sharkey et al, 1996), recurrent spontaneous abortion (Pang et al, 2013) and early pregnancy loss (Col-Madendag et al, 2014;Tokalioglu et al, 2023). On the contrary, others suggest that reduced levels of VEGF play an important role in PE (Charkiewicz et al, 2018) and miscarriages (Baliyan et al, 2018) in women.…”

Section: Discussionmentioning

confidence: 99%

Early prognostic markers to predict unsuccessful pregnancy in dairy cattle

Yokus,

Takci,

Ercan

et al. 2024

Reprod Domestic Animals

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This study aimed to investigate maternal serum levels of some angiogenic factors and certain proteins in dairy cattle for (1) early prediction of unsuccessful fertilization and (2) early detection of possible pregnancy failures (early EM) after positive insemination Serum samples were collected from the same cattle at three distinct time points: 30 days before artificial insemination (B‐AI), on the day of artificial insemination (AI), and 30 days after artificial insemination (A‐AI). As a result of the pregnancy examination, the cows were divided into two main groups according to whether they were pregnant. The results showed that leucyl/cystinyl aminopeptidase (LNPEP) concentration was significantly decreased B‐AI and Secreted frizzled‐related proteins (SFRP‐3), Vascular Endothelial Growth Factor (VEGF) and LNPEP levels were significantly decreased on day of AI, while PRL level was increased, and these data have prognostic significance as early indicator of the risk of potentially failed pregnancy. Additionally, a significant decrease in LNPEP, SFRP3, and VEGF levels, along with an increase in PRL levels was also observed in A‐AI. These results suggest that these biomarkers can be used as a screening test to monitor the course of pregnancy. There were no significant differences in serum levels of Insulin‐Like Growth Factor 2 (IGF‐2), Tissue inhibitors of metalloproteinases (TIMP‐1), angiopoietin (ANG), Endoglin (ENG), Fibroblast growth factor (FGF), Inhibine‐A (INH‐A) and Transforming growth factors‐β1 (TGF‐β1) between the evaluated periods neither unsuccessful nor the successful pregnancy groups. This is the first study reporting that the maternal serum levels of LNPEP, SFRP3, VEGF, and PRL have important roles in pregnancy success and may indicate whether insemination outcome will be successful B‐AI and predict the risk of unsuccessful pregnancy after AI in dairy cattle. The increase in such studies will allow the development of more specific, practical, and applicable markers.

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Section: Discussionmentioning

confidence: 99%

Early prognostic markers to predict unsuccessful pregnancy in dairy cattle

Yokus,

Takci,

Ercan

et al. 2024

Reprod Domestic Animals

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Effects of OPN and LIF expression in decidual and villous tissues on unexplained recurrent spontaneous abortion in the first trimester of pregnancy

Wen,

Chen,

et al. 2024

Preprint

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Objective Recurrent spontaneous abortion (RSA) is defined as a condition in which women suffer from two or more continuous spontaneous miscarriages. However, the etiology of a large proportion of women with RSA remains unknown. The study was conducted to investigate the effects of expression of osteopontin (OPN) and leukemic inhibitory factor (LIF) expression in decidual and villous tissues on unexplained RSA in the first trimester of pregnancy. Methods Twenty women with unexplained RSA in the first trimester of pregnancy were selected as study group, as well as 20 women with normal induced abortion in the first trimester of pregnancy were selected as control group. The decidual and villous tissues of participants in the two groups were collected. The expressions of OPN and LIF were detected by immunohistochemistry (IHC), immunofluorescence, western blotting and real-time polymerase chain reaction. The correlation between the expressions of OPN and LIF was analyzed. Results The OPN and LIF were mainly expressed in uterine epithelial cells and decidual cells, as well as pregnancy trophoblastic cells in villous tissues. The positive rates of OPN and LIF expression in decidual and villous tissues of the study group were significantly decreased, and the IHC scores were significantly lower compared with control group (P < 0.05). In the decidual tissues, the OPN and LIF were mainly co-located in uterine epithelial cells and decidual cells. While in the villous tissues, OPN and LIF were mainly co-located in gestational trophoblastocytes. The fluorescence intensities of OPN and LIF in decidual and villous tissues of the study group were significantly lower than that of the control group (P < 0.05). In addition, the protein and mRNA expression levels of OPN and LIF in decidual and villous tissues in study group were also significantly decreased compared with control group (P < 0.05). The protein and mRNA expression levels of OPN and LIF in decidual and villous tissues of the two groups were significantly positively correlated (P < 0.01). Conclusion The expressions of OPN and LIF in decidual and villous tissues of patients with unexplained RSA in the first trimester of pregnancy were significantly decreased, which may lead to reduced endometrial receptivity, and then cause RSA.

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Comparison of VEGF-A levels in women with threatened abortion, early pregnancy loss and uncomplicated healthy pregnancies

Cited by 2 publications

References 34 publications

Early prognostic markers to predict unsuccessful pregnancy in dairy cattle

Early prognostic markers to predict unsuccessful pregnancy in dairy cattle

Effects of OPN and LIF expression in decidual and villous tissues on unexplained recurrent spontaneous abortion in the first trimester of pregnancy

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