Comparison of viability of oral epithelial cells stored by different freezing methods

Abstract

This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting, WST-1, and clonogenic capacity values were measured and compared.1. The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 2. The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 3. The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). [J Kor Acad Cons Dent 34(5):491-499, 2009]

Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method

“…This study stored rat teeth under 0 o C/2 MPa condition, which maintained the PDL cell higher in previous studies. Many studies presented that keeping the cell under pressure option maintained its viability higher than using organs such as tissues or embryos [18][19][20][21] . However, among the studies using teeth, the cell viability reduced rapidly over 3 MPa 12,19) .…”

“…Many studies presented that keeping the cell under pressure option maintained its viability higher than using organs such as tissues or embryos [18][19][20][21] . However, among the studies using teeth, the cell viability reduced rapidly over 3 MPa 12,19) . Therefore, this study selected the 0 o C/2 MPa condition which represented consistently high cell viability.…”