Comparison of Vibrio cholerae O1 isolates by polymerase chain reaction fingerprinting and ribotyping

Antimicrobial susceptibility and molecular characterization of Vibrio cholerae from cholera outbreaks in Chennai

“…Moreover, the rep-PCR methods have been reported to be more discriminatory and have the potential to substitute the cumbersome ribotyping [6]. The VC1 primer derived from the ERIC related sequence in V. cholerae was used in rep-PCR fi ngerprinting.…”

“…Different combinations of primers derived from repetitive DNA sequences have been successfully used in molecular typing of V. cholerae strains elsewhere. PCR DNA fi ngerprinting using the primers based on these repeat sequences are easy to perform and can very well aid in bringing out the relatedness or differences among the V. cholerae isolates [6].…”

Random Amplified Polymorphic Dna-PCR Typing of Vibrio parahaemolyticus Isolated from Local Cockles (Anadara granosa)

“…In order to obtain a sufficient number of polymorphic bands that will permit reliable comparison, the three different 10-mer primers were used in amplification reactions. Previously, Shangkuan et al [12] demonstrated the importance of using a minimum of three different primers to discriminate different isolates of toxigenic V. cholerae. The results of using three primers in this study indicate that RAPD provides a high degree of discrimination between V. parahaemolyticus strains, with amplified products ranging from 0.25 to 10.0 kb.…”

“…A rapid typing method based on the random amplification of polymorphic DNA segments (RAPD-PCR) has been described [10,11] . This method has been successfully used in the characterization of several organisms including V. parahaemolyticus and other vibrios [1,[12][13][14] . In this study, a library of 10 primers (50% G+C) was examined for suitability.…”

Diversidade de bactérias em amostras de água do mar no canal de São Sebastião

“…A extensão da diversidade microbiana está ainda em análise (CURTIS et al, 2002) e técnicas moleculares têm permitido a detecção de um número crescente de novos microrganismos (WARD et al, 1990;PACE, 1997;HUGENHOLTZ et al, 1998b;DeLONG, 2001 (SHANGKUAN et al, 1997;MARSHALL et al, 1999;WONG e LIN, 2001 BRUIJN, 1997;VERSALOVIC et al, 1994;WOODS et al, 1993 A diversidade bacteriana foi estudada em vários ambientes baseando-se nos genes 16S rDNA dos seus componentes, revelando a grande diversidade que deixa de ser explorada quando apenas métodos baseados em cultivo são aplicados (HEAD et al, 1998). Utilizando técnicas independentes de cultivo baseadas em seqüências do gene 16S rDNA foi descrita a comunidade bacteriana do oceano, sendo estimado a presença de valores próximos a 21.000 seqüências diferentes compondo esta comunidade (SOGIN et al, 2006).…”