Comparison of virus detection by EIA and cell culture

Abstract

Experientia 42 (1986), Birkhfiuser Verlag, CH-4010 Basel/Switzerland 101 BCB-blood culture system (Roche) detects Brucella bacteremia in less than 8 days R. Auckenthaler, B. Hirschel and S. Shiro-Lente H6pital Cantonal Universitaire, CH-121t Gen~ve From July 1982 to December 1984 all blood culture bottles from patients with fever of unknown origin, suspected endocarditis, or brucellosis were routinely incubated at 35 ~ for 14 days using BCB (Roche) system. Aerobic bottles (AER) contained columbia broth and anaerobic bottles (ANAER) thioglycolate broth. Subcultures were performed using BCB slides for AER twice a day during the first 48 h and daily thereafter. ANAER was subcultured only if AER was positive or if broth became turbid. 63/71 submitted AER originated from 16 patients with at least two cultures positive for Brucella spp. with a mean detection time of 6.2 days (range 4-10 d). 8/71 submitted AER (5/7 patients with previous antibiotics) were negative. At least one positive culture was detected within 8 days in all cases. 13/39 submitted ANAER became positive ouly after venting and subculturing, with a mean detection time of 10.2 days (range 7-17 days). 98.4 % of positive cultures were detectable within 8 days. In the BCB Roche system incubation more than 10 days does not seem to be necessary for detection ofBrucella spp., if vented or subcultured properly.alvei (3), S. marcescens (6), P. mirabilis (9), P. vulgaris (7), P. rettgeri (6), M. morganii (8), C. freundii (15), C. diversus (13), and Salmonella sp. (1 I). Cohas-Bact ~ agreed with API 20E on 137 identifications (99%). However 16.7% of these (23/138) required additional tests as well as all the Shigella tested (t0). One single isolate (S. marcescens) was not identified by Cobas-Bact | When restricted to the most common strains of Enterobacteriaceae, the Cobas-Bact ~ instrument appears promising for their rapid identification though a sizeable portion of the strains still requires additional tests.On the surface of conventional membrane filters with a pore size of 0.45 gm a grid of hydrophobic material is printed on so that the filtration area is divided into 1800 individual growth compartments. The hydrophobic property of the grid lines determines the positions and limits the lateral growth of microbial colonies and produces a miniaturized most probable number system equivalent to a large number of tubes inoculated from a single dilution. HGMF provides a counting range of more than 3 log 10 cycles on a single membrane filter. Gas-liquid chromatography as a complement in the research of Clostridium difficile in stools of clinically relevant patients S. Taillens, J. Barman and A. Gallusser Institut de Microbiologie, CHUV, CH IOI] LausanneThe research of C. difficile in stools of patients with antibiotic associated diarrhea or with pseudomembranous colitis is of routine in many laboratories. This research is mainly done using a selective agar with cycloserine and cefoxitin (CCFA). When the laboratory has an available source of cell cultures (human l...