Comparison of Whey Proteome and Glycoproteome in Bovine Colostrum and Mature Milk

Zhang, Wenyuan; Lü, Jing; Chen, Baorong; Gao, Peng; Song, Bo; Zhang, Shuwen; Pang, Xiaoyang; Hettinga, Kasper; Lv, Jiaping

doi:10.1021/acs.jafc.3c02085

Cited by 9 publications

(4 citation statements)

References 45 publications

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“…The protein content of the colostrum produced initially following birth of a calf contains roughly 70–80% immunoglobulins, which rapidly falls off within days to as low as 1% in the milk. Furthermore, the content of β-lactoglobulin (β-LG) and α-lactalbumin (α-LA) vary widely in colostrum, with ranges of 8 to 30 mg/mL and 8 to 14 mg/mL, respectively [ 3 , 4 ]. Furthermore, the protein concentration continues to vary as lactation proceeds.…”

Section: Introductionmentioning

confidence: 99%

SVR Chemometrics to Quantify β-Lactoglobulin and α-Lactalbumin in Milk Using MIR

Babatunde,

Collins,

Lukman

et al. 2024

Foods

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Protein content variation in milk can impact the quality and consistency of dairy products, necessitating access to in-line real time monitoring. Here, we present a chemometric approach for the qualitative and quantitative monitoring of β-lactoglobulin and α-lactalbumin, using mid-infrared spectroscopy (MIR). In this study, we employed Hotelling T2 and Q-residual for outlier detection, automated preprocessing using nippy, conducted wavenumber selection with genetic algorithms, and evaluated four chemometric models, including partial least squares, support vector regression (SVR), ridge, and logistic regression to accurately predict the concentrations of β-lactoglobulin and α-lactalbumin in milk. For the quantitative analysis of these two whey proteins, SVR performed the best to interpret protein concentration from 197 MIR spectra originating from 42 Cornell University samples of preserved pasteurized modified milk. The R2 values obtained for β-lactoglobulin and α-lactalbumin using leave one out cross-validation (LOOCV) are 92.8% and 92.7%, respectively, which is the highest correlation reported to date. Our approach introduced a combination of preprocessing automation, genetic algorithm-based wavenumber selection, and used Optuna to optimize the framework for tuning hyperparameters of the chemometric models, resulting in the best chemometric analysis of MIR data to quantitate β-lactoglobulin and α-lactalbumin to date.

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Section: Introductionmentioning

confidence: 99%

SVR Chemometrics to Quantify β-Lactoglobulin and α-Lactalbumin in Milk Using MIR

Babatunde,

Collins,

Lukman

et al. 2024

Foods

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“…WPI is a popular nutritional supplement due to its complete amino acid profile, abundance of branched-chain amino acids, and high digestibility [1]. Bovine milk-derived WPI contains hundreds of proteins, including β-lactoglobulin, α-lactalbumin, immunoglobulins, serum albumin, lactoferrin, and lactoperoxidase [2]. WPI also contains the peptide glycomacropeptide (GMP) [1].…”

Section: Introductionmentioning

confidence: 99%

Macrophage-Immunomodulatory Actions of Bovine Whey Protein Isolate, Glycomacropeptide, and Their In Vitro and In Vivo Digests

Olsen,

Liang,

Dallas

2023

Nutrients

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Whey protein isolate (WPI) consists of an array of proteins and peptides obtained as a byproduct of the cheesemaking process. Research suggests that WPI, along with its peptides such as glycomacropeptide (GMP), possesses immunomodulatory properties. These properties hold potential for alleviating the adverse effects of inflammatory conditions such as inflammatory bowel disease. Although promising, the immunoregulatory properties of the digested forms of WPI and GMP—those most likely to interact with the gut immune system—remain under-investigated. To address this knowledge gap, the current study examined the effects of in vitro-digested WPI and GMP, in vivo-digested WPI, and undigested WPI and GMP on the secretion of pro-inflammatory cytokines (TNF-α and IL-1β) in lipopolysaccharide-stimulated macrophage-like cells. Our results indicate that digested WPI and GMP reduced the expression of TNF-α and IL-1β, two pro-inflammatory cytokines. Whole WPI had no effect on TNF-α but reduced IL-1β levels. In contrast, in vivo-digested WPI reduced TNF-α but increased IL-1β. Undigested GMP, on the other hand, increased the secretion of both cytokines. These results demonstrate that digestion greatly modifies the effects of WPI and GMP on macrophages and suggest that digested WPI and GMP could help mitigate gastrointestinal inflammation. Further clinical studies are necessary to determine the biological relevance of WPI and GMP digestion products within the gut and their capacity to influence gut inflammation.

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“…Chronic inflammation is mediated by cytokines, small proteins that promote or regulate the state of inflammation. High levels of the cytokines interleukin (IL)-1, IL-8, IL-10, IL-13, TNF-α and especially IL-6 are present in adults with inflammaging [ 4 , 5 , 6 ] and are linked to the onset of illnesses such as type 2 diabetes mellitus, osteoporosis, Alzheimer’s disease, rheumatoid arthritis and coronary heart disease [ 7 , 8 ]. Potentially, changes in diet could alter inflammatory protein profiles, which could lessen inflammation-associated disease states.…”

Section: Introductionmentioning

confidence: 99%

“…WPI, derived from cheesemaking, is composed of several subtypes of proteins and peptides. Whey protein is made up of hundreds of unique proteins [ 7 ]. Among these, the whey proteins lactoferrin and α-lactalbumin and the κ-casein-derived peptide glycomacropeptide (GMP) have various bioactivities, including immunomodulatory properties [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Whey Protein Supplementation on Inflammatory Marker Concentrations in Older Adults

Adler,

Olsen,

Rackerby

et al. 2023

Nutrients

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Although whey protein isolate (WPI) has been shown to be immunomodulatory, its ability to modulate production of a broad array of inflammatory markers has not previously been investigated in healthy adults. We investigated the effects of daily supplementation with 35 g of WPI for 3 weeks on inflammatory marker concentrations in the blood serum and feces of 14 older adult subjects (mean age: 59). Serum was analyzed using a multiplex assay to quantify the cytokines IFN-γ, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-17A and TNF-α. Fecal samples were analyzed using an ELISA for the inflammatory markers calprotectin and lactoferrin. Our results yielded high inter-subject variability and a significant proportion of cytokine concentrations that were below our method’s limit of quantification. We observed decreases in serum IL-12p70 in the washout phase compared with baseline, as well as the washout stage for fecal lactoferrin relative to the intervention stage. Serum IL-13 was also significantly reduced during the intervention and washout stages. Our data suggest that whey protein supplementation did not significantly alter most inflammatory markers measured but can alter concentrations of some inflammatory markers in healthy older adults. However, our study power of 35% suggests the number of participants was too low to draw strong conclusions from our data.

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Comparison of Whey Proteome and Glycoproteome in Bovine Colostrum and Mature Milk

Cited by 9 publications

References 45 publications

SVR Chemometrics to Quantify β-Lactoglobulin and α-Lactalbumin in Milk Using MIR

SVR Chemometrics to Quantify β-Lactoglobulin and α-Lactalbumin in Milk Using MIR

Macrophage-Immunomodulatory Actions of Bovine Whey Protein Isolate, Glycomacropeptide, and Their In Vitro and In Vivo Digests

Effects of Whey Protein Supplementation on Inflammatory Marker Concentrations in Older Adults

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