2023
DOI: 10.1186/s40635-023-00556-w
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Comparison of whole blood cytokine immunoassays for rapid, functional immune phenotyping in critically ill patients with sepsis

Anthony S. Bonavia,
Abigail Samuelsen,
Menglu Liang
et al.

Abstract: Background Sepsis is characterized by highly heterogeneous immune responses associated with a spectrum of disease severity. Methods that rapidly and sensitively profile these immune responses can potentially personalize immune-adjuvant therapies for sepsis. We hypothesized that the ELLA microfluidic approach to measure cytokine production from the whole blood of septic and critically ill patients would deliver faster, more precise results than the existing optic-driven ELISpot quantification. W… Show more

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Cited by 5 publications
(8 citation statements)
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“…This study is not the first to demonstrate reduced IFN-γ ELISpot expression in septic patients, especially in those with adverse clinical outcomes (20,21,48,49). However, in contrast to these previous studies, we used diluted whole blood in our ELISpot assay instead of isolated PBMCs and observed increased IFN-γ production in both unstimulated and stimulated whole blood from critically ill patients.…”
Section: Discussionmentioning
confidence: 75%
“…This study is not the first to demonstrate reduced IFN-γ ELISpot expression in septic patients, especially in those with adverse clinical outcomes (20,21,48,49). However, in contrast to these previous studies, we used diluted whole blood in our ELISpot assay instead of isolated PBMCs and observed increased IFN-γ production in both unstimulated and stimulated whole blood from critically ill patients.…”
Section: Discussionmentioning
confidence: 75%
“…Our assay builds on the foundational work of Hotchkiss et al ( 9 ), Barrios et al ( 16 ), and Venet et al ( 17 ) by incorporating IFNγ to evaluate immune functionality ( 26 , 27 ). Crucially, by normalizing IFNγ levels to cell counts, our method confirms the presence of both quantitative and qualitative lymphocyte defects.…”
Section: Discussionmentioning
confidence: 99%
“…A further study limitation involves the method of normalizing cytokine concentrations to specific leukocyte subsets, guided by prior research identifying the primary populations that produce these cytokines (8,16,(27)(28)(29)(30)40). While normalizing IFNγ to lymphocyte count is a standard approach, normalizing IL-6 to a specific cell population presents challenges due to its pleiotropic nature.…”
Section: Discussionmentioning
confidence: 99%
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