Comparison of whole-cell protein electrophoretic profiles of <i>Haemophilus influenzae</i>: implementation of a microcomputer mainframe linked system and description of a new similarity coefficient

Albritton, William L.; Chen, X. P.; Khanna, Vinay K.

doi:10.1139/m88-199

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…hyacinthi strains. A similar optimization has been applied by other investigators (Albritton et al, 1988;Costas et al, 1989;Jackman et al, 1983). The overall reproducibility of the electrophoresis technique averaged r = 0-94.…”

Section: Sds-page Of Whole-cell Proteinsmentioning

confidence: 94%

Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins

Vauterin

Swings

Kersters

1991

Journal of General Microbiology

100

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A numerical analysis was performed on SDS-PAGE protein patterns of 307 Xanthomonas strains comprising all species and 27 X. campestris pathovars. The electrophoretic groupings corresponded in some, but not all cases with the existing pathovars. Six pathovars constituted distinct entities, comprising all strains investigated. These included X. campestris pv. campestris, X. campestris pv. graminis, X. campestris pv. hyacinthi, X. campestris pv. pelargonii, X. campestris pv. pruni and X. campestris pv. theicola. A great number of pathovars consisted of a homogeneous group from which only one or a few strains were aberrant. In two cases (X. campestris pv. ricini and X. campestris pv. vitians) even the pathovar reference strain was aberrant. Six pathovars from members of the plant family Fabaceae could not be differentiated from one another: X. campestris pv. phaseuli, X. campestris pv. phaseoli var. fuscans, X. campestris pv. cajani, X. campestris pv. vignicola, X. campestris pv. dfdfae and X. campestris pv. glycines. At least six X. campestris pathovars were heterogeneous, displaying two or more protein electrophoretic types: X. campestris pv. dfaljhe, X. campestris pv. diefenbachiae, X. campestris pv. juglandis, X. campestris pv. poinsettiicola, X. campestris pv. vesicatoria and X. campestris pv. vignicola. A database of SDS-protein patterns provides a valuable tool for the identification of unknown xanthomonads.

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Section: Sds-page Of Whole-cell Proteinsmentioning

confidence: 94%

Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins

Vauterin

Swings

Kersters

1991

Journal of General Microbiology

100

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“…Later, more exact methods were applied, and numerical methods developed [4]. These involved calculation of similarities and clustering, applying the Pearson product correlation coefficient, coupled to the unweighted pairgroup with the arithmetic averages (UPGMA) method for clustering [5-81. Recently, a coefficient was constructed in which peak position and intensity are taken into account [9]. In AMBIS (automated microbiology identification system) bacterial strains are correlated by numerical analysis of the SDS-PAGE patterns of radiolabeled intracellular proteins [lo].…”

Section: Introductionmentioning

confidence: 99%

Protein profile characterization of bacterial lysates by capillary electrophoresis

et al. 1998

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A fast and reproducible method was developed to characterize cell lysates by their electrophoretic profiles using capillary electrophoresis (CE). Characteristic and reproducible patterns were recorded for each bacterial strains when "dynamic sieving" CE, using a polymer solution in the capillary, was applied to distinguish four strains of the Enterobacteriaceae family. The electropherograms showed distinct differences when comparing them to the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles. This is certainly a result of the differences in the separation principles and in the detection methods of the two techniques.

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“…This was in contrast to results obtained with 1-dimensional protein electrophoresis (27). Protein profiles have also proven useful for differentiation of other members of Pasteurcttaccae at both species and subspecies levels (1,66).…”

Section: Cell Protein Analysesmentioning

confidence: 99%

Recent approaches to the chemotaxonomy of the Actinobacillus‐Haemophilus–Pasteurella group(family Paseurellaceae)

Olsen¹

1993

Oral Microbiology and Immunology

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Many members of the Actinobacillus-Haemophilus-Pasteurella group (family Pasteurellaceae) have been misclassified. This article reviews the chemotaxonomic characters that recently have been provided to improve the taxonomy of Pasteurellaceae. These include fatty acids of whole cells, of lipopolysaccharides and of single colonies, together with sugar contents of whole cells, of whole defatted cells, of lipopolysaccharides and of single colonies. This article also reviews taxonomy aided by distribution of proteins in whole cells and outer membranes, distribution of enzymes in outer membrane vesicles and in whole cells, bacteriolysis induced by ethylenediaminetetraacetic acid and hen eggwhite lysozyme and the distribution of respiratory quinones. Furthermore, an overview of characters obtained through studies on genetic transformation, restriction enzyme analysis, restriction fragment length polymorphism, DNA-DNA hybridization, DNA-rRNA hybridization, and 16S rRNA sequencing is given.

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Comparison of whole-cell protein electrophoretic profiles of Haemophilus influenzae: implementation of a microcomputer mainframe linked system and description of a new similarity coefficient

Cited by 9 publications

References 10 publications

Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins

Grouping of Xanthomonas campestris pathovars by SDS-PAGE of proteins

Protein profile characterization of bacterial lysates by capillary electrophoresis

Recent approaches to the chemotaxonomy of the Actinobacillus‐Haemophilus–Pasteurella group(family Paseurellaceae)

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