Comparison of X‐ray vs. gamma irradiation of CPDA‐1 red cells

Janatpour, Kim; Denning, L.M; Nelson, Karen; Betlach, B.; MacKenzie, Malcolm R.; Holland, Paul V.

doi:10.1111/j.1423-0410.2005.00699.x

Cited by 63 publications

(76 citation statements)

References 7 publications

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“…Thus, X-ray irradiators are an alternative for the gamma-ray irradiator and are being increasingly used due to their low cost and absence of a radioactive source (6,7). Other factors such as no facility-licensing requirements, and less rigorous and easier maintenance also add to the advantages of an X-ray unit (2,8).…”

Section: Introductionmentioning

confidence: 99%

Biological X-ray irradiator characterization for use with small animals and cells

Bruno

Mazaro

Amaral

et al. 2017

Braz J Med Biol Res

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This study presents the characterization of an X-ray irradiator through dosimetric tests, which confirms the actual dose rate that small animals and cells will be exposed to during radiobiological experiments. We evaluated the linearity, consistency, repeatability, and dose distribution in the positions in which the animals or cells are placed during irradiation. In addition, we evaluated the performance of the X-ray tube (voltage and tube operating current), the radiometric survey (leakage radiation) and safety devices. The irradiator default setting was established as 160 kV and 25 mA. Tests showed that the dose rate was linear overtime (R2=1) and remained stable for long (constant) and short (repeatability) intervals between readings. The mean dose rate inside the animal cages was 1.27±0.06 Gy/min with a uniform beam of 95.40% (above the minimum threshold guaranteed by the manufacturer). The mean dose rate inside the cell plates was 0.92±0.19 Gy/min. The dose rate dependence with tube voltage and current presented a quadratic and linear relationship, respectively. There was no observed mechanical failure during evaluation of the irradiator safety devices and the radiometric survey obtained a maximum ambient equivalent dose rate of 0.26 mSv/h, which exempts it from the radiological protection requirements of the International Atomic Energy Agency. The irradiator characterization enables us to perform radiobiological experiments, and assists or even replaces traditional therapy equipment (e.g., linear accelerators) for cells and small animal irradiation, especially in early research stages.

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Section: Introductionmentioning

confidence: 99%

Biological X-ray irradiator characterization for use with small animals and cells

Bruno

Mazaro

Amaral

et al. 2017

Braz J Med Biol Res

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“…A previous report indicates that storage of irradiated RBCs increases the RBC hemolysis and the levels of free Hb [23], and alters the homeostasis in the sodiumpotassium balance [50]. These effects suggest an injury in the biophysical properties of the cell membrane and the subsequent alterations in the rheological parameters of RBCs.…”

Section: Rbcs Morphologic Analysismentioning

confidence: 97%

Carboxylated nanodiamond and re‐oxygenation process of gamma irradiated red blood cells

Acosta-Elías

Sarabia-Sainz

Pedroso‐Santana

et al. 2015

Physica Status Solidi (a)

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, Phone: þ52 662 259 2156, Fax: þ52 662 212 6649 Nanodiamonds (NDs) possess exceptional physical, chemical, and biological properties, which make them suitable for potential biomedical applications. They are biocompatible and their usefulness as effective Raman/fluorescence probes for labeling as well as for drug delivery has been demonstrated. Related to their biocompatibility, the interaction between NDs and red blood cells (RBCs) is of great interest. In this work, the influence of carboxylated NDs (cNDs) in the re-oxygenation capability of both g-irradiated and stored RBCs was studied. The standard 25 Gy g dose recommended to prevent transfusion associated graft-versus-host disease was used. A 5-day maximum storage time was used to evaluate the "storage lesion". The hemoglobin (Hb) oxygenation state was assessed by Raman microspectroscopy and the morphologic changes on cells were tracked by optical imaging. Our results show that irradiated RBCs have a better re-oxygenation capability and morphological recovery when they are in presence of cNDs.

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“…[66][67][68][69][70] This reduced viability has raised questions concerning the maximum storage time for RBCs after irradiation. The viability in vivo of irradiated RBCs, evaluated as the 24-hour recovery, is reduced during storage compared with that of nonirradiated RBCs.…”

Section: Red Blood Cellsmentioning

confidence: 99%

Irradiated Products

Luban¹,

Wong²

2007

Blood Banking and Transfusion Medicine

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Comparison of X‐ray vs. gamma irradiation of CPDA‐1 red cells

Abstract: Small differences in RBC membrane permeability are found between gamma-irradiated and X-ray-irradiated units. However, these differences are not likely to be clinically important.

Cited by 63 publications

References 7 publications

Biological X-ray irradiator characterization for use with small animals and cells

Biological X-ray irradiator characterization for use with small animals and cells

Carboxylated nanodiamond and re‐oxygenation process of gamma irradiated red blood cells

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