Comparison of Xpert MTB/RIF Assay and the Conventional Sputum Microscopy in Detecting<i>Mycobacterium tuberculosis</i>in Northern Thailand

Pinyopornpanish, Kanokwan; Chaiwarith, Romanee; Pantip, Chansom; Keawvichit, Rassamee; Wongworapat, Kanlaya; Khamnoi, Phadungkiat; Supparatpinyo, Khuanchai; Sirisanthana, Thira

doi:10.1155/2015/571782

Cited by 37 publications

(51 citation statements)

References 17 publications

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“…Our results are compatible with those of these previous studies; the sensitivity of Xpert assay to detect M. tuberculosis was 96.4% in acid-fast positive sputum specimens. Results similar to those in our study were obtained in Saudi Arabia, Pakistan, Thailand, and Turkey [16][17][18][19]. Our study and other studies all confirm that positive acid-fast smear correlated well with Xpert assay and TB culture.…”

Section: Discussionsupporting

confidence: 91%

Comparison of GeneXpert MTB/RIF and conventional methods for the diagnosis of tuberculosis in Kosovo

Bajrami

Mulliqi

Kurti

et al. 2016

J Infect Dev Ctries

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Introduction: Tuberculosis (TB) is a major public health problem worldwide, with the highest mortality occurring in developing countries. The burden of TB in Kosovo is among the highest in Europe. The aim of this study was to compare Cepheid GeneXpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis complex (MTBC) and rifampin (RIF) resistance with conventional methods. Methodology: A cross-sectional design to evaluate diagnostic tests was carried out at the Department of Microbiology, National Institute of Public Health of Kosovo and Lung Clinic, from January to June 2014. The detection of MTBC and RIF resistance using the Xpert MTB/RIF assay was assessed in 116 specimens received from 110 patients suspected of having TB and compared with conventional smear microscopy and culture methods. Results: Fifty-eight patients (52.7%) were male, and the mean age was 48.6 ± 18.1 years. Twenty-nine patients (26.4%) had underlying lung diseases. Of the 116 specimens investigated, 28 (24.1%) were MTBC-positive by culture, while 34 (29.3%) were positive by Xpert assay. Two samples showed false-negative Xpert results. Compared with culture, the Xpert assay achieved 82.3% (95% CI: 65.5%-93.2%) sensitivity, and 97.6% (95% CI: 91.5%-99.7%) specificity. GeneXpert could detect 11.7% and 50% additional positive cases as compared to LowensteinJensen culture and smear microscopy, respectively. Three cases with resistance to rifampin were detected from clinical isolates. Conclusions: The GeneXpert MTB/RIF assay is a helpful tool for rapid diagnosis and prompt treatment of TB.

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Section: Discussionsupporting

confidence: 91%

Comparison of GeneXpert MTB/RIF and conventional methods for the diagnosis of tuberculosis in Kosovo

Bajrami

Mulliqi

Kurti

et al. 2016

J Infect Dev Ctries

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“…In study of Pakistan, a total 403 samples were analyzed of which some are extra pulmonary specimens. The specificity of the current study was higher compared to specificity 86.4% of study conducted in Thailand [18]. The difference might be due to small sample size (109) and the gold standard used.…”

Section: Discussioncontrasting

confidence: 66%

Comparison of GeneXpert against Light-Emitting Diode Fluorescent Microscopy for the Diagnosis of Pulmonary Tuberculosis in Addis Ababa, Ethiopia

Taddese¹,

Desalegn²,

Misganaw³

et al. 2018

J Microb Biochem Technol

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“…For a sample to be positive, a bacterial load of 10 4 organisms is required while as identification of TB bacilli by GeneXpert require 131CFU/ml of sample. 13,14 Studies have shown that GeneXpert increased TB detection rate by 23 to 60% among culture confirmed cases while as in our study it was 47%. Culture methods like LJ medium and liquid culture are the gold standard tests for the detection of Mycobacterium tuberculosis.…”

supporting

confidence: 47%

Evaluation of smear microscopy and geneXpert for the rapid diagnosis of pulmonary and extrapulmonary tuberculosis in a tertiary care hospital in North India: a descriptive prospective study

et al. 2018

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Background: Tuberculosis is a global health problem associated with high morbidity and mortality. Rapid diagnosis of tuberculosis is essential for early disease management. Conventional methods like microscopy and culture are associated with low sensitivity and longer time to positivity respectively. The GeneXpert is an integrated device for the rapid detection of Mycobacterium tuberculosis and its sensitivity to rifampicin. We evaluated the performance of gene expert MTB/ RIF assay for the diagnosis of pulmonary and extrapulmonary tuberculosis.Methods: A prospective cross sectional study was carried out in the Department of Microbiology. Samples were subjected to smear microscopy by ZN staining, culture on solid (LJ) and liquid media (BacT Alert) and GeneXpert assay.Results: 122 pulmonary samples and 153 extrapulmonary samples collected from 275 patients were included in the study. Out of these, 48 samples were positive by both culture and Xpert assay and 2 samples were culture positive only. Out of 225 culture negative samples, 3 were positive by GeneXpert. The sensitivity for GeneXpert was much higher compared to smear micrscopy (96 Vs 46% respectively). The Xpert assay also detected 3 rifampcin resistant cases.Conclusions: The test appeared to be as sensitive as culture for the detection of tuberculosis in smear positive, smear negative and extrapulmonary tuberculosis. We recommend the use of GeneXpert assay for the early detection of tuberculosis. We conclude that the test is simple and routine staff can perform the test with minimal training.

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Comparison of Xpert MTB/RIF Assay and the Conventional Sputum Microscopy in DetectingMycobacterium tuberculosisin Northern Thailand

Cited by 37 publications

References 17 publications

Comparison of GeneXpert MTB/RIF and conventional methods for the diagnosis of tuberculosis in Kosovo

Comparison of GeneXpert MTB/RIF and conventional methods for the diagnosis of tuberculosis in Kosovo

Comparison of GeneXpert against Light-Emitting Diode Fluorescent Microscopy for the Diagnosis of Pulmonary Tuberculosis in Addis Ababa, Ethiopia

Evaluation of smear microscopy and geneXpert for the rapid diagnosis of pulmonary and extrapulmonary tuberculosis in a tertiary care hospital in North India: a descriptive prospective study

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