“…D-Glucose was used as the standard in calibrating the preceding tests. Protein content was determined by the dye-binding technique of Bradford (1976) using 0.5 mL of sample and 4.5 mL of Coomassie Blue G-250 reagent (Stanlake, 1986), with bovine serum albumin (Dade Diagnostic, Inc., Aquado, Puerto Rico) serving as the standard.…”