Comparison ofin Vitroandin VivoInfectivity of Different Clade B HIV-1 Envelope Chimeric Simian/Human Immunodeficiency Viruses inMacaca mulatta

Bogers, Willy; Dubbes, Rob; Haaft, Peter ten; Niphuis, Henk; Cheng‐Mayer, Cecilia; Stahl–Hennig∥, Christiane; Hunsmann, Gerhard; Kuwata, Takeo; Hayami, Masanori; Jones, Sue; Almond, Neil; Stott, Jim; Rosenwirth, Brigitte; Heeney, Jonathan L.

doi:10.1006/viro.1997.8744

Cited by 27 publications

(23 citation statements)

References 26 publications

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“…Despite a tenfold lower HIV-1 Han# inoculum used for ch-On as compared to ch-Su.A, the RNA peak levels in plasma were 7n5 times higher. This result indicates that a higher virus inoculum does not necessarily result in higher viraemia, confirming our earlier observations in the SIV\SHIV rhesus monkey model (Bogers et al, 1997 a). The numbers of proviral DNA-containing cells in both PBMC and LNMC from ch-Su.A and ch-On were similar despite a tenfold difference in virus inoculum dose and this level remained relatively persistent.…”

Section: Discussionsupporting

confidence: 89%

“…Furthermore, in addition to the antibody titres observed, HIV-1 Han# -specific neutralizing antibodies were detected 6 months post-infection (unpublished). Another possible reason for the absence of plasma antigen might be the presence of suppressor factors able to suppress HIV-1 replication in provirus-containing cells (Bogers et al, 1997 b ;Heeney et al, 1996).…”

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confidence: 99%

“…It is possible to achieve high virus loads in vivo with virus inocula of low TCID &! \ml values (Bogers et al, 1997 a).…”

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confidence: 99%

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Characteristics of primary infection of a European human immunodeficiency virus type 1 clade B isolate in chimpanzees.

Bogers

Koornstra

Dubbes

et al. 1998

Journal of General Virology

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The aim of the study was to select, from a panel of candidate European human immunodeficiency virus type 1 (HIV-1) clade B primary virus isolates, one isolate based on replication properties in chimpanzee peripheral blood mononuclear cells (PBMC). Secondly, to evaluate the in vivo kinetics of primary infection of the selected isolate at two different doses in two mature, outbred chimpanzees (Pan troglodytes). Four different low passage, human PBMC-cultured ' primary ' HIV-1 isolates with European clade B consensus sequence were compared for their ability to replicate in vitro in chimpanzee versus human PBMC. The isolate which yielded the highest titre and most vigorous cytopathic effect in chimpanzee PBMC was evaluated for coreceptor usage and chosen for evaluation in vivo. Only the

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Section: Discussionsupporting

confidence: 89%

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confidence: 99%

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Characteristics of primary infection of a European human immunodeficiency virus type 1 clade B isolate in chimpanzees.

Bogers

Koornstra

Dubbes

et al. 1998

Journal of General Virology

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“…We postulate that systemic infection is achieved once the virus inoculum exceeds a predetermined threshold, which differs for the various routes of inoculation and is age dependent, according to our new data. However, once this threshold has been surpassed and systemic infection is established, there is no association between the inoculum size and peak viral RNA; this lack of a dose response has been observed previously (6) and is not surprising for an outbred population of primates.…”

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confidence: 50%

Older Rhesus Macaque Infants Are More Susceptible to Oral Infection with Simian-Human Immunodeficiency Virus 89.6P than Neonates

Chenine

Ferrantelli

Hofmann‐Lehmann

et al. 2005

J Virol

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. Using a stock of pathogenic simianhuman immunodeficiency virus, SHIV89.6P, we compared the 50% animal infectious dose needed to achieve systemic infection after oral challenge in newborn and older infant or juvenile rhesus macaques. Unexpectedly, the older monkeys required a 150-fold-lower virus challenge dose than the neonates (P ‫؍‬ 3.3 ؋ 10 ؊5 ). In addition, at least 60,000 times more virus was needed to achieve systemic infection in neonates by the oral route than by the intravenous route (P < 1 ؋ 10 ؊5 ). Thus, route of inoculation and age are important determinants of SHIV89.6P infectivity in rhesus macaques.

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“…Simian-human immunodeficiency virus (SHIV) strains are chimeric viruses constructed from the pathogenic simian immunodeficiency virus (SIV) clone SIVmac239, in which the env, tat, and rev genes had been replaced by the corresponding regions of human immunodeficiency virus type 1 (HIV-1); most SHIVs also contain vpu (7,21,22,25,27,31,35,47). SHIV infection of rhesus macaques has been an invaluable in vivo model to study the role of HIV-1 envelopes in transmission and pathogenesis as well as to evaluate the efficacy of candidate vaccines based on HIV-1 envelope glycoproteins, which specify cell tropism and coreceptor usage and are also primary targets of the immune response.…”

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confidence: 99%

Molecularly Cloned SHIV-1157ipd3N4: a Highly Replication- Competent, Mucosally Transmissible R5 Simian-Human Immunodeficiency Virus Encoding HIV Clade Cenv

Song

Chenine

Rasmussen

et al. 2006

J Virol

150

221

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Human immunodeficiency virus type 1 (HIV-1) clade C causes >50% of all HIV infections worldwide, and an estimated 90% of all transmissions occur mucosally with R5 strains. A pathogenic R5 simian-human immunodeficiency virus (SHIV) encoding HIV clade C env is highly desirable to evaluate candidate AIDS vaccines in nonhuman primates. To this end, we generated SHIV-1157i, a molecular clone from a Zambian infant isolate that carries HIV clade C env. SHIV-1157i was adapted by serial passage in five monkeys, three of which developed peripheral CD4 ؉ T-cell depletion. After the first inoculated monkey developed AIDS at week 137 postinoculation, transfer of its infected blood to a naïve animal induced memory T-cell depletion and thrombocytopenia within 3 months in the recipient. In parallel, genomic DNA from the blood donor was amplified to generate the late proviral clone SHIV-1157ipd3. To increase the replicative capacity of SHIV1157ipd3, an extra NF-B binding site was engineered into its 3 long terminal repeat, giving rise to SHIV1157ipd3N4. This virus was exclusively R5 tropic and replicated more potently in rhesus peripheral blood mononuclear cells than SHIV-1157ipd3 in the presence of tumor necrosis factor alpha. Rhesus macaques of Indian and Chinese origin were next inoculated intrarectally with SHIV-1157ipd3N4; this virus replicated vigorously in both sets of monkeys. We conclude that SHIV-1157ipd3N4 is a highly replication-competent, mucosally transmissible R5 SHIV that represents a valuable tool to test candidate AIDS vaccines targeting HIV-1 clade C Env.

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Comparison ofin Vitroandin VivoInfectivity of Different Clade B HIV-1 Envelope Chimeric Simian/Human Immunodeficiency Viruses inMacaca mulatta

Cited by 27 publications

References 26 publications

Characteristics of primary infection of a European human immunodeficiency virus type 1 clade B isolate in chimpanzees.

Characteristics of primary infection of a European human immunodeficiency virus type 1 clade B isolate in chimpanzees.

Older Rhesus Macaque Infants Are More Susceptible to Oral Infection with Simian-Human Immunodeficiency Virus 89.6P than Neonates

Molecularly Cloned SHIV-1157ipd3N4: a Highly Replication- Competent, Mucosally Transmissible R5 Simian-Human Immunodeficiency Virus Encoding HIV Clade Cenv

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