This paper presents measures of target strength (TS; dB re 1 m 2 ) and models of TS vs. fork length (L; cm), i.e. TS ¼ 20log L ð Þ þ b 20 , for skipjack tuna associated with fish aggregating devices (FADs) in the Central Pacific Ocean. Measurements were made using 38-, 120-, and 200-kHz split-beam echosounders on a purse-seine workboat during fishing operations. To mitigate potential bias due to unresolved targets, TS measurements were rejected if they were not simultaneously detected with multiple echosounder frequencies in approximately the same location. The filtered TS and concomitantly sampled L data were used to estimate b 20 ¼ À76, À71, and À70.5 dB for 38, 120, and 200 kHz, respectively, using the method of least squares. For comparison, quasi-independent estimates of TS and b 20 were calculated from acoustic echointegration and catch data representing entire aggregations around the FADs. The results differed by 1 dB for all three frequencies. The sensitivities of these results to variations in fish morphology and behaviour were explored using a simulation of TS for fish without swimbladders. The utility of the results on acoustic properties of skipjack tuna and next research steps to achieve selective fishing at FADs are discussed.