Comparisons between nulliparous heifers and cows as oocyte donors for embryo production in vitro

Rizos, Dimitrios; Burke, Lisa A.; Duffy, Patrick E.; Wade, M.; Mee, John F.; O’Farrell, K.J.; MacSiúrtáin, Máirtı́n; Boland, M.P.; Lonergan, Patrick

doi:10.1016/j.theriogenology.2004.05.008

Cited by 47 publications

(41 citation statements)

References 30 publications

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“…In bovine, IVEP is especially interesting to mitigate the decrease in fertility suffered by dairy cattle during environmental heat stress conditions (Ambrose et al, 1999; -E-mail: miguel.silvestre@uv.es 455 Al-Katanani et al, 2002) or to take advantage of the ovum pick-up technique (OPU). In the latter case, a small number of oocytes per female are usually recovered after OPU (approximately three to six oocytes recovered per nonstimulated cow and session; Rizos et al, 2005;Chaubal et al, 2006). IVEP efficiency has increased in recent years, although the quality of these embryos remains lower than in embryos produced in vivo (reviewed by Rizos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Section: Introductionmentioning

confidence: 99%

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…Many factors have been shown to affect the oocyte's developmental potential, including follicle size (Lonergan et al 1994), health of the follicle (Blondin & Sirard 1995, Vassena et al 2003, phase of follicular wave (Hagemann 1999, Machatková et al 2004, hormonal stimulation (Blondin et al 2002; for review Sirard et al 2006), maturation environment (Warzych et al 2007; for review Sutton et al 2003), season (Al-Katanani et al 2002, Sartori et al 2002, nutrition (Fouladi-Nashta et al 2007) and age (Rizos et al 2005). Although previous studies support the notion that oocyte competence depends on multiple factors, it remains difficult to draw clear and reliable criteria for oocyte selection.…”

Section: Introductionmentioning

confidence: 99%

Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity

Torner¹,

Ghanem²,

Ambros³

et al. 2008

Reproduction

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Oocyte selection based on glucose-6-phosphate dehydrogenase (G6PDH) activity has been successfully used to differentiate between competent and incompetent bovine oocytes. However, the intrinsic molecular and subcellular characteristics of these oocytes have not yet been investigated. Here, we aim to identify molecular and functional markers associated with oocyte developmental potential when selected based on G6PDH activity. Immature compact cumulus-oocyte complexes were stained with brilliant cresyl blue (BCB) for 90 min. Based on their colouration, oocytes were divided into BCB K (colourless cytoplasm, high G6PDH activity) and BCB C (coloured cytoplasm, low G6PDH activity). The chromatin configuration of the nucleus and the mitochondrial activity of oocytes were determined by fluorescence labelling and photometric measurement. The abundance and phosphorylation pattern of protein kinases Akt and MAP were estimated by Western blot analysis. A bovine cDNA microarray was used to analyse the gene expression profiles of BCB C and BCB K oocytes. Consequently, marked differences were found in blastocyst rate at day 8 between BCB C (33.1G3.1%) and BCB K (12.1G1.5%) oocytes. Moreover, BCB C oocytes were found to show higher phosphorylation levels of Akt and MAP kinases and are enriched with genes regulating transcription (SMARCA5), cell cycle (nuclear autoantigenic sperm protein, NASP ) and protein biosynthesis (RPS274A and mRNA for elongation factor 1a, EF1A). BCB K oocytes, which revealed higher mitochondrial activity and still nucleoli in their germinal vesicles, were enriched with genes involved in ATP synthesis (ATP5A1), mitochondrial electron transport (FL405), calcium ion binding (S100A10) and growth factor activity (bone morphogenetic protein 15, BMP15). This study has evidenced molecular and subcellular organisational differences of oocytes with different G6PDH activity.

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“…The number of oocytes recovered and classified as category III was the largest (2.3 ± 0.32), followed by category II (1.3 ± 0.19), category I (1.3 ± 0.20), category IV (1.2 ± 0.25), category VI (0.5 ± 0.15) and category V (0.2 ± 0.07). The number of oocytes recovered per OPU session in our experiment was similar to the values reported by Bungartz et al (1995) and higher than those reported by other authors previously (Hasler et al, 1995;Goodhand et al, 1999;Rizos et al, 2005;Chaubal et al, 2006;Lopes et al, 2006). Regarding the oocyte morphology classification, the absence of standardized classification make difficult to compare results between different scientific publications.…”

Section: Opu-derived Oocytesupporting

confidence: 87%

“…These in vitro systems usually culture approximately 40-50 oocytes/embryos in 400-500 µL of medium, obtaining blastocyst rates around 30-40% Gordon, 2003). However, for commercial purposes such as use in OPU, where it is usually necessary just to keep the immature oocytes from one donor together, a small number of oocytes/embryos can be cultured (approximately three to six oocytes recovered per non-stimulated cow; Rizos et al, 2005;Chaubal et al, 2006). It has been widely reported that in vitro embryo development in bovine and other mammalian species tends to be suppressed in cultures with a single or low number of embryos (in mouse: Paria and Dey, 1990;Canseco et al, 1992;Lane and Gardner, 1992;Kato and Tsunoda, 1994;in bovine: Palma et al, 1992;Ferry et al, 1994;Keefer et al, 1994;Blondin and Sirard, 1995;Carolan et al, 1996;Donnay et al, 1997;O`Doherty et al, 1997;Ward et al, 2000;Oyamada and Fukui, 2004;Pereira et al, 2005;Fujita et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem

Serrano¹

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Esta tesis ha sido propuesta para Mención Internacional en el título de Doctor en virtud de la siguiente estancia de investigación en el extranjero:• Institute of farm animal genetic, Friedrich-Loeffler Institute, Mariensee, Alemania.Supervisor: Dr. Detlef Rath.Periodo de duración: del 4 de mayo al 15 de octubre de 2009. AGRADECIMIENTOSLas tesis doctorales tienen una cosa en común que las hace épicas; buena parte del camino debe recorrerse en solitario. Sin embargo, sin la ayuda y la hospitalidad de las personas que vamos encontrando por el largo y sinuoso viaje iniciático que supone una tesis doctoral, nos hariamos viejos sin llegar lejos. Sirvan estas líneas para mostrar mi agradecimiento a todos los compañeros y amigos que me han ayudado y han hecho que esta tesis fuera posible.En primer lugar, a mis directores de tesis Miguel Ángel Silvestre y Ignacio Salvador, por darme la posibilidad de trabajar y aprender a su lado y de progresar en el mundo de la investigación.A mis compañeros de laboratorio, Elena Megual, Empar Raga, Empar García-Rosselló, Richard Lopera, Javier Ahumada, Manuel Francisco Simao y Ángel Picó, por su inestimable contribución durante la realización de los experimentos.Al Profesor Detlef Rath, por haberme acogido desinteresadamente en su grupo de investigación y haber puesto a mi disposición los medios necesarios para aprender nuevas técnicas y realizar experimentos, y al Profesor Salvador Ruiz y a los compañeros del Institut für Nutztiergenetik que me ayudaron durante mi estancia en Mariensee, especialmente a Klaus-GerdHadeler, Javier Zaraza y Miguel Velázquez.Al Profesor Jesus Yáñiz y al Profesor Fernando López-Gatius, por su valiosa asistencia técnica en los primeros pasos de esta tesis.A la Asociación Frisona Valenciana y a la Granja S.A.T. San Ramón, por el apoyo prestado durante la gestación y el desarrollo de este proyecto.A los compañeros y amigos del CITA, por su contribución directa/o indirecta a este trabajo y por haber compartidos tantos buenos momentos.Por último, a mis amigos y a mi familia, en especial a mis padres, por su apoyo, ánimo y estima incondicional. Cuando emprendas el viaje hacia

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Comparisons between nulliparous heifers and cows as oocyte donors for embryo production in vitro

Cited by 47 publications

References 30 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity

Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem

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