Comparisons Between X-ray Film- and Phosphorescence Imaging-Based Autoradiography for the Visualization of Herbicide Translocation

Wehtje, Glenn; Miller, Michael E.; Grey, Timothy L.; Brawner, William R.

doi:10.1614/wt-06-152.1

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…Plants were removed and glued onto 8 by 10-cm cardstock mounts. Mounts were covered in plastic wrap then placed in exposure cassettes (Amersham Biosciences) and pressed against phosphor imaging plates for approximately 24 h, then scanned with the use of the image analyzer, in a procedure described in depth by Wehtje et al (2007).…”

Section: Methodsmentioning

confidence: 99%

Physiological Basis for Differential Selectivity of Four Grass Species to Aminocyclopyrachlor

et al. 2015

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Aminocyclopyrachlor (AMCP) is a synthetic auxin herbicide used for broadleaf weed control in pasture and rangeland. The tolerance and fate of AMCP within pertinent grass species is not well understood. Research was conducted to establish the tolerance of four grass species to AMCP application and observe their absorption, translocation, and metabolism. Results indicate that tall fescue is the most tolerant of AMCP at rates required for weed control. Bahiagrass and bermudagrass are marginally tolerant, and cogongrass is the most sensitive. Tall fescue and bahiagrass absorbed more AMCP than bermudagrass and cogongrass, but cogongrass absorption is the most rapid and complete within 2 days after treatment (DAT). Cogongrass and bermudagrass translocated the least amount out of the target area, whereas bahiagrass and tall fescue translocated the most. Radioisotope imaging revealed that tall fescue may sequester absorbed AMCP in leaf tips. This sequestering may be the basis of the greater tolerance to AMCP by tall fescue relative to the other species evaluated. No metabolism of AMCP was detected in any grass species out to 42 DAT.

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Section: Methodsmentioning

confidence: 99%

Physiological Basis for Differential Selectivity of Four Grass Species to Aminocyclopyrachlor

et al. 2015

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“…This process involves exposure of plant specimens to a film containing a specialized coating for a period of 24 h. A 1-d exposure time with phosphorimaging gave comparable or better results than a 3-wk exposure time with X-ray film (Wehtje et al 2007). The film is then scanned by a laser, resulting in the development of an image, which is oftentimes 103 more sensitive compared with a X-ray autoradiograph.…”

Section: Phosphorimagingmentioning

confidence: 99%

Herbicide Absorption and Translocation in Plants using Radioisotopes

Nandula

Vencill

2015

Weed sci.

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2,4-D, discovered independently in the United States and Europe in the mid-1940s, was one of the first synthetic herbicides to be used selectively for weed control (Cobb and Reade 2010). Since then, several herbicides belonging to different chemical classes and possessing diverse mechanisms of action have been synthesized and marketed globally. Herbicides have vastly contributed to increasing world food, fiber, fuel, and feed production in an efficient, economic, and environmentally sustainable manner. Before receiving regulatory approval, all herbicides (pesticides) undergo rigorous testing for their toxicological, residual, physicochemical, and biological properties. Additionally, herbicides are suitably formulated to reach their target site and maximize their efficacy on target weeds while being safe on crops.

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“…Despite more expensive, the technique is also quicker. A single day of exposition of a plant on a phosphorus blade resulted on images with superior quality than the exposition for 3 weeks with the X-ray film [24].…”

Section: Herbicide Absorption and Translocation In Resistant Weedsmentioning

confidence: 99%

Procedures for Detection of Resistant Weeds Using 14C- Herbicide Absorption, Translocation, and Metabolism

Mendes¹,

Silveira²,

Inoue³

et al. 2017

Herbicide Resistance in Weeds and Crops

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Herbicide resistance mechanisms involve altered absorption, translocation, and metabolism of herbicides (i.e., glyphosate), and this is an important component in the study of herbicide resistance mechanisms as well. 14 C-herbicides are used in resistant weeds studies, since they provide some advantages in comparison with chemical measures, including greater sensitivity, stepwise description of a particular element in a metabolic system, herbicide position, detection through X-ray films and/or radio image, and liquid scintillation. However, an up-to-date, organized description and standardization of research procedures and methodology on the use of radioisotopes for detection of resistant weeds, through different mechanisms of absorption, translocation, and metabolism in comparison with susceptible weeds are lacking in the literature. Techniques that use 14 C such as tracers are extremely useful to study the herbicides behavior in the resistant weed, since the radiometric techniques offer the possibility of accurately determining very small amounts in a relatively short time. However, mechanism of resistance to herbicides in this resistant weed population compared with the susceptible population cannot be due to differential absorption, translocation, or metabolism of herbicide in weed; so other studies are necessary to elucidate the mechanism of herbicide resistance on weed population.

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Comparisons Between X-ray Film- and Phosphorescence Imaging-Based Autoradiography for the Visualization of Herbicide Translocation

Cited by 10 publications

References 16 publications

Physiological Basis for Differential Selectivity of Four Grass Species to Aminocyclopyrachlor

Physiological Basis for Differential Selectivity of Four Grass Species to Aminocyclopyrachlor

Herbicide Absorption and Translocation in Plants using Radioisotopes

Procedures for Detection of Resistant Weeds Using 14C- Herbicide Absorption, Translocation, and Metabolism

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