Comparisons of longissimus muscle metabolic enzymes and muscle fiber types in Korean and western pig breeds

Kim, Nam-Kuk; Lim, Jong-Hyun; Song, Min-Jin; Kim, Oun-Hyun; Park, Beom-Young; Kim, Myung-Jick; Hwang, Inho; Lee, Chang‐Soo

doi:10.1016/j.meatsci.2007.07.014

Cited by 50 publications

(29 citation statements)

References 24 publications

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“…Many previous reports have shown a relationship between muscle fiber type and meat quality (Kim et al, 2008;Ryu et al, 2008;Choi and Kim, 2009). Muscle fibers are the basic muscle units, which directly affect meat quality, especially tenderness, flavor, juiciness, water-holding capacity, etc.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with lean pigs, the LM of Chinese local pigs exhibited higher contents of oxidative muscle fibers, which may be associated with their excellent meat and slow growth. Many studies have shown that oxidative muscle fibers are positively related with IMF and tenderness (Kim et al, 2008;Choi and Kim, 2009), whereas glycolytic muscle fibers are related with pale, soft, and exudative meat (Franck et al, 2007). Although breed is one of the most important factors in determining meat quality, the ultimate reason for the different meat qualities is differences in muscle fiber type.…”

Section: Discussionmentioning

confidence: 99%

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Comparisons of different myosin heavy chain types, AMPK, and PGC-1α gene expression in the longissimus dorsi muscles in Bama Xiang and Landrace pigs

Huang

Jiang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Bama Xiang and Landrace pigs are the local fatty and lean breeds, respectively, in China. We compared differences in carcass traits, meat quality traits, and myosin heavy chain (MyHC) types in the longissimus dorsi muscles between Bama Xiang and Landrace pigs. This was done in pigs of the same age, using real-time PCR, to investigate the relationship between MyHC fiber types and carcass characteristics, meat quality traits, and the key factors regulating muscle fiber type. Bama Xiang pigs exhibited smaller size and slower growth than Landrace pigs (P < 0.01). We found that the superior meat quality, especially the high intramuscular fat (IMF) content in Bama Xiang pig, was related to elevated type I oxidative muscle fiber content (P < 0.01). In contrast, Landrace pig muscle had a higher glycolytic type IIb muscle fiber content (P < 0.01). MyHC I gene expression was significantly positively correlated with backfat thickness and IMF content (P < 0.01). MyHC IIb was significantly negatively correlated with IMF content (P < 0.05), and positively correlated with carcass yield (P < 0.05). AMP-activated protein kinase and peroxisome proliferatoractivated receptor-g coactivator-1a are suggested to be the two key factors regulating muscle fiber type in pigs. Our results indicate that muscle fiber composition is one of the key differences leading to the differences of meat quality between Bama Xiang and Landrace pigs. These results may provide a theoretical basis for further studies of the molecular mechanism underlying the excellent meat quality of the Bama Xiang pig.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Comparisons of different myosin heavy chain types, AMPK, and PGC-1α gene expression in the longissimus dorsi muscles in Bama Xiang and Landrace pigs

Huang

Jiang

et al. 2016

Genet. Mol. Res.

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“…This indicates that the Piau breed possesses a higher oxidative capacity than the Large White and Duroc breeds. Kim et al (2008) reported elevated expression of the oxidation-related metabolism gene NADH dehydrogenase in Korean naturalized pig muscle associated to meat quality indicated by a higher content of oxidative fibers and muscle fat, as well as redder meat color. Similar results were found by Hocquette et al (1998) for skeletal muscles with a higher fat content that was also more oxidative.…”

Section: Discussionmentioning

confidence: 99%

“…Breed probably accounts for most of the genetic factors affecting the muscle fiber composition. Significant differences in meat quality and metabolic profiles between commercial and naturalized pigs have been reported (Lin and Hsu, 2005;Wimmers et al, 2008;Kim et al, 2008). Therefore, the comparison of commercial and naturalized breeds might provide important information about the genetic factors that are responsible for meat quality.…”

Section: Introductionmentioning

confidence: 99%

Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds

Nascimento

Peixoto²,

Verardo³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breedrelated gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the Swiss-Prot protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds.

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“…Primer sequences were given in Table 1. The primers of four MyHC isoforms were designed according to Kim et al (2008). The reactions contained 1 3 SYBR Green real-time PCR Master Mix, 1 ml prediluted cDNA template and each primer at 200 nM in a 25 ml reaction volume.…”

Section: Methodsmentioning

confidence: 99%

Temporal expression of TnI fast and slow isoforms in biceps femoris and masseter muscle during pig growth

Yang

et al. 2010

Animal

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Biceps femoris (BF) and masseter muscle (MM) are the mixture of slow oxidative and fast-twitch fibres. Compared with MM, BF had the significantly higher expression of myosin heavy chain (MyHC) fast IIx and IIb isoforms (MyHCIIx and MyHCIIb), but lower expression of MyHC slow isoform (MyHCI) and fast IIa isoform (MyHCIIa). The objective of this study was to investigate the expression pattern of troponin I (TnI) slow-twitch isoform (TNNI1) and fast-twitch isoform (TNNI2) in BF and MM of Yorkshire and Meishan pigs which differed significantly in the growth rate. The expression of the TNNI1 and TNNI2 peaked at the postnatal 35 days in Yorkshire pigs and postnatal 60 days in Meishan pigs. The expression of TNNI1 and TNNI2 in Meishan pigs was significantly higher than that in Yorkshire pigs at the foetal 60 days, while the opposite occurred at postnatal 35 days. The expression ratio of TNNI1 relative to TNNI2 favoured TNNI2 expression in BF and MM regardless of Yorkshire and Meishan pigs. TNNI1 expression in MM was significantly higher than that in BF at 60, 120 and 180 days in Meishan pigs and at 120 and 180 days in Yorkshire pigs. On the contrary, no significant difference of TNNI2 expression in BF and MM was found except for Yorkshire pigs of 180 days. This study provided the foundation for future research on TnI isoforms as the model gene to study mechanisms of muscle fibre-specific gene regulation in pigs.

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Comparisons of longissimus muscle metabolic enzymes and muscle fiber types in Korean and western pig breeds

Cited by 50 publications

References 24 publications

Comparisons of different myosin heavy chain types, AMPK, and PGC-1α gene expression in the longissimus dorsi muscles in Bama Xiang and Landrace pigs

Comparisons of different myosin heavy chain types, AMPK, and PGC-1α gene expression in the longissimus dorsi muscles in Bama Xiang and Landrace pigs

Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds

Temporal expression of TnI fast and slow isoforms in biceps femoris and masseter muscle during pig growth

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