Duration discrimination in the range of milliseconds is essential for various aspects of behavior and individual differences. The present paper addresses important methodological issues, such as type of stimuli, type of task, method for threshold estimation, and temporal sensitivity of the psychophysical procedure, that should be borne in mind when developing a sensitive and reliable duration discrimination task. Furthermore, it introduces a psychophysical approach for the assessment of individual differences in duration discrimination of extremely brief intervals in the subsecond range. Monte Carlo simulations provide clear evidence that this task is sensitive enough to correctly detect a true difference between temporal stimuli as small as 2 ms with a high probability. Further, the distributional properties of individual performance scores obtained from 534 participants by means of the introduced duration discrimination task are presented.