Compartments with predominant ephrin‐B1 and EphB2/B4 expression are present alternately along the excurrent duct system in the adult mouse testis and epididymis

Gofur, M. R.; Ogawa, Kazushige

doi:10.1111/andr.12523

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…Activin signaling (INHBB-ACVR2A/1B/2B) can influence germ cell development through the phosphorylation of SMAD2/3(58). EphB/ephrin-B signaling (EFNB1/2-EPHB4/6) may modulate spermatogenesis and spermiation ( 59 ). Further analysis of the TFs downstream of these intercellular communications revealed that most of these TFs were related to spermatogenesis (see Figure 2E ).…”

Section: Resultsmentioning

confidence: 99%

“…A case study on human testicular cells indicated prominent signal crosstalk between ST cells and SSCs. Most of the inferred intercellular communications (41/47) from ST cells to SSCs have been implicated in spermatogenesis or SSC differentiation ( 53 , 54 , 56 , 58 , 59 ), and some of them were confirmed by immunofluorescence assays. Intercellular communication analysis also indicated that intercellular crosstalk among germ cells may play roles in spermatogenesis, and a communication axis from P to SSCs (GDF5-BMPR1B-SMAD1) was confirmed by immunostaining.…”

Section: Discussionmentioning

confidence: 95%

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CellCall: integrating paired ligand–receptor and transcription factor activities for cell–cell communication

Zhang

Liu

et al. 2021

Nucleic Acids Research

153

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With the dramatic development of single-cell RNA sequencing (scRNA-seq) technologies, the systematic decoding of cell-cell communication has received great research interest. To date, several in-silico methods have been developed, but most of them lack the ability to predict the communication pathways connecting the insides and outsides of cells. Here, we developed CellCall, a toolkit to infer inter- and intracellular communication pathways by integrating paired ligand-receptor and transcription factor (TF) activity. Moreover, CellCall uses an embedded pathway activity analysis method to identify the significantly activated pathways involved in intercellular crosstalk between certain cell types. Additionally, CellCall offers a rich suite of visualization options (Circos plot, Sankey plot, bubble plot, ridge plot, etc.) to present the analysis results. Case studies on scRNA-seq datasets of human testicular cells and the tumor immune microenvironment demonstrated the reliable and unique functionality of CellCall in intercellular communication analysis and internal TF activity exploration, which were further validated experimentally. Comparative analysis of CellCall and other tools indicated that CellCall was more accurate and offered more functions. In summary, CellCall provides a sophisticated and practical tool allowing researchers to decipher intercellular communication and related internal regulatory signals based on scRNA-seq data. CellCall is freely available at https://github.com/ShellyCoder/cellcall.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 95%

CellCall: integrating paired ligand–receptor and transcription factor activities for cell–cell communication

Zhang

Liu

et al. 2021

Nucleic Acids Research

153

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“…This segment pair revealed that the upregulated DEGs may play important roles in toxicant detection and sperm maturation; Taste 2 Receptor Member 42 (TASR42), Epididymal Protein 3A (EDDM3A), testisin (PRSS21) (Governini et al, 2020;Kirchhoff et al, 1998;Netzel-Arnett et al, 2009), transcription factor (Paired Box 2, PAX2) (Lowen et al, 2001), and restructuring of sperm head plasma membrane, which is a form of prominent maturational changes (Sulfhydryl oxidase 1, QSOX1) (Wang et al, 2018). EPHB6 acts as sensor for unidentified chemicals and also participates in cell adhesion as well as sperm transport (Gofur & Ogawa, 2019) F I G U R E 5 Gene Ontology (GO) categories of all genes and differentially expressed genes (DEGs) of cattleyak epididymis. The number and percentage of genes in biological process, cellular component, and molecular function are indicated for all genes and DEGs, respectively.…”

Section: Caput Versus Corpusmentioning

confidence: 99%

RNA‐Seq reveals the functional specificity of epididymal caput, corpus, and cauda genes of cattleyak

Xia

Adjei

Wang

et al. 2022

Animal Science Journal

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The first filial generation of the cattleyaks demonstrates hybrid vigor; however, the male cattleyaks are infertile and restrict productivity and breeding. The discovery of genes in a segment-specific approach offers valuable information and understanding concerning fertility status, yet the biology of cattleyak epididymis is still progressing.Comparative transcriptome analysis was performed on segment pairs of cattleyak epididymis. The caput versus corpus epididymis provided the highest (57.8%) differentially expressed genes (DEGs), corpus versus cauda (25.1%) followed, whereas caput versus cauda pair (17.1%) had the least DEGs. The expression levels of genes coding EPHB6, TLR1, MUC20, MT3, INHBB, TRPV5, EI24, PAOX, KIF12, DEPDC5, and KRT25, which might have the potentials to regulate the homeostasis, innate immunity, differentiation, motility, transport, and sperm maturation-related function in epididymal cells, were downregulated in the distal segment of epididymis. Top enriched KEGG pathways included mTOR, axon guidance, and taste transduction signaling pathways. EIF4B, EPHB6, and TAS2R42 were enriched in the pathways, respectively.Identifying key, new, and unexplored DEGs among the epididymal segments and further analyzing them could boost cattleyak fertility by maximizing sperm quality from genetically better sires and also facilitate better understanding of the epididymal biology.

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“…This review aims to illustrate the potential for developing targeted vehicles, targeting methods, and therapeutic cargo to modulate Eph/ephrin signaling (Figure 2). Pharmaceuticals 2020, 13, x FOR PEER REVIEW 3 of 18 disorders [4,5,9], musculoskeletal growth and metabolism diseases [42][43][44][45], ischemic tissue injury [46][47][48][49][50][51][52][53][54][55][56] and malignancies [7,57,58], reproductive/fertility illnesses [59][60][61][62] and organ fibrosis [10,63].…”

Section: Eph/ephrin Is a Ubiquitous Therapeutic Targetmentioning

confidence: 99%

Harnessing the Power of Eph/ephrin Biosemiotics for Theranostic Applications

Hughes

Virag

2020

Pharmaceuticals

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Comprehensive basic biological knowledge of the Eph/ephrin system in the physiologic setting is needed to facilitate an understanding of its role and the effects of pathological processes on its activity, thereby paving the way for development of prospective therapeutic targets. To this end, this review briefly addresses what is currently known and being investigated in order to highlight the gaps and possible avenues for further investigation to capitalize on their diverse potential.

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Compartments with predominant ephrin‐B1 and EphB2/B4 expression are present alternately along the excurrent duct system in the adult mouse testis and epididymis

Abstract: Overall, this study represents the first analysis of EphB receptor and ephrin-B ligand expression in the normal adult testis and epididymis.

Cited by 11 publications

References 40 publications

CellCall: integrating paired ligand–receptor and transcription factor activities for cell–cell communication

CellCall: integrating paired ligand–receptor and transcription factor activities for cell–cell communication

RNA‐Seq reveals the functional specificity of epididymal caput, corpus, and cauda genes of cattleyak

Harnessing the Power of Eph/ephrin Biosemiotics for Theranostic Applications

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