Compatibility of Mammalian Cells on Surfaces of Poly(dimethylsiloxane)

Lee, Jessamine Ng; Jiang, Xingyu; Ryan, Declan; Whitesides, George M.

doi:10.1021/la048562+

Cited by 349 publications

(313 citation statements)

References 62 publications

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“…However, these silica filler are dimethylvinylated and trimethylated silica [24], and different from the silica nanoparticles (A380 and MCM-41) used in this study. Moreover, during the curing process, the vinyl of the silica filler and the silicon hydride groups of the curing agent would undergo a hydrosilylation reaction to form a Si-C bond [24]. Therefore, it is believed that the silica nanoparticles used in this work would not interact with the silica filler, but with the whole three-dimensional PDMS network.…”

Section: Resultsmentioning

confidence: 99%

Effects of Fumed and Mesoporous Silica Nanoparticles on the Properties of Sylgard 184 Polydimethylsiloxane

Liu

Zong

et al. 2015

Micromachines

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Abstract:The effects of silica nanoparticles on the properties of a commonly used Sylgard 184 polydimethylsiloxane (PDMS) in microfluidics were systemically studied. Two kinds of silica nanoparticles, A380 fumed silica nanoparticles and MCM-41 mesoporous silica nanoparticles, were individually doped into PDMS, and the properties of PDMS with these two different silica nanoparticles were separately tested and compared. The thermal and mechanical stabilities of PDMS were significantly enhanced, and the swelling characteristics were also improved by doping these two kinds of nanoparticles. However, the transparency of PDMS was decreased due to the light scattering by nanoparticles. By contrast, PDMS/MCM-41 nanocomposites showed a lower coefficient of thermal expansion (CTE) owing to the mesoporous structure of MCM-41 nanoparticles, while PDMS/A380 nanocomposites showed a larger elastic modulus and better transparency due to the smaller size of A380 nanoparticles. In addition, A380 and MCM-41 nanoparticles had the similar effects on the swelling characteristics of PDMS. The swelling ratio of PDMS in toluene was decreased to 0.68 when the concentration of nanoparticles was 10 wt %.

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Section: Resultsmentioning

confidence: 99%

Effects of Fumed and Mesoporous Silica Nanoparticles on the Properties of Sylgard 184 Polydimethylsiloxane

Liu

Zong

et al. 2015

Micromachines

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“…The biocompatibility of these materials has been extensively validated in various biomedical applications (20,21). The sufficient gas permeation of the silicone elastomer enables oxygen supply for the bacteria (22)(23)(24).…”

Section: Resultsmentioning

confidence: 99%

Stretchable living materials and devices with hydrogel–elastomer hybrids hosting programmed cells

Liu

Tang

Tham

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

235

228

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Living systems, such as bacteria, yeasts, and mammalian cells, can be genetically programmed with synthetic circuits that execute sensing, computing, memory, and response functions. Integrating these functional living components into materials and devices will provide powerful tools for scientific research and enable new technological applications. However, it has been a grand challenge to maintain the viability, functionality, and safety of living components in freestanding materials and devices, which frequently undergo deformations during applications. Here, we report the design of a set of living materials and devices based on stretchable, robust, and biocompatible hydrogel-elastomer hybrids that host various types of genetically engineered bacterial cells. The hydrogel provides sustainable supplies of water and nutrients, and the elastomer is air-permeable, maintaining long-term viability and functionality of the encapsulated cells. Communication between different bacterial strains and with the environment is achieved via diffusion of molecules in the hydrogel. The high stretchability and robustness of the hydrogel-elastomer hybrids prevent leakage of cells from the living materials and devices, even under large deformations. We show functions and applications of stretchable living sensors that are responsive to multiple chemicals in a variety of form factors, including skin patches and gloves-based sensors. We further develop a quantitative model that couples transportation of signaling molecules and cellular response to aid the design of future living materials and devices. hydrogels | synthetic biology | genetically engineered bacteria | biochemical sensors | wearable devices G enetically engineered cells enabled by synthetic biology have accomplished multiple programmable functions, including sensing (1), responding (2), computing (3), and recording (4). Powered by this emerging capability to program cells into living computers (1-6), the integration of genetically encoded cells into freestanding materials and devices will not only provide new tools for scientific research but also, lead to unprecedented technological applications (7). However, the development of such living materials and devices has been significantly hampered by the demanding requirements for maintaining viable and functional cells in materials and devices plus biosafety concerns toward the release of genetically modified organisms into environments. For example, gene networks embedded in paper matrices have been used for low-cost rapid viruses detection and protein manufacturing (1). However, their gene networks are based on freeze-dried extracts from genetically engineered cells to operate, partially because the paper substrates cannot sustain long-term viability and functionality of living cells or prevent their leakage. As another example, by seeding cardiomyocytes on thin elastomer films, biohybrid devices have been developed as soft actuators (8) and biomimetic robots (9). However, because the cells are not protected or isolated...

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“…40 It is important to modify the PDMS surface property to allow for liquid perfusion as well as to enhance cell adhesion. 41 There are many ways of surface treatment on PDMS, 42,43 among which oxygen or air plasma is the easiest and most commonly used method.…”

Section: B Selective Plasma Treatmentmentioning

confidence: 99%

A microfluidic co-culture system to monitor tumor-stromal interactions on a chip

et al. 2014

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The living cells are arranged in a complex natural environment wherein they interact with extracellular matrix and other neighboring cells. Cell-cell interactions, especially those between distinct phenotypes, have attracted particular interest due to the significant physiological relevance they can reveal for both fundamental and applied biomedical research. To study cell-cell interactions, it is necessary to develop co-culture systems, where different cell types can be cultured within the same confined space. Although the current advancement in lab-on-a-chip technology has allowed the creation of in vitro models to mimic the complexity of in vivo environment, it is still rather challenging to create such co-culture systems for easy control of different colonies of cells. In this paper, we have demonstrated a straightforward method for the development of an on-chip co-culture system. It involves a series of steps to selectively change the surface property for discriminative cell seeding and to induce cellular interaction in a co-culture region. Bone marrow stromal cells (HS5) and a liver tumor cell line (HuH7) have been used to demonstrate this co-culture model. The cell migration and cellular interaction have been analyzed using microscopy and biochemical assays. This co-culture system could be used as a disease model to obtain biological insight of pathological progression, as well as a tool to evaluate the efficacy of different drugs for pharmaceutical studies. V C 2014 AIP Publishing LLC. [http://dx

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Compatibility of Mammalian Cells on Surfaces of Poly(dimethylsiloxane)

Cited by 349 publications

References 62 publications

Effects of Fumed and Mesoporous Silica Nanoparticles on the Properties of Sylgard 184 Polydimethylsiloxane

Effects of Fumed and Mesoporous Silica Nanoparticles on the Properties of Sylgard 184 Polydimethylsiloxane

Stretchable living materials and devices with hydrogel–elastomer hybrids hosting programmed cells

A microfluidic co-culture system to monitor tumor-stromal interactions on a chip

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