Compatible and Incompatible Mycorrhizal Fungi With Seeds of Dendrobium Species: The Colonization Process and Effects of Coculture on Germination and Seedling Development

Ma, Guang-Hui; Chen, Xianggui; Selosse, Marc‐André; Gao, Jiang-Yun

doi:10.3389/fpls.2022.823794

Cited by 6 publications

(4 citation statements)

References 53 publications

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“…The two fungal strains LQ and TPYD-2 were used together in the TP-(LQ+TPYD-2) treatment, but the percentages of seedlings at different time points after sowing were all significantly lower than those in the TP-LQ treatment ( Figure 2 ). The results are consistent with those of previous studies, in which fungal cocultures have been observed to result in significantly lower seed germination, protocorm formation and seedling development than monocultures ( Sharma et al., 2003 ; Ma et al., 2022 ). Such reduced efficiency as a result of functional redundancy between fungal species was also found in arbuscular mycorrhizal fungi and explained as induced competition ( Thonar et al., 2014 ; Gosling et al., 2016 ; Crossay et al., 2019 ).…”

Section: Discussionsupporting

confidence: 93%

“…Such reduced efficiency as a result of functional redundancy between fungal species was also found in arbuscular mycorrhizal fungi and explained as induced competition ( Thonar et al., 2014 ; Gosling et al., 2016 ; Crossay et al., 2019 ). The occurrence of antagonism or competition between mycorrhizal fungi has been suggested in D. officinale ( Ma et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

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A novel method to produce massive seedlings via symbiotic seed germination in orchids

Yang

Li²,

Gao³

2023

Front. Plant Sci.

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Orchids produce large numbers of dust-like seeds that rely heavily on orchid mycorrhizal fungi (OMFs) for germination. Using OMFs to facilitate orchid proliferation is considered an effective method for orchid conservation but still presents challenges in practice. In this study, orchid seed-fungus complexes, in which orchid seeds and fungal mycelia were embedded together to form granules, were developed as platforms to facilitate seed germination and seedling production. Overall, seedlings were produced by seed-fungus complexes for five orchid species with large variations in the percentages of seedlings produced among species/treatments. For the different fungal treatments in Dendrobium officinale, Sebacinales LQ performed much better than the other fungal strains. At 90 days after sowing, 75.8±2.6% seedlings were produced in the LQ treatment, which was significantly higher than in the Tulasnella sp. JM (22.0±3.0%) and Tulasnella sp. TPYD-2 (5.3±1.0%) treatments, as well as in the LQ and TPYD-2 cocultured treatment (40.4±3.2%), while no seedlings were formed in the Tulasnella sp. SSCDO-5 or control treatments. For the other four orchid species, only one compatible fungus for each species was used, and the percentages of seedlings in epiphytic Dendrobium devonianum (67.2±2.9%) and D. nobile (38.9±2.8%) were much higher than those in terrestrial Paphiopedilum spicerianum (2.9±1.1%) and Arundina graminifolia (6.7±2.1%) at 90 days after sowing. Adding 1% polymer water-absorbent resin to the seed-fungus complexes of D. officinale seeds with fungal strain Sebacinales LQ significantly increased seedling formation, while other additional substances showed negative effects on seedling formation. For the storage of seed-fungus complexes, it is recommended to store the seed-fungus complexes in valve bags at room temperature for a short time and at a low temperature of 4°C for no more than 30 days. As a platform for symbiotic seed germination, the seed-fungus complex can facilitate seed germination, produce seedlings and support subsequent seedling growth, and its seedling productivity depends on seed germination characteristics, seed viability, and the efficiency of fungi. Seed-fungus complexes have great potential to be used as propagules in orchid conservation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

A novel method to produce massive seedlings via symbiotic seed germination in orchids

Yang

Li²,

Gao³

2023

Front. Plant Sci.

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“…FDd1 treatment, seeds started to germinate (Stage 1) at 42 days after incubation and formed protocorms (Stage 2) 52 days after incubation; moreover, no seedlings developed until 62 days after incubation. Although hyphae were observed congregated at the basal end outside of seeds at the beginning of fungal incubation, FDd1 could not persistently colonize seeds of D. officinale and form These results strongly suggested that the pattern of hyphal colonization of seeds was well matched with the morphological differentiation of seed germination and seedling development, and compatible fungi could persistently colonize seeds and quickly promote the conversion of germinated seeds into seedlings [24].…”

Section: Discussionmentioning

confidence: 88%

“…During seed symbiotic germination, fungal hyphae grow into orchid tissues through the suspensor cells of seeds and form pelotons within cortical cells [22,23]. Compatible fungi can persistently colonize orchid seeds and quickly promote the conversion of germinated seeds into seedlings, while incompatible fungi do not persistently colonize seeds or further support seedling development [24].…”

Section: Introductionmentioning

confidence: 99%

What role does the seed coat play during symbiotic seed germination in orchids: an experimental approach with Dendrobium officinale

Chen

et al. 2022

BMC Plant Biol

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Background Orchids require specific mycorrhizal associations for seed germination. During symbiotic germination, the seed coat is the first point of fungal attachment, and whether the seed coat plays a role in the identification of compatible and incompatible fungi is unclear. Here, we compared the effects of compatible and incompatible fungi on seed germination, protocorm formation, seedling development, and colonization patterns in Dendrobium officinale; additionally, two experimental approaches, seeds pretreated with NaClO to change the permeability of the seed coat and fungi incubated with in vitro-produced protocorms, were used to assess the role of seed coat played during symbiotic seed germination. Results The two compatible fungi, Tulasnella sp. TPYD-2 and Serendipita indica PI could quickly promote D. officinale seed germination to the seedling stage. Sixty-two days after incubation, 67.8 ± 5.23% of seeds developed into seedlings with two leaves in the PI treatment, which was significantly higher than that in the TPYD-2 treatment (37.1 ± 3.55%), and massive pelotons formed inside the basal cells of the protocorm or seedlings in both compatible fungi treatments. In contrast, the incompatible fungus Tulasnella sp. FDd1 did not promote seed germination up to seedlings at 62 days after incubation, and only a few pelotons were occasionally observed inside the protocorms. NaClO seed pretreatment improved seed germination under all three fungal treatments but did not improve seed colonization or promote seedling formation by incompatible fungi. Without the seed coat barrier, the colonization of in vitro-produced protocorms by TPYD-2 and PI was slowed, postponing protocorm development and seedling formation compared to those in intact seeds incubated with the same fungi. Moreover, the incompatible fungus FDd1 was still unable to colonize in vitro-produced protocorms and promote seedling formation. Conclusions Compatible fungi could quickly promote seed germination up to the seedling stage accompanied by hyphal colonization of seeds and formation of many pelotons inside cells, while incompatible fungi could not continuously colonize seeds and form enough protocorms to support D. officinale seedling development. The improvement of seed germination by seed pretreatment may result from improving the seed coat hydrophilicity and permeability, but seed pretreatment cannot change the compatibility of a fungus with an orchid. Without a seed coat, the incompatible fungus FDd1 still cannot colonize in vitro-produced protocorms or support seedling development. These results suggest that seed coats are not involved in symbiotic germination in D. officinale.

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A simple method for in vitro growth stimulation of tomato plantlets (Solanum lycopersicum) through supplementation of Rhizophagus irregularis MUCL43194 spores in the MS culture medium

Hoang

Thao

Minh

et al. 2023

Plant Cell Tiss Organ Cult

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Compatible and Incompatible Mycorrhizal Fungi With Seeds of Dendrobium Species: The Colonization Process and Effects of Coculture on Germination and Seedling Development

Cited by 6 publications

References 53 publications

A novel method to produce massive seedlings via symbiotic seed germination in orchids

A novel method to produce massive seedlings via symbiotic seed germination in orchids

What role does the seed coat play during symbiotic seed germination in orchids: an experimental approach with Dendrobium officinale

A simple method for in vitro growth stimulation of tomato plantlets (Solanum lycopersicum) through supplementation of Rhizophagus irregularis MUCL43194 spores in the MS culture medium

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