Compatible validated spectrofluorimetric and spectrophotometric methods for determination of vildagliptin and saxagliptin by factorial design experiments

“…This design was carried out to identify different parameters that could significantly affect the reaction of the proposed method. [32,33] The influence of factors affecting complex formation could be Table 1.…”

Section: Optimization Of Methods Conditions By Factorial Designmentioning

confidence: 99%

New spectrofluorimetric analysis of dapagliflozin after derivatization with NBD‐Cl in human plasma using factorial design experiments

et al. 2019

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A new validated spectrofluorimetric method was proposed for dapagliflozin (DGF) analysis in bulk, plexin its commercially available tablets and in spiked human plasma.The proposed spectrofluorimetric method depended on the formation of a fluorescent complex soluble in organic liquids by a substitution reaction between 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) reagent and DGF in aqueous buffered solution at pH 7. The fluorescence intensity was measured at 522 nm after excitation at 453 nm. The high selectivity of the proposed method allowed analysis of DGF in dosage form and human plasma samples with average recovery values of 99.84 ± 1.38% and 98.71 ± 1.80%, respectively, without any interference from matrix components. The calibration range was 50-1000 ng ml −1 . The limit of detection (LOD) and limit of quantitation (LOQ) were 14.24 ng ml −1 and 43.14 ng ml −1 , respectively. The estimated relative standard deviation values were lower than 2.0%, this showed the excellent precision at both levels. Factorial design was used to get the optimum method conditions for the analysis of the resulting DGF fluorescence complex in different matrices. The proposed method could be used in routine analysis of DGF in quality control laboratories. Also, it could be used to assay DGF in human plasma and be applied for pharmacokinetic investigation of DGF.

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“…It could be taken alone or with other antidiabetic medications 1 . Various methods have been published for the estimation of VIL using UV spectrophotometric 2,3 , spectrofluorimetric 4,5 , liquid chromatographic methods [6][7][8][9][10][11] and voltammetric method 12 .…”

Section: Introductionmentioning

confidence: 99%

Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma

Mohamed

Alamin

Tubar

2022

Journal of Advanced Pharmacy Research

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Compatible validated spectrofluorimetric and spectrophotometric methods for determination of vildagliptin and saxagliptin by factorial design experiments

Cited by 23 publications

References 40 publications

Determination of midodrine hydrochloride via Hantzsch condensation reaction: a factorial design based spectrophotometric approach

Determination of midodrine hydrochloride via Hantzsch condensation reaction: a factorial design based spectrophotometric approach

New spectrofluorimetric analysis of dapagliflozin after derivatization with NBD‐Cl in human plasma using factorial design experiments

Green Spectrofluorimetric Technique for the Determination of Vildagliptin Through Quenching Lanthanide Luminescence in Pure, Pharmaceutical Dosage Form and Spiked Plasma

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