Competence and Sporulation in Bacillus subtilis

Gupta, Rani; Gupta, Namita

doi:10.1007/978-981-16-0723-3_25

Cited by 2 publications

(3 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…B. subtilis cells respond to a range of stress by spore formation. This process involves up to 500 genes [ 49 , 50 , 51 , 52 , 53 ] that tend to use minor codons including the CGA codon [ 54 , 55 ]. We then examined the effect of yaaJ deletion on heat-resistant spore formation.…”

Section: Resultsmentioning

confidence: 99%

“…KUB10 showed only a slight decrease in sporulation efficiency ( Table 2 ) suggesting that inosine modification of tRNA Arg is not essential for the expression of sporulation genes. On the path toward sporulation, some of the B. subtilis cells become naturally competent and thus have the opportunity to adapt to their environment [ 51 , 53 ]. We found that the competency of KUB10 decreased by 100-fold ( Table 3 ), indicating the significance of the yaaJ gene.…”

Section: Resultsmentioning

confidence: 99%

“…subtilis cells respond to a range of stress by spore formation. This process involves up to 500 genes [49][50][51][52][53] that tend to use minor codons including the CGA codon [54,55].…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

yaaJ, the tRNA-Specific Adenosine Deaminase, Is Dispensable in Bacillus subtilis

Soma,

Kubota,

Tomoe

et al. 2023

Genes

View full text Add to dashboard Cite

Post-transcriptional modifications of tRNA are crucial for their core function. The inosine (I; 6-deaminated adenosine) at the first position in the anticodon of tRNAArg(ICG) modulates the decoding capability and is generally considered essential for reading CGU, CGC, and CGA codons in eubacteria. We report here that the Bacillus subtilis yaaJ gene encodes tRNA-specific adenosine deaminase and is non-essential for viability. A β−galactosidase reporter assay revealed that the translational activity of CGN codons was not impaired in the yaaJ-deletion mutant. Furthermore, tRNAArg(CCG) responsible for decoding the CGG codon was dispensable, even in the presence or absence of yaaJ. These results strongly suggest that tRNAArg with either the anticodon ICG or ACG has an intrinsic ability to recognize all four CGN codons, providing a fundamental concept of non-canonical wobbling mediated by adenosine and inosine nucleotides in the anticodon. This is the first example of the four-way wobbling by inosine nucleotide in bacterial cells. On the other hand, the absence of inosine modification induced +1 frameshifting, especially at the CGA codon. Additionally, the yaaJ deletion affected growth and competency. Therefore, the inosine modification is beneficial for translational fidelity and proper growth-phase control, and that is why yaaJ has been actually conserved in B. subtilis.

show abstract