Competence for neural crest induction is controlled by hydrostatic pressure through Yap

Abstract

Embryonic induction is a key mechanism in development that corresponds to an interaction between a signalling and a responding tissue, causing a change in the direction of differentiation by the responding tissue. Considerable progress has been achieved in identifying inductive signals, yet how tissues control their responsiveness to these signals, known as competence, remains poorly understood. While the role of molecular signals in competence has been studied, how tissue mechanics influence competence remains unexplored. Here we investigate the role of hydrostatic pressure in controlling competence in neural crest cells, an embryonic cell population. We show that neural crest competence decreases concomitantly with an increase in the hydrostatic pressure of the blastocoel, an embryonic cavity in contact with the prospective neural crest. By manipulating hydrostatic pressure in vivo, we show that this increase leads to the inhibition of Yap signalling and impairs Wnt activation in the responding tissue, which would be required for neural crest induction. We further show that hydrostatic pressure controls neural crest induction in amphibian and mouse embryos and in human cells, suggesting a conserved mechanism across vertebrates. Our work sets out how tissue mechanics can interplay with signalling pathways to regulate embryonic competence.

Conserved signals orchestrate self-organization and symmetry breaking of bi-layered epithelia during development and regeneration

“…In conclusion, in agreement with very recent studies (Alasaadi et al, 2024;Shroff et al, 2024), we show that tissue architecture and geometry can regulate molecular pathways to drive the establishment of fate patterns, and that YAP activity represents a central player in coordinating the temporal and spatial dimensions during tissue morphogenesis and regeneration.…”