Competition between<i>Rhizobium</i>strain NGR234 and<i>Bradyrhizobium</i>strain CP283 for nodulation in Siratro investigated with the GUS reporter gene

Khan, Monowar Karim; Prasad, B. G.; Akao, Shoichiro

doi:10.1080/00380768.1999.10414331

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…Although one can never be certain that these chromosomal insertions (mutations) are innocuous, the results presented here strongly suggest that this is the case. Other workers, employing these same genetic markers for pseudomonads (Hattemer-Frey et al, 1990;Kluepfel et al, 1991;Parke et al, 1992;Hartel et al, 1994) and rhizobia (Wilson et al, 1991;Kang et al, 1997;Khan et al, 1999) have presented evidence affirming this suggestion.…”

Section: Discussionmentioning

confidence: 87%

Survival of a Genetically Modified Root‐Colonizing Pseudomonad and Rhizobium Strain in an Acidic Soil

Staley¹,

Bräuer

2006

Soil Science Soc of Amer J

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Maintaining threshold populations of inoculum microorganisms in the soil environment is important for such practical applications as biocontrol, plant growth-promotion, bioremediation, and nodulation. However, because of both technical and labor constraints in monitoring bacterial viability in nonsterile soils, few studies have reported on survival kinetics, particularly in relation to subtle alterations in soil acidity-related factors. A genetically modified strain Pseudomonas putida R20/lacZY or Rhizobium leguminosarum bv. trifolii 162S7a/ gusA was introduced into conditioned, nonsterile Gilpin (fine loamy, mixed mesic, Typic Hapludult) silt loam soil, limed at four low levels (pH w 4.71, 4.81, 4.92, and 4.99) or derivative soil solutions with highly correlated (R 2 $ 0.81) chemical properties. Immediate declines in viability of both strains were found in all soils, reaching 0.1 to 1% initial colony-forming unit (CFU) g 21 soil in 35 h for P. putida and in 68 h for R. leguminosarum bv. trifolii. Death rate constants (k d) for both strains were directly related to lime level (soil pH), although differences were not significant (P. 0.05) for the rhizobium. Use of soil solutions gave similar responses for both strains, but over much shorter incubation times. As with soils, k d values for both strains in soil solutions were directly related to lime level (solution pH). In both soil and soil solution experiments, survival (k d) was negatively correlated (R 2 $ 20.914) with pH and basic cation (Ca and Mg), and positively correlated (R 2 $ 0.933) with Al, concentrations. This relationship of viability to soil solution chemistry was broadly confirmed for both bacterial strains by use of fluorescent probes, suggesting increased cell membrane damage at lower pHs. These results demonstrate not only the alternative utility of using soil solutions, rather than nonsterile soils, for bacterial viability assessments, but also the positive effect of low-level liming (|0.28 pH unit increase) on survival of beneficial root-colonizing bacteria in acidic soils.

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Section: Discussionmentioning

confidence: 87%

Survival of a Genetically Modified Root‐Colonizing Pseudomonad and Rhizobium Strain in an Acidic Soil

Staley¹,

Bräuer

2006

Soil Science Soc of Amer J

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“…The GUS marker gene system developed by Wilson [44] has been applied successfully for nodule competitiveness studies of rhizobia in short‐term experiments in greenhouse conditions [45–47]. However, only a limited number of studies have been conducted in field conditions.…”

Section: Discussionmentioning

confidence: 99%

Persistence, population dynamics and competitiveness for nodulation of marker gene-tagged Rhizobium galegae strains in field lysimeters in the boreal climatic zone

Pitkäjärvi

Rásänen

Langenskiöld

et al. 2003

FEMS Microbiology Ecology

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A non-indigenous wild-type strain Rhizobium galegae HAMBI 540, which specifically nodulates perennial goat's rue (Galega orientalis), and its marker gene-tagged derivatives R. galegae HAMBI 2363(luc), R. galegae HAMBI 2368(gusA21) and R. galegae HAMBI 2364(gusA30) were used to evaluate the persistence, population dynamics and competitiveness for nodulation of rhizobia under field conditions in Finland. The lysimeters were filled with clean or diesel oil-polluted (3000 Wg g 31 ) agricultural soil. During the first 2 years of the field release luc-and gusA21-tagged strains could be effectively detected by cultivation, reinforced with colony polymerase chain reaction. The population densities remained relatively stable from 10 4 to 10 5 cfu g 31 dry soil from spring until late autumn. Replicate limiting dilution polymerase chain reaction analysis gave comparable results with cultivation with strain HAMBI 2363 until 49 weeks after inoculation. GUS activity of strain HAMBI 2368 could be stably detected in nodules and soil. On the other hand, luc activity weakened clearly in cold conditions along with decreased metabolic activity of rhizobia. The competitive ability for nodulation of the gusA30-tagged strain decreased slowly with time compared to the wild-type strain. Moderate soil pollution did not have significant effects on target bacteria or plant growth. Limited vertical movement of target bacteria outside the rhizosphere was detected from percolated water.

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“…For the detection of inoculated bacteria in the environment, new techniques that introduce a specific marker gene have been developed and widely used. In particular, the gusA gene encoding β‐glucuronidase (GUS) enzyme is well utilized as an effective and transmitting marker (Khan et al. 1999; Sessitsch et al.…”

Section: Methodsmentioning

confidence: 99%

Proliferation of inoculatedRhizobiumand indigenous microbes around the periphery of pre-rooting common beanPhaseolus vulgarisL. seeds sown in non-sterile soils

Kato

Hirata

Sekimoto

et al. 2007

Soil Science and Plant Nutrition

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The proliferation of inoculated gusA-marked (encoding β-glucuronidase) Rhizobium and indigenous microbes around the periphery of common bean (Phaseolus vulgaris L. cv. Hokkaikintoki, Himetebou and Kurodanekinugasa) seeds sown in non-sterile Japanese soils (Andosol, Gray Lowland soil and Sandy Regosol) were investigated. The number of gusA-marked Rhizobium cells inoculated increased 24 h after sowing. The population of gusA-marked Rhizobium and indigenous bacteria tended to be smaller around seeds of Hokkaikintoki compared with the other cultivars. The population of gusA-marked Rhizobium and indigenous bacteria also tended to be smaller in Andosol than in the other soils. The proliferative response of indigenous fungi was weaker than that of gusA-marked Rhizobium or indigenous bacteria. These results indicated that Rhizobium inoculated around the periphery of common bean seeds could proliferate efficiently under the existence of indigenous microbes and that the intensity of proliferation was influenced by the type of common bean cultivar and soil type.

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Competition betweenRhizobiumstrain NGR234 andBradyrhizobiumstrain CP283 for nodulation in Siratro investigated with the GUS reporter gene

Cited by 6 publications

References 31 publications

Survival of a Genetically Modified Root‐Colonizing Pseudomonad and Rhizobium Strain in an Acidic Soil

Survival of a Genetically Modified Root‐Colonizing Pseudomonad and Rhizobium Strain in an Acidic Soil

Persistence, population dynamics and competitiveness for nodulation of marker gene-tagged Rhizobium galegae strains in field lysimeters in the boreal climatic zone

Proliferation of inoculatedRhizobiumand indigenous microbes around the periphery of pre-rooting common beanPhaseolus vulgarisL. seeds sown in non-sterile soils

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