Competition between two microbial populations in a sequencing fed‐batch reactor: Theory, experimental verification, and implications for waste treatment applications

Dikshitulu, Sitaram; Baltzis, Basil C.; Lewandowski, G. A.; Pavlou, Stavros

doi:10.1002/bit.260420513

Cited by 76 publications

(36 citation statements)

References 40 publications

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“…The values of parameters were listed in Table 1. All these values were within the range reported in the literatures depending on cell type and culture environments (Dikshitulu et al 1993;Kumar et al 2005). The value of the root mean square of the residual at these parameters was small (0.035) for phenol biodegradation, indicating that the regression parameters agreed well with the experimental data.…”

Section: Single Substrate Intrinsic Kineticssupporting

confidence: 63%

Biodegradation of phenol and 4-chlorophenol by the yeast Candida tropicalis

Jiang

Wen

Li³

et al. 2007

Biodegradation

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Biodegradation of phenol and 4-chlorophenol (4-cp) using a pure culture of Candida tropicalis was studied. The results showed that C. tropicalis could degrade 2,000 mg l(-1) phenol alone and 350 mg l(-1) 4-cp alone within 66 and 55 h, respectively. The capacity of the strain to degrade phenol was obviously higher than that to degrade 4-cp. In the dual-substrate system, 4-cp intensely inhibited phenol biodegradation. Phenol beyond 800 mg l(-1) could not be degraded in the presence of 350 mg l(-1) 4-cp. Comparatively, low-concentration phenol from 100 to 600 mg l(-1) supplied a sole carbon and energy source for C. tropicalis in the initial phase of biodegradation and accelerated the assimilation of 4-cp, which resulted in the fact that 4-cp biodegradation velocity was higher than that without phenol. And the capacity of C. tropicalis to degrade 4-cp was increased up to 420 mg l(-1) with the presence of 100-160 mg l(-1) phenol. In addition, the intrinsic kinetics of cell growth and substrate degradation were investigated with phenol and 4-cp as single and mixed substrates in batch cultures. The results illustrated that the models proposed adequately described the dynamic behaviors of biodegradation by C. tropicalis.

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Section: Single Substrate Intrinsic Kineticssupporting

confidence: 63%

Biodegradation of phenol and 4-chlorophenol by the yeast Candida tropicalis

Jiang

Wen

Li³

et al. 2007

Biodegradation

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“…We assume that Y X /S = 0.7 for Pseudomonas sp. grown in phenol (Dikshitulu et al, 1993;Erhan et al, 2004). Various experimentally determined values of Y X /S are reported in the literature ranging from 0.52-0.94 (Reardon et al, 2000).…”

Section: Theoretical Backgroundmentioning

confidence: 98%

Bioprocess intensification in flow‐through monolithic microbioreactors with immobilized bacteria

Akay

Erhan

Keskinler

2005

Biotech & Bioengineering

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Microporous polymers (with porosity up to 90%) with a well-prescribed internal microstructure were prepared in monolithic form to construct a flow-through microbioreactor in which phenol-degrading bacteria, Pseudomonas syringae, was immobilized. Initially, bacteria was forced seeded within the pores and subsequently allowed to proliferate followed by acclimatization and phenol degradation at various initial substrate concentrations and flow rates. Two types of microporous polymer were used as the monolithic support. These polymers differ with respect to their pore and interconnect sizes, macroscopic surface area for bacterial support, and phase volume. Polymer with a nominal pore size of 100 microm with phase volume of 90% (with highly open pore structure) yielded reduced bacterial proliferation, while the polymer with nominal pore size of 25 microm with phase volume of 85% (with small interconnect size and large pore area for bacterial adhesion) yielded monolayer bacterial proliferation. Bacteria within the 25 microm polymer support remained monolayered, without any apparent production of extracellular matrix during the 30-day continuous experimental period. The microbioreactor performance was characterized in terms of volumetric utilization rate and compared with the published data, including the case where the same bacteria was immobilized on the surface of microporous polymer beads and used in a packed bed during continuous degradation of phenol. It is shown that at similar initial substrate concentration, the volumetric utilization in the microreactor is at least 20-fold more efficient than the packed bed, depending on the flow rate of the substrate solution. The concentration of the bacteria within the pores of the microreactor decreases from 2.25 cells per microm2 on the top surface to about 0.4 cells per microm2 within 3 mm reactor depth. If the bacteria-depleted part of the microreactor is disregarded, the volumetric utilization increases by a factor of 30-fold compared with the packed bed. This efficiency increase is attributed to the reduction of diffusion path for the substrate and nutrients and enhanced availability of the bacteria for bioconversion in the absence of biofilm formation as well as the presence of flow over the surface of the monolayer bacteria.

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“…In spite of the competitive exclusion having been verified experimentally [8], [13], [36], the uniform persistence is observed in several ecosystems. This duality has stimulated a considerable amount of work in order to be explained [30], [31].…”

Section: Introductionmentioning

confidence: 99%

Global stability for a model of competition in the chemostat with microbial inputs

Robledo¹,

Grognard²,

Gouzé³

2012

Nonlinear Analysis: Real World Applications

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We propose a model of competition of n species in a chemostat, with constant input of some species. We mainly emphasize the case that can lead to coexistence in the chemostat in a non-trivial way, i.e., where the n−1 less competitive species are in the input. We prove that if the inputs satisfy a constraint, the coexistence between the species is obtained in the form of a globally asymptotically stable (GAS) positive equilibrium, while a GAS equilibrium without the dominant species is achieved if the constraint is not satisfied. This work is round up with a thorough study of all the situations that can arise when having an arbitrary number of species in the chemostat inputs; this always results in a GAS equilibrium that either does or does not encompass one of the species that is not present in the input

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Competition between two microbial populations in a sequencing fed‐batch reactor: Theory, experimental verification, and implications for waste treatment applications

Cited by 76 publications

References 40 publications

Biodegradation of phenol and 4-chlorophenol by the yeast Candida tropicalis

Biodegradation of phenol and 4-chlorophenol by the yeast Candida tropicalis

Bioprocess intensification in flow‐through monolithic microbioreactors with immobilized bacteria

Global stability for a model of competition in the chemostat with microbial inputs

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