Competition for H2 between sulfate reduction and dechlorination in butyrate-fed anaerobic cultures

Aulenta, Federico; Beccari, M.; Majone, Mauro; Papini, Marco Petrangeli; Tandoi, Valter

doi:10.1016/j.procbio.2007.11.006

Cited by 64 publications

(49 citation statements)

References 29 publications

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“…Conversely, sulfate did not affect dechlorination when rapid fermentation of lactate resulted in accumulation of hydrogen to levels Ͼ100 nM. Aulenta et al (27) reported that 3.7 mM sulfate adversely affected the rate of reductive dechlorination of an enriched dechlorinating community. These inconsistent findings make it difficult to understand potential mechanisms of sulfate inhibition and complicate the interpretation of bioremediation field data.…”

Section: Discussionmentioning

confidence: 99%

“…There are a limited number of laboratory studies with detailed information on the effects of sulfate on dechlorination (21,(26)(27)(28). In addition, some conflicting results due to sulfate addition, ranging from enhanced dechlorination (27,29,30) to inhibited or incomplete dechlorination (15,27,29,31,32), as well as no observed effect on dechlorination (16,25), have been reported over the past decade.…”

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confidence: 99%

“…In addition, some conflicting results due to sulfate addition, ranging from enhanced dechlorination (27,29,30) to inhibited or incomplete dechlorination (15,27,29,31,32), as well as no observed effect on dechlorination (16,25), have been reported over the past decade. A review of published field data from TCE-contaminated sites with sulfate concentrations ranging from 39 to 4,800 mg liter Ϫ1 reported the overall trend that as sulfate concentrations increased, dechlorination reactions became incomplete or delayed (26).…”

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confidence: 99%

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Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Mao

Polasko

Alvarez‐Cohen

2017

Appl Environ Microbiol

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In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit the growth or metabolism of pure cultures of the dechlorinator Dehalococcoides mccartyi 195, the sulfate reducer Desulfovibrio vulgaris Hildenborough, or the syntroph Syntrophomonas wolfei. In contrast, sulfide at 5 mM exhibited inhibitory effects on growth of the sulfate reducer and the syntroph, as well as on both dechlorination and growth rates of D. mccartyi. Transcriptomic analysis of D. mccartyi 195 revealed that genes encoding ATP synthase, biosynthesis, and Hym hydrogenase were downregulated during sulfide inhibition, whereas genes encoding metal-containing enzymes involved in energy metabolism were upregulated even though the activity of those enzymes (hydrogenases) was inhibited. When the electron acceptor (trichloroethene) was limiting and an electron donor (lactate) was provided in excess to cocultures and enrichments, high sulfate concentrations (5 mM) inhibited reductive dechlorination due to the toxicity of generated sulfide. The initial cell ratio of sulfate reducers to D. mccartyi (1:3, 1:1, or 3:1) did not affect the dechlorination performance in the presence of sulfate (2 and 5 mM). In contrast, under electron donor limitation, dechlorination was not affected by sulfate amendments due to low sulfide production, demonstrating that D. mccartyi can function effectively in anaerobic microbial communities containing moderate sulfate concentrations (5 mM), likely due to its ability to outcompete other hydrogen-consuming bacteria and archaea.IMPORTANCE Sulfate is common in subsurface environments and has been reported as a cocontaminant with chlorinated solvents at various concentrations. Inconsistent results for the effects of sulfate inhibition on the performance of dechlorination enrichment cultures have been reported in the literature. These inconsistent findings make it difficult to understand potential mechanisms of sulfate inhibition and complicate the interpretation of bioremediation field data. In order to elucidate interactions between sulfate reduction and reductive dechlorination, this study systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. This study provides a more fundamental understanding of the competition mechanisms between reductive dechlorination by Dehalococcoides mccartyi and sulfate reduction during the bioremediation process. It also provides insights on the significance of sulfate concentrations on reductive dechlorination under electron donor/acceptor-limiting conditions during in situ bioremediation applications. For example, at a trichloroethene-contaminated site with a high sulfate concentration, proper...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Mao

Polasko

Alvarez‐Cohen

2017

Appl Environ Microbiol

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“…The dehalorespiration bacteria compete with hydrogenotrophs, such as sulphate-reducers, nitrate-reducers, methanogens, acetogens, and other reducers [46,47]. Except hydrogen, other electron donors also can be used for reductive dehalogenation, ex.…”

Section: Anaerobic Reductive Dehalogenationmentioning

confidence: 99%

Microbial Degradation of Some Halogenated Compounds: Biochemical and Molecular Features

Peng¹,

Shih²

2013

Biodegradation of Hazardous and Special Products

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“…Provided that appropriate environmental conditions exist, chlorinated aliphatic and aromatic hydrocarbons undergo reductive dechlorination in sulfatereducing environments with different electron donors (Alder et al 1993;Aulenta et al 2008;Bagley and Gossett 1990;Cabirol et al 1998;De Best et al 1997a, b;Häggblom and Young 1990;Kennedy et al 2006;Ndon et al 2000;Palekar et al 2003;Pavlostathis and Zhuang 1991;Prytula and Pavlostathis 1996;Sonier et al 1994). Degradation of chlorinated aromatic compounds coupled to sulfate reduction yields higher free energy than degradation coupled to methane production (Häggblom and Young 1995).…”

Section: Introductionmentioning

confidence: 99%

Influence of sulfate reduction on the microbial dechlorination of pentachloroaniline in a mixed anaerobic culture

Ismail

Pavlostathis

2009

Biodegradation

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The reductive dechlorination of pentachloroaniline (PCA) was investigated in the absence and presence of sulfate in batch assays using a PCA-dechlorinating mixed anaerobic culture with methanol as the external electron donor at neutral pH and 22 degrees C. PCA at an initial concentration of 7.8 microM was sequentially dechlorinated to dichlorinated anilines in the sulfate-free culture and the culture amended with 300 mg sulfate-S/L. At an initial concentration of 890 mg sulfate-S/L, a higher sulfate reduction rate was achieved, but PCA dechlorination was not observed until the sulfate concentration dropped to about 100 mg S/L. The transient inhibition of PCA is attributed to competition between sulfate reducing and dechlorinating species for electron donor, more likely for H(2) resulting from methanol fermentation. A long-term (118 days) PCA dechlorination assay with the sulfate-amended culture, which included five feeding cycles, resulted in accumulation of both sulfide (886 mg S/L) and acetate (1,900 mg COD/L). Under these conditions, the sulfate reducers were inhibited, while the rate and pathway of PCA dechlorination were not affected. The results of this study show that the rate of sulfate reduction rather than the sulfate concentration alone dictates the outcome of the competition between sulfate reducers and either dechlorinators or methanogens. The findings of the present study have significant implications relative to the fate and transport of PCA and its dechlorination products in sulfate-laden subsurface systems.

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Competition for H2 between sulfate reduction and dechlorination in butyrate-fed anaerobic cultures

Cited by 64 publications

References 29 publications

Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities

Microbial Degradation of Some Halogenated Compounds: Biochemical and Molecular Features

Influence of sulfate reduction on the microbial dechlorination of pentachloroaniline in a mixed anaerobic culture

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