Competition for nitrate between denitrifying<i>Pseudomonas stutzeri</i>and nitrate ammonifying enterobacteria

Rehr, Bert; Klemme, Jobst-Heinrich

doi:10.1111/j.1574-6968.1989.tb03657.x

Cited by 34 publications

(14 citation statements)

References 21 publications

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“…Nevertheless, nitrate concentration in natural aquatic and terrestrial ecosystems is normally low (e.g., <100 μM, Table ), at which the boundaries of different competition outcomes changed dramatically (Figure ). Lab-scale competition studies often supply high concentrations of nitrate (>1000 μM, e.g., in refs , , ) at which the boundaries were rather stable and mainly defined by the stoichiometries of denitrification and DNRA (Figure ). The thresholds obtained from these high-nitrate environments closely resembled the results of our model.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Overall, the results highlight the impact of dilution rate on competition outcome, especially at low influent resource concentrations and/or at high dilution rates. In mixed pure cultures of DNRA bacteria (Citrobacter freundii) and denitrifiers (Pseudomonas stutzeri) competing for nitrate and lactate, stable coexistence was obtained at a low dilution rate (0.05 h –1 ), whereas DNRA bacteria started to be washed out at a dilution rate (0.1 h –1 ) much lower than its μ max (0.19 h –1 ) even under nitrate-limiting conditions, which would normally favor DNRA bacteria. The latter could be justified by the D C for coexistence (Figure A), above which DNRA could not coexist or outcompete denitrification.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Apart from energetics and kinetics, environmental conditions affect the biological nitrate partitioning as well. There are multiple studies suggesting that the competition depends on the dilution rate , and initial resource concentration . In addition, other studies conducted with a pure culture that encompasses a dual pathway showed that COD/N ratio alone was insufficient to explain pathway selection at low resource concentrations as the culture disproportionately utilizes DNRA rather than denitrification .…”

Section: Introductionmentioning

confidence: 99%

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Elucidating the Competition between Heterotrophic Denitrification and DNRA Using the Resource-Ratio Theory

Jia

Winkler

2020

Environ. Sci. Technol.

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Heterotrophic denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes competing for two shared resources, namely, nitrate and organic carbon (COD). Their competition has great implications for nitrogen loss, conservation, and greenhouse gas emissions. Nevertheless, a comprehensive and mechanistic understanding of the governing factors for this competition is still lacking. We applied the resource-ratio theory to study this competition and validated the theory with experimental data from continuous cultures reported in the literature. Based on this theory, we revealed that influent COD/N ratio alone was not sufficient to predict the competition outcome as the boundary values for different competition outcomes changed substantially with influent resource concentrations. The stoichiometry of the two processes was determinative for the boundaries, whereas the affinity for the shared resources (KS ), maximum specific growth rate (μmax) of the two species, and the dilution rate had significant impacts as well but mainly at low influent resource concentrations (e.g., <100 μM nitrate). The presented approach allows for a more comprehensive understanding of the parameters controlling microbial competition. The computational comparison between continuous and batch cultures could explain seemingly conflicting experimental results as to the impact of the COD/N ratio. The results also include testable hypotheses and tools for understanding and managing the fate of nitrate in ecosystems, which could also be applied more widely to other species competing for two shared resources.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Elucidating the Competition between Heterotrophic Denitrification and DNRA Using the Resource-Ratio Theory

Jia

Winkler

2020

Environ. Sci. Technol.

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“…A predictive understanding of how environmental perturbations influence the microbially-mediated nitrogen cycle has major implications for sustainable agriculture, wastewater treatment, and toxin remediation( 1, 2 ). Previous work has demonstrated linkages between changes in carbon composition and microbial community composition( 21 - 24 ), carbon composition and respiratory end-products( 5, 10, 53 ), or community composition and respiratory end-products( 6 ). However, few studies to date have examined the dynamics of nitrate respiring microbial communities using metagenomic sequencing ( 6 ), and rarely are the dynamics of genes, strains and respiratory traits systematically linked in a high-throughput format as we have in this study.…”

Section: Discussionmentioning

confidence: 99%

Selective carbon sources influence the end-products of microbial nitrate respiration

Carlson

Lui

Price

et al. 2019

Preprint

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AbstractRespiratory and catabolic pathways are differentially distributed in microbial genomes. Thus, specific carbon sources may favor different respiratory processes. We profiled the influence of 94 carbon sources on the end-products of nitrate respiration in microbial enrichment cultures from diverse terrestrial environments. We found that some carbon sources consistently favor dissimilatory nitrate reduction to ammonium (DNRA/nitrate ammonification) while other carbon sources favor nitrite accumulation or denitrification. For an enrichment culture from aquatic sediment, we sequenced the genomes of the most abundant strains, matched these genomes to 16S rDNA exact sequence variants (ESVs), and used 16S rDNA amplicon sequencing to track the differential enrichment of functionally distinct ESVs on different carbon sources. We found that changes in the abundances of strains with different genetic potentials for nitrite accumulation, DNRA or denitrification were correlated with the nitrite or ammonium concentrations in the enrichment cultures recovered on different carbon sources. Specifically, we found that either L-sorbose or D-cellobiose enriched for a Klebsiella nitrite accumulator, other sugars enriched for an Escherichia nitrate ammonifier, and citrate or formate enriched for a Pseudomonas denitrifier and a Sulfurospirillum nitrate ammonifier. Our results add important nuance to the current paradigm that higher concentrations of carbon will always favor DNRA over denitrification or nitrite accumulation, and we propose that, in some cases, carbon composition can be as important as carbon concentration in determining nitrate respiratory end-products. Furthermore, our approach can be extended to other environments and metabolisms to characterize how selective parameters influence microbial community composition, gene content and function.

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“…They are metabolic pluripotent organism with multiple functional genes, which can reduce organics, nitrates, sulfates and phosphates, and some species may be related to DNRA (Wu et al 2018). Clostridia, a fermentative bacteria, can reduce nitrate to nitrite and then decompose to ammonium (Rehr and Klemme 1989). This bacterium may play a role in the DNRA process.…”

Section: Dnra Functional Bacteria In Two Systemsmentioning

confidence: 99%

Role of Organic/Sulfide Ratios On DNRA Cultivation And The Microbial Community Structure of A Co-Driven Sequencing Biofilm Batch Reactor

Zhao

Zhang

et al. 2021

Preprint

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Denitrification and dissimilatory nitrate reduction to ammonium (DNRA), are two competing pathways in nitrate reducing process. In this study, a series of C/S ratios from 8:1 to 2:4 was investigated in a sequencing biofilm batch reactor (SBBR) to determine the role of reducers (sulfide and acetate) on their competition. The results showed the proportion of DNRA increased in high electron system, either in organic rich or in sulfide rich system. The highest DNRA ratio increased to 16.7% at the C/S ratio of 2:3. Excess electron donors, particularly sulfide, were favorable for DNRA in a limited nitrate environment. Moreover, a higher reductive environment (ORP <-400 mV) can be used as an indicator for the occurrence of DNRA. 16s RNA analysis demonstrated that Grobacter was the main functional bacteria of DNRA in the organic rich system, while Alphaproteobacteria and Desulfomicrobium were dominant DNRA bacteria in the sulfide rich system. DNRA cultivation could enrich nitrogen conversion pathways in conventional denitrification systems. This provides the great insight into nitrogen removal in high nitrogen containing sewage with low C/N.

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Competition for nitrate between denitrifyingPseudomonas stutzeriand nitrate ammonifying enterobacteria

Cited by 34 publications

References 21 publications

Elucidating the Competition between Heterotrophic Denitrification and DNRA Using the Resource-Ratio Theory

Elucidating the Competition between Heterotrophic Denitrification and DNRA Using the Resource-Ratio Theory

Selective carbon sources influence the end-products of microbial nitrate respiration

Role of Organic/Sulfide Ratios On DNRA Cultivation And The Microbial Community Structure of A Co-Driven Sequencing Biofilm Batch Reactor

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