Competition of three aggregated microbial species for four substrates in the rumen

Witten, Gareth; Richardson, F. D.

doi:10.1016/s0304-3800(02)00383-6

Cited by 7 publications

(3 citation statements)

References 27 publications

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“…Nguni faeces displayed lower hydrolytic and dehydrogenase activity compared to other ungulates, which could be due to lower proportions of hydrolase producers present and the different digestive system. These findings are consistent with previous reports in the literature suggesting that cellulolytic microorganisms when in competition with other specialists are present at low proportions (Witten and Richardson 2003 ). The different digestive systems present in ruminants (for example giraffe) and hindgut digesters such as zebra might result in different microbial communities.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms

Ndlela

Schmidt

2016

SpringerPlus

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This study assessed faecal matter from three indigenous South African herbivores—zebra, giraffe and impala—as a potential source for hydrolytically active aerobic and facultatively anaerobic bacteria. Herbivore droppings were collected freshly in a local nature reserve in Pietermaritzburg, South Africa. Soil samples adjacent to faecal collection sites and faeces from a domestic herbivore, the Nguni cow, were included as controls. Hydrolase and dehydrogenase activity in faecal matter and soil samples were measured by the fluorescein diacetate and the triphenyltetrazolium chloride assay. Viable counts and counts for amylase, cellulase, esterase and protease producers were established using plate count agar and solid media containing cellulose, skim milk, starch and Tween 80. Zebra droppings produced the highest hydrolase and dehydrogenase activity. Faecal matter of the three indigenous herbivores generally produced higher hydrolytic activity than Nguni cow faeces and soil controls, thereby confirming that these materials are potential targets for hydrolytic enzyme mining.

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Section: Discussionsupporting

confidence: 93%

Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms

Ndlela

Schmidt

2016

SpringerPlus

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“…This is consistent with the study by De Oliveira et al (2013) , in which Bacteroidetes and Firmicutes are found predominantly in the rumen from steer. In the present study, the reduction of Bacteroidetes and other taxa may be due to the competition of faster growing cellulolytic bacteria belonging to Firmicutes in obtaining nutrients or energy ( Witten and Richardson, 2003 ; Theodorou et al, 2005 ).…”

Section: Discussionmentioning

confidence: 83%

Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal Fermentation and Microbial Community in in vitro Incubation of Agricultural Straws

Liu

et al. 2018

Front. Microbiol.

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Agricultural straws, such as rice straw, wheat straw, and corn straw, are produced abundantly every year but not utilized efficiently in China. An experiment was conducted to determine the effects of recombinant xylanase on ruminal fermentation and microbial community structure in in vitro incubation of these straws. The recombinant xylanase from Lentinula edodes (rLeXyn11A) was produced in Pichia pastoris. The optimal temperature and pH for rLeXyn11A were 40°C and 4.0, respectively. The rLeXyn11A featured resistance to high temperature and showed broad temperature adaptability (>50% of the maximum activity at 20–80°C). Supplemental rLeXyn11A enhanced the hydrolysis of three agricultural straws. After in vitro ruminal incubation, regardless of agricultural straws, the fiber digestibility, acetate concentration, total volatile fatty acids (VFAs) production, and fermentation liquid microbial protein were increased by rLeXyn11A. Supplemental rLeXyn11A increased the ammonia-N concentration for corn straw and rice straw. High throughput sequencing and real-time PCR data showed that the effects of rLeXyn11A on ruminal microbial community depended on the fermentation substrates. With rice straw, rLeXyn11A increased the relative abundance of fibrolytic bacteria including Firmicutes, Desulfovibrio, Ruminococcaceae and its some genus, and Fibrobacter succinogenes. With wheat straw, rLeXyn11A increased the relative abundance of Ruminococcus_1 and its three representative species F. succinogenes, Ruminococcus flavefaciens, Ruminococcus albus. With corn straw, the fibrolytic bacteria Firmicutes, Christensenellaceae_R_7_group, Saccharofermentans, and Desulfovibrio were increased by rLeXyn11A. This study demonstrates that rLeXyn11A could enhance in vitro ruminal digestion and fermentation of agricultural straws, showing the potential of rLeXyn11A for improving the utilization of agricultural straws in ruminants.

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“…Additionally, research conducted on mice has also demonstrated that those fed with a high-fiber diet display a higher F/B ratio compared to those on a low-fiber diet [46]. Therefore, we speculated that the decline in Bacteroidota might arise from competition for nutrients or energy with the faster-growing fiber-degrading bacteria in Firmicutes [47]. Clostridia_UCG-014 negatively correlates with liver function indicators, particularly ALT levels [48].…”

Section: Discussionmentioning

confidence: 96%

Effects of Dietary Alfalfa Meal Supplementation on the Growth Performance, Nutrient Apparent Digestibility, Serum Parameters, and Intestinal Microbiota of Raccoon Dogs (Nyctereutes procyonoides)

Chen,

Li,

Chen

et al. 2024

Animals

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The raccoon dog (Nyctereutes procyonoides) is a typical omnivore possessing wide dietary adaptability and tolerance to rough feeding, which may be attributed to its intestinal microbiota. The study aimed to investigate the effect of dietary alfalfa meal levels on the growth performance, nutrient apparent digestibility, serum parameters, and intestinal microbiota of raccoon dogs. Sixty raccoon dogs were randomly divided into four dietary treatments containing 0% (AM0), 5% (AM5), 10% (AM10), and 15% (AM15) alfalfa meal for a 60-day experiment. The results showed that compared to raccoon dogs fed the AM0 diet, those fed the AM5 and AM10 diets had no significant difference in growth performance, while those fed the AM15 diet experienced a significant decrease. Raccoon dogs fed the AM5 diet had no significant effect on the nutrient apparent digestibility. Dietary supplementation with alfalfa meal significantly decreased serum urea levels and increased the antioxidant capacity of raccoon dogs. The intestinal microbiome analysis showed that the richness and diversity of colonic microbiota significantly increased in the AM15 group. With the increase in dietary alfalfa meal levels, the relative abundance of fiber-degrading bacteria in the colon of raccoon dogs, such as Treponema, Phascolarctobacterium, and Christensenellaceae R-7 group, increased. However, the relative abundance of pathogenic bacteria, including Anaerobiospirillum, decreased. In conclusion, the inclusion of 5% alfalfa meal in the raccoon dogs’ diet had no effect on growth performance, but it exhibited the potential to improve serum antioxidant capacity and intestinal microbiota. This indicates that raccoon dogs have a certain tolerance to the addition of alfalfa meal in their diet.

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Competition of three aggregated microbial species for four substrates in the rumen

Cited by 7 publications

References 27 publications

Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms

Evaluation of wild herbivore faeces from South Africa as a potential source of hydrolytically active microorganisms

Expression of a Recombinant Lentinula edodes Xylanase by Pichia pastoris and Its Effects on Ruminal Fermentation and Microbial Community in in vitro Incubation of Agricultural Straws

Effects of Dietary Alfalfa Meal Supplementation on the Growth Performance, Nutrient Apparent Digestibility, Serum Parameters, and Intestinal Microbiota of Raccoon Dogs (Nyctereutes procyonoides)

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