Competitive inhibition of plasma cholesterol esterification by human high-density lipoprotein-subfraction 2

Barter, Philip J.; Hopkins, Garry J.; Gorjatschko, Ludmila; Jones, Michael E.

doi:10.1016/0005-2760(84)90328-x

Cited by 44 publications

(18 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies by Barter et al 24 -25 suggest that a combined effect may have resulted in the elevated esterification rates we have observed. Investigations in their laboratory have shown that HDL 2 may be a competitive inhibitor of LCAT 24 and that plasma of hypertriglyceridemic subjects may have an increased content of small HDL 3 , which have a greater-than-normal reactivity with LCAT. 25 The consensus of these studies is that the smaller (HDL 3 ) particles are the best substrate for the enzyme and that HDL 2 is a competitive inhibitor of this reaction.…”

Section: Discussionmentioning

confidence: 99%

Cholesterol esterification rates in very low density lipoprotein- and low density lipoprotein-depleted plasma. Relation to high density lipoprotein subspecies, sex, hyperlipidemia, and coronary artery disease.

Dobiášová¹,

Stríbrná²,

Sparks³

et al. 1991

Arterioscler Thromb

View full text Add to dashboard Cite

The fractional rate of cholesterol esterification in very low density lipoprotein-and low density lipoprotein-depleted plasma (FERgoJ was studied in normolipidemic subjects and in individuals with hyperlipidemia and proven coronary artery disease (CAD). The FERnm, was significantly higher than the FER in whole plasma and was significantly higher in normal men than in normal women. In addition, men and women with primary hyperlipidemia had significantly

show abstract

Section: Discussionmentioning

confidence: 99%

Cholesterol esterification rates in very low density lipoprotein- and low density lipoprotein-depleted plasma. Relation to high density lipoprotein subspecies, sex, hyperlipidemia, and coronary artery disease.

Dobiášová¹,

Stríbrná²,

Sparks³

et al. 1991

Arterioscler Thromb

View full text Add to dashboard Cite

show abstract

“…4d, % CE͞CHOL͞ 0.5hr]. As LCAT activity is modulated by the structure and composition of the endogenous lipoprotein substrate (16,25,26), we measured endogenous plasma LCAT activity in the two types of mice against their own endogenous lipoproteins (from EL Ϫ/Ϫ or EL ϩ/ϩ mice) as substrates (Fig. 4e).…”

Section: Absence Of El Affects Expression Of Selected Gene Products Tmentioning

confidence: 99%

Endothelial lipase is a major genetic determinant for high-density lipoprotein concentration, structure, and metabolism

Çilingiroğlu

Otvos

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

217

220

View full text Add to dashboard Cite

High-density lipoprotein (HDL) protects against atherosclerosis. Endothelial lipase (EL) has been postulated to be involved in lipoprotein, and possibly HDL, metabolism, yet the evidence has been scarce and conflicting. We have inactivated EL in mice by gene targeting. EL ؊/؊ mice have elevated plasma and HDL cholesterol, and increased apolipoproteins A-I and E. NMR analysis reveals an abundance of large HDL particles. There is down-regulation of the transcripts for phospholipid transfer protein, but up-regulation of those for hepatic lipase and lipoprotein lipase. Plasma lecithin:cholesterol acyltransferase is unchanged despite an increase in hepatic mRNA; lecithin:cholesterol acyltransferase activity toward endogenous EL ؊/؊ substrate is, however, reduced by 50%. HDL clearance is decreased in EL ؊/؊ mice; both the structure of HDL and the presence of EL are factors that determine the rate of clearance. To determine EL's role in humans, we find a significant association between a single-nucleotide polymorphism 584C͞T in the EL (LIPG) gene and HDL cholesterol in a well characterized population of 372 individuals. We conclude that EL is a major determinant of HDL concentration, structure, and metabolism in mice, and a major determinant of HDL concentration in humans.lipoprotein metabolism ͉ atherosclerosis ͉ gene targeting

show abstract

“…35,36 Furthermore, previous studies have revealed that cholesterol esterification in incubated plasma is positively associated with increasing TG-rich lipoprotein concentrations using both the endogenous, [36][37][38][39] and the exogenous substrate methods, 40 and is negatively associated with increasing HDL concentrations using the endogenous substrate methods. 36,38,39 Moreover, HDL 2 has been proposed as a competitive inhibitor of LCAT, 36,37,41,42 whereas HDL 3 has been reported to be a favorable substrate for LCAT with the use of endogenous substrate method or the reconstituted model using purified LCAT and isolated lipoproteins. [41][42][43] It should be noted that genetically deficient LCAT individuals are different from individuals from the general population.…”

Section: Discussionmentioning

confidence: 99%

Increased Serum Cholesterol Esterification Rates Predict Coronary Heart Disease and Sudden Death in a General Population

Tanaka

Yasuda²,

Ishida³

et al. 2013

ATVB

View full text Add to dashboard Cite

Objective-Lecithin:cholesterol acyltransferase (LCAT) is thought to be important in reverse cholesterol transport. However, its association with coronary heart disease (CHD) and sudden death is controversial. Approach and Results-We prospectively studied 1927 individuals from the general population. Serum concentrations of apolipoprotein A-I, A-II, B, C-II, C-III, E, and LCAT activity measured as a serum cholesterol esterification rate were evaluated. We documented 61 events of CHD and sudden death during 10.9 years of follow-up. After adjustment for age and sex, LCAT activity was significantly associated with the risk of CHD and sudden death (hazard ratio, 3.02; 95% confidence interval, 1.49−6.12; P=0.002). In multivariate analysis adjusted for age, sex, current smoking status, history of diabetes mellitus, body mass index, systolic blood pressure, serum total cholesterol, and serum high-density lipoprotein cholesterol concentrations, the hazard ratio of LCAT activity for the risk of CHD and sudden death remained significant (hazard ratio, 3.07; 95% confidence interval, 1.35−7.01; P=0.008). However, when it was analyzed for men and women separately, this association remained significant only in women. Conclusions-Increased LCAT activity measured as a serum cholesterol esterification rate was a risk for CHD and sudden death in a Japanese general population. Key Words: cohort study ◼ coronary heart disease ◼ lecithin:cholesterol acyltransferase ◼ sex differences ◼ sudden death Increased Serum Cholesterol Esterification Rates Predict Coronary Heart Disease and Sudden Death in a General PopulationShin-ichiro Tanaka Tanaka et al LCAT Activity Predicts CHD and Sudden Death 1099measured by the exogenous substrate method. However, this relationship was not significant after adjustment for lipids. 21 Thus, the number of prospective studies in humans is still limited, and the results are controversial. 12 Here, we report that increased LCAT activity measured as a serum cholesterol esterification rate by the endogenous substrate method is significantly associated with a future risk of CHD and sudden death in a community-based cohort study. Materials and MethodsMaterials and Methods are available in the online-only Supplement. ResultsBaseline characteristics of the study cohort are presented in Table 1. Women had higher concentrations of total cholesterol (TC), HDL-C, and non-HDL-C than men (all tests for trend, P<0.001). Men had higher proportions of current smokers (P<0.001) and patients with a history of diabetes mellitus (P=0.03), a higher serum concentration of triglycerides (TG) (P<0.001), and a higher level of serum thiobarbituric acid reactive substances (TBARS) (P=0.001), than Women.During the 19 866 person-years of follow-up (median followup period, 10.9 years), we documented 19 cases of myocardial infarction (15 in men and 4 in women); 12 cases of angina pectoris, coronary artery bypass, and angioplasty procedures (10 in men and 2 in women); and 30 cases of sudden deaths (13 in men and 17 in women). Thus, the t...

show abstract

Competitive inhibition of plasma cholesterol esterification by human high-density lipoprotein-subfraction 2

Cited by 44 publications

References 17 publications

Cholesterol esterification rates in very low density lipoprotein- and low density lipoprotein-depleted plasma. Relation to high density lipoprotein subspecies, sex, hyperlipidemia, and coronary artery disease.

Cholesterol esterification rates in very low density lipoprotein- and low density lipoprotein-depleted plasma. Relation to high density lipoprotein subspecies, sex, hyperlipidemia, and coronary artery disease.

Endothelial lipase is a major genetic determinant for high-density lipoprotein concentration, structure, and metabolism

Increased Serum Cholesterol Esterification Rates Predict Coronary Heart Disease and Sudden Death in a General Population

Contact Info

Product

Resources

About