Competitive interaction on dual‐species biofilm formation by spoilage bacteria, Shewanella baltica and Pseudomonas fluorescens

Abstract

Aims: This study aims to characterize the biofilm produced by mono-and dual-species of Shewanella baltica and Pseudomonas fluorescens as fish spoilers at the different incubation temperature, and to elucidate the interactive behaviour of dual-species biofilm development. Methods and Results: The mono-and dual-species biofilm formation and adhesion characteristics of S. baltica and P. fluorescens were evaluated by using crystal violet staining, scanning electron microscopy and confocal laser scanning microscopy. Results showed that P. fluorescens had significantly higher biofilm biomass and polysaccharides production than S. baltica, and two isolates reached the maximum biofilm biomass during the early stationary phase. Lower biomass and polysaccharides in dual-species biofilms were observed compared to mono-species of P. fluorescens. Meanwhile, S. baltica and P. fluorescens formed fragile and viscous pellicles with different spatial architectures respectively. In dual-species pellicle few large microcolonies were dominated by P. fluorescens. Compared to mono-species of PF07, adherent cell population and biofilm thickness at the developing phase significantly decreased, and biofilm-forming cycle prolonged in the dual-species biofilms. Biofilm formation and adhesion of mono-and dual-species at 4 or 15°C were significantly higher than at 30°C during the same phase. The culture supernatant extracts of the two spoilage strains greatly inhibited biofilm development to each other. Conclusions: Shewanella baltica and P. fluorescens had different biofilm and pellicle characteristics, and the inhibitory development on dual-species biofilm was associated with the competitive interaction by the two psychrotrophic spoilage bacteria. Significance and Impact of the Study: This work contributes to a better understanding of interactive behaviour of multispecies biofilm communities by psychrotrophic spoilage bacteria at low temperature, which could contribute to further control contamination of spoilage organism during the preservation and processing of aquatic products.

Comparative Proteome Analysis of Shewanella putrefaciens WS13 Mature Biofilm Under Cold Stress

“…Biofilms forming assay was carried out as described previously (Zhu et al, 2019) with slight modifications. The overnight broth cultures of S. putrefaciens WS13 were grown to approximately 8 log CFU mL −1 (OD 600 ≈0.8) and diluted with fresh sterile LB medium (1:100), then added 1 mL of dilution culture to 24-well polystyrene microtiter plates.…”

Synergism With ε-Polylysine Hydrochloride and Cinnamon Essential Oil Against Dual-Species Biofilms of Listeria monocytogenes and Pseudomonas lundensis

“…Similarly, various Pseudomonas spp. readily form biofilms under the chilled conditions, such as P. fragi in chilled meat (Wickramasinghe et al, 2019), P. lundensis in pork meat (Liu et al, 2015), and P. fluorescens in fish (Zhu et al, 2019). Moreover, PL were here found to clearly dominate in the co-culture biofilm communities during the incubation period, rather than planktonic state.…”

Formation of Multispecies Biofilms and Their Resistance to Disinfectants in Food Processing Environments: A Review

“…Chandler et al (11) reported that AHL is indispensable for inter-species competition. Zhu et al (114) found that there are competitive interactions between Pseudomonas fluorescens and Shewanella baltica, and S. baltica may inhibit the production of AHL in dual-species biofilms or consume the AHL secreted by P. fluorescens. de Almeida et al (16) revealed that Salmonella Enteritidis can form stronger biofilms in the presence of AHL from other species.…”