Competitive ion binding to low density lipoproteins: an electron spin resonance study

Iodide and acrylamide were applied simultaneously in a double-quenching experiment to compare acrylamide quenching constants for internal and external fluorophores of high-density lipoproteins (HDL1 and HDL2) from manganese-adequate (MnA) and deficient (MnD) rats, free of the electrostatic effects associated with iodide. In MnA HDL1 compared to MnD HDL1, the acrylamide quenching constant for external fluorophores was different (P < 0.1). In MnA HDL2, there were two populations of fluorophores accessible to acrylamide, whereas in MnD HDL2, all fluorophores were accessible to both quenchers. We concluded that there were structural (local environmental) differences, possibly charge-related, around the external fluorophores, and a slightly larger population of buried fluorophores in the MnD HDL1 compared with MnA HDL1. In MnA HDL2, one-third of the fluorophores were accessible to iodide, and all external and internal fluorophores were accessible to acrylamide, whereas in MnD HDL2, all fluorophores were accessible to both quenchers.

show abstract

A fluorescence double-quenching study of native lipoproteins in an animal model of manganese deficiency

Taylor

Patterson

Klimis-Tavantzis

1997

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

Chemical modification of low-density lipoprotein enhances the number of binding sites for divalent cations

Pifat

Brnjas-Kraljević

Jürgens

et al. 1992

Chemistry and Physics of Lipids

View full text Add to dashboard Cite

competitive binding at the surface of dodecylsulfate/brij 35 mixed micelles

Abuín¹,

Lissi²

1992

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Competitive ion binding to low density lipoproteins: an electron spin resonance study

Cited by 5 publications

References 23 publications

A fluorescence double-quenching study of native lipoproteins in an animal model of manganese deficiency

A fluorescence double-quenching study of native lipoproteins in an animal model of manganese deficiency

Chemical modification of low-density lipoprotein enhances the number of binding sites for divalent cations

competitive binding at the surface of dodecylsulfate/brij 35 mixed micelles

Contact Info

Product

Resources

About