Competitive protein adsorption on biomaterial surface studied with reflectometric interference spectroscopy

Huang, Yan; Lü, Xiaoying; Qian, Weiping; Tang, Zuming; Zhong, Yinping

doi:10.1016/j.actbio.2009.12.035

Cited by 28 publications

(26 citation statements)

References 37 publications

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“…The protein adsorption ratio of FBG/BSA for the TPUs, was in the range from 0.086 to 0.147. All TPU samples preferred to adsorb BSA, which was supposed to be beneficial for biocompatibility, according to findings of other authors [56,57].…”

Section: Protein Adsorption On the Tpu Filmssupporting

confidence: 68%

In Vitro Biocompatibility Evaluation of Novel Urethane–Siloxane Co-Polymers Based on Poly( ϵ -Caprolactone)- block -Poly(Dimethylsiloxane)- block -Poly( ϵ -Caprolactone)

Pergal

Antić

Tovilović³

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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Novel polyurethane co-polymers (TPUs), based on poly(ϵ-caprolactone)-block-poly(dimethylsiloxane)-block-poly(ϵ-caprolactone) (PCL-PDMS-PCL) as soft segment and 4,4'-methylenediphenyl diisocyanate (MDI) and 1,4-butanediol (BD) as hard segment, were synthesized and evaluated for biomedical applications. The content of hard segments (HS) in the polymer chains was varied from 9 to 63 wt%. The influence of the content and length of the HS on the thermal, surface, mechanical properties and biocompatibility was investigated. The structure, composition and HS length were examined using (1)H- and quantitative (13)C-NMR spectroscopy. DSC results implied that the synthesized TPUs were semicrystalline polymers in which both the hard MDI/BD and soft PCL-PDMS-PCL segments participated. It was found that an increase in the average HS length (from 1.2 to 14.4 MDI/BD units) was accompanied by an increase in the crystallinity of the hard segments, storage moduli, hydrophilicity and degree of microphase separation of the co-polymers. Depending on the HS content, a gradual variation in surface properties of co-polymers was revealed by FT-IR, AFM and static water contact angle measurements. The in vitro biocompatibility of co-polymers was evaluated using the endothelial EA.hy926 cell line and protein adsorption on the polyurethane films. All synthesized TPUs adsorbed more albumin than fibrinogen from multicomponent protein mixture, which may indicate biocompatibility. The polyurethane films with high HS content and/or high roughness coefficient exhibit good surface properties and biocompatible behavior, which was confirmed by non-toxic effects to cells and good cell adhesion. Therefore, the non-cytotoxic chemistry of the co-polymers makes them good candidates for further development as biomedical implants.

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Section: Protein Adsorption On the Tpu Filmssupporting

confidence: 68%

In Vitro Biocompatibility Evaluation of Novel Urethane–Siloxane Co-Polymers Based on Poly( ϵ -Caprolactone)- block -Poly(Dimethylsiloxane)- block -Poly( ϵ -Caprolactone)

Pergal

Antić

Tovilović³

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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“…It is possible that changes in surface chemistry affect protein adsorption and/or extracellular matrix deposition, facilitating expression of specific integrins. Several reports have shown preferential protein adsorption in different chemistries, supporting this hypothesis [56,57]. …”

Section: Discussionsupporting

confidence: 55%

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

et al. 2015

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Surface roughness, topography, chemistry, and energy promote osteoblast differentiation and increase osteogenic local factor production in vitro and bone-to-implant contact in vivo, but the mechanisms involved are not well understood. Knockdown of integrin heterodimer alpha2beta1 (α2β1) blocks the osteogenic effects of the surface, suggesting signaling by this integrin homodimer is required. The purpose of the present study was to separate effects of surface chemistry and surface structure on integrin expression by coating smooth or rough titanium (Ti) substrates with graphitic carbon, retaining surface morphology but altering surface chemistry. Ti surfaces (smooth [Ra<0.4μm], rough [Ra≥3.4μm]) were sputter-coated using a magnetron sputtering system with an ultrapure graphite target, producing a graphitic carbon thin film. Human mesenchymal stem cells and MG63 osteoblast-like cells had higher mRNA for integrin subunits α1, α2, αv, and β1 on rough surfaces in comparison to smooth, and integrin αv on graphitic-carbon-coated rough surfaces in comparison to Ti. Osteogenic differentiation was greater on rough surfaces in comparison to smooth, regardless of chemistry. Silencing integrins β1, α1, or α2 decreased osteoblast maturation on rough surfaces independent of surface chemistry. Silencing integrin αv decreased maturation only on graphitic carbon-coated surfaces, not on Ti. These results suggest a major role of the integrin β1 subunit in roughness recognition, and that integrin alpha subunits play a major role in surface chemistry recognition.

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“…Protein adsorption on the surface of biomedical materials could represent a problem since it can simulate deleterious biological processes and lead to thrombosis, embolism, lysis and inflammation. Recent articles suggested that designing the materials that specifically reduce the adsorption of proteins involved in blood clothing process rather than total protein adsorption, might be more efficient way for achieving biocompatibility of the materials . Our in vitro measurements of plasma protein adsorption suggested good blood compatibility of novel polyurethanes.…”

Section: Discussionmentioning

confidence: 65%

Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells

Stefanović

Djonlagić

Tovilović

et al. 2014

J. Biomed. Mater. Res.

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Polyurethane copolymers based on α,ω-dihydroxypropyl poly(dimethylsiloxane) (PDMS) with a range of soft segment contents were prepared by two-stage polymerization, and their microstructures, thermal, thermomechanical, and surface properties, as well as in vitro hemo- and cytocompatibility were evaluated. All utilized characterization methods confirmed the existence of moderately microphase separated structures with the appearance of some microphase mixing between segments as the PDMS (i.e., soft segment) content increased. Copolymers showed higher crystallinity, storage moduli, surface roughness, and surface free energy, but less hydrophobicity with decreasing PDMS content. Biocompatibility of copolymers was evaluated using an endothelial EA.hy926 cell line by direct contact, an extraction method and after pretreatment of copolymers with multicomponent protein mixture, as well as by a competitive protein adsorption assay. Copolymers showed no toxic effect to endothelial cells and all copolymers, except that with the lowest PDMS content, exhibited resistance to endothelial cell adhesion, suggesting their unsuitability for long-term biomedical devices which particularly require re-endothelialization. All copolymers exhibited excellent resistance to fibrinogen adsorption and adsorbed more albumin than fibrinogen in the competitive adsorption assay, suggesting their good hemocompatibility. The noncytotoxic chemistry of these synthesized materials, combined with their nonadherent properties which are inhospitable to cell attachment and growth, underlie the need for further investigations to clarify their potential for use in short-term biomedical devices.

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Competitive protein adsorption on biomaterial surface studied with reflectometric interference spectroscopy

Cited by 28 publications

References 37 publications

In Vitro Biocompatibility Evaluation of Novel Urethane–Siloxane Co-Polymers Based on Poly( ϵ -Caprolactone)- block -Poly(Dimethylsiloxane)- block -Poly( ϵ -Caprolactone)

In Vitro Biocompatibility Evaluation of Novel Urethane–Siloxane Co-Polymers Based on Poly( ϵ -Caprolactone)- block -Poly(Dimethylsiloxane)- block -Poly( ϵ -Caprolactone)

Role of integrin subunits in mesenchymal stem cell differentiation and osteoblast maturation on graphitic carbon-coated microstructured surfaces

Poly(urethane-dimethylsiloxane) copolymers displaying a range of soft segment contents, noncytotoxic chemistry, and nonadherent properties toward endothelial cells

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