Competitive reactions in solutions of DNA and water‐soluble interpolyelectrolyte complexes

Izumrudov, Vladimir A.; Kargov, S. I.; Zhiryakova, Marina V.; Зезин, А. Б.; Kabanov, V.A.

doi:10.1002/bip.360350511

Cited by 44 publications

(47 citation statements)

References 16 publications

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“…Release of EB from the (DNA N EB) complex causes a decrease in the quantum yield and has been used to study the association of low molecular weight polyamines (analogs of spermine) with calf thymus DNA in vitro 20) . The fluorescence spectroscopic assay proved to be applicable for analyzing of the complex formation between DNA and different polycations, in particular linear synthetic polyamines, basic polypeptides, and hystones 18) . The developed approach is appropriate for monitoring the destruction of DNA-containing PEC in water-salt solutions as well, because the EB fluorescence caused by intercalation of the dye into free sites of the DNA double helix was observed in the range of salt concentration corresponding to the dissociation of the complex (DNA N EB-polycation) into the components, i. e., the complex (DNA N EB) and the polycation 18) .…”

Section: Resultsmentioning

confidence: 99%

“…The fluorescence spectroscopic assay proved to be applicable for analyzing of the complex formation between DNA and different polycations, in particular linear synthetic polyamines, basic polypeptides, and hystones 18) . The developed approach is appropriate for monitoring the destruction of DNA-containing PEC in water-salt solutions as well, because the EB fluorescence caused by intercalation of the dye into free sites of the DNA double helix was observed in the range of salt concentration corresponding to the dissociation of the complex (DNA N EB-polycation) into the components, i. e., the complex (DNA N EB) and the polycation 18) . Fluorimetric titration curves of solutions of the (DNA N EB) complex (curve 1) and PEC (DNA N EB-PEVP) (curve 2) by salt are represented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It suggests that practically all EB molecules are squeezed out by the added polycation. Curve 2 has a sigmoid shape with the right-handed branch corresponding to the dissociation of PEC 18) . The coincidence of both curves at C NaCl = 0.35 mol/L indicates a complete liberation of the polycation from PEC.…”

Section: Resultsmentioning

confidence: 99%

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Stability of DNA-containing interpolyelectrolyte complexes in water-salt solutions

Izumrudov¹,

Zhiryakova

1999

Macromol. Chem. Phys.

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SUMMARY: Destruction of interpolyelectrolyte complexes (PEC) formed by DNA and different poly cations was achieved by addition of low molecular weight electrolytes. Monitoring of PEC dissociation was carried out by fluorescence quenching using the ability of the cationic dye ethidium bromide to intercalate into free sites of the DNA double helix accompanied by fluorescence. Degree of polymerization and charge density of the polycations as well as their N-alkyl substituentes (alkyl = methyl, ethyl, and propyl) were shown to be factors influencing the stability of PEC. The ability of added cations and anions to dissociate PEC decreases in the order Cawhich coincides with a decrease of affinity of the same counterions to DNA and to the polycation. The data obtained indicate that the change of the stability of DNA-containing PEC shows the same regularities as revealed for the stability of interpolyelectrolyte complexes formed by oppositely charged flexible synthetic polyelectrolytes in water-salt solutions.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Stability of DNA-containing interpolyelectrolyte complexes in water-salt solutions

Izumrudov¹,

Zhiryakova

1999

Macromol. Chem. Phys.

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“…The literature on this subject is extensive [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] and there are also several excellent reviews describing the phenomenology of solutions of oppositely charged polyelectrolytes. [1][2][3] There are also many simulation and theoretical investigations related to adsorption of polyelectrolytes to oppositely charged solid surfaces and inter-chain interactions.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the substitution reaction (second step) in Fig. 1, extensive kinetic studies were conducted some time ago by the Kabanov and Dautzenberg schools, [5][6][7][8][9][10][12][13][14] with many unexpected findings. For example, smaller polycations would complex first with a longer polyanion before these get displaced ultimately by longer polycations, demonstrating an interplay between kinetics and thermodynamics.…”

Section: Introductionmentioning

confidence: 99%

Modeling competitive substitution in a polyelectrolyte complex

Peng

Muthukumar

2015

The Journal of Chemical Physics

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We have simulated the invasion of a polyelectrolyte complex made of a polycation chain and a polyanion chain, by another longer polyanion chain, using the coarse-grained united atom model for the chains and the Langevin dynamics methodology. Our simulations reveal many intricate details of the substitution reaction in terms of conformational changes of the chains and competition between the invading chain and the chain being displaced for the common complementary chain. We show that the invading chain is required to be sufficiently longer than the chain being displaced for effecting the substitution. Yet, having the invading chain to be longer than a certain threshold value does not reduce the substitution time much further. While most of the simulations were carried out in salt-free conditions, we show that presence of salt facilitates the substitution reaction and reduces the substitution time. Analysis of our data shows that the dominant driving force for the substitution process involving polyelectrolytes lies in the release of counterions during the substitution. C 2015 AIP Publishing LLC. [http://dx

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Temperature controllable interpolyelectrolyte substitution reactions

Izumrudov

Ortega-Ortíz

Зезин

et al. 1998

Macromol. Chem. Phys.

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SUMMARY The competitive binding of fluorescence-tagged poly(methacry1ate) polyanions and poly(phosphate) polyanions with different poly(N-alkyl-4-vinylpyridinium) polycation quenchers (alkyl = methyl, ethyl and propyl) was studied in the region 278+333 K by fluorescence quenching technique. The equilibrium of this interpolyelectrolyte substitution reaction proved to be temperature sensitive except in the case when the poly(N-methyl-4-vinylpyridinium) polycation was used. Thermodynamic parameters of the reaction were determined. The possibility of effective temperature control of the interpolyelectrolyte reaction might be important for understanding, prediction and utilization of transformation processes of complex particles formed by electrostatically complementary (biological) macromolecules.

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Competitive reactions in solutions of DNA and water‐soluble interpolyelectrolyte complexes

Cited by 44 publications

References 16 publications

Stability of DNA-containing interpolyelectrolyte complexes in water-salt solutions

Stability of DNA-containing interpolyelectrolyte complexes in water-salt solutions

Modeling competitive substitution in a polyelectrolyte complex

Temperature controllable interpolyelectrolyte substitution reactions

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