Competitive reduction of poultry-borne enteric bacterial pathogens in chicken gut with bioactive Lactobacillus casei

Tabashsum, Zajeba; Peng, Mengfei; Alvarado-Martinez, Zabdiel; Aditya, Arpita; Bhatti, Jacob; Romo, Paulina Bravo; Young, Alana; Biswas, Debabrata

doi:10.1038/s41598-020-73316-5

Cited by 29 publications

(25 citation statements)

References 52 publications

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“…In our previous study, probiotic supplementation increased the abundance level of Parasutterella in the gut of Salmonella Typhimurium challenged laying chickens [6]. Parasutterella has shown to increase in abundance in the gut of pigs that received a prebiotic (waxy corn starch) supplemented diet [48], and in the gut of SPF chickens that received Lactobacillus casei supplemented diet [49]. In laying chickens gut, Paraprevotella is involved in polysaccharide degradation, and propionate and butyrate production through the expression of xylose isomerase, cobalamin-binding methylmalonyl-CoA mutase and/or methylmalonyl-CoA epimerase and acetyl-CoA acetyltransferase, respectively [50].…”

Section: Discussionmentioning

confidence: 91%

Functional enrichment of gut microbiome by early supplementation of Bacillus based probiotic in cage free hens: a field study

Khan

Chousalkar

2021

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Background The chicken gut microbiota passes through different stages of maturation; therefore, strengthening it with well characterised probiotics increases its resilience required for optimum gut health and wellbeing. However, there is limited information on the interaction of Bacillus based probiotics with gut microbial community members in cage free laying chickens both in rearing and production phases of life. In the current study, we investigated the changes in the gut microbiome of free range hens in the field after Bacillus based probiotic supplementation. Results Overall, at phylum level, probiotic supplementation increased the populations of Bacteroidetes and Proteobacteria mainly at the expense of Firmicutes. The population of Bacteroidetes significantly increased during the production as compared to the rearing phase, and its higher population in the probiotic-supplemented chickens reflects the positive role of Bacillus based probiotic in gut health. Core differences in the beta diversity suggest that probiotic supplementation decreased microbial compositionality. The non-significant difference in alpha diversity between the probiotic and control chickens showed that the composition of community structure did not change. No Salmonella spp. were isolated from the probiotic supplemented birds. Egg internal quality was significantly higher, while egg production and body weight did not differ. Functional prediction data showed that probiotic supplementation enriched metabolic pathways, such as vitamin B6 metabolism, phenylpropanoid biosynthesis, monobactam biosynthesis, RNA degradation, retinol metabolism, pantothenate and CoA biosynthesis, phosphonate and phosphinate metabolism, AMPK signaling pathway, cationic antimicrobial peptide (CAMP) resistance and tyrosine metabolism. Conclusions Overall, age was the main factor affecting the composition and diversity of gut microbiota, where probiotic supplementation improved the abundance of many useful candidates in the gut microbial communities. The generated baseline data in the current study highlights the importance of the continuous use of Bacillus based probiotic for optimum gut health and production.

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Section: Discussionmentioning

confidence: 91%

Functional enrichment of gut microbiome by early supplementation of Bacillus based probiotic in cage free hens: a field study

Khan

Chousalkar

2021

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“…Despite the amount of research that has been performed on this subject, it is likely that the source and transmission routes of C. jejuni invasion in broiler farms have farm-to-farm variations (8). Comparatively, many attempts have been made to reduce this pathogen in the broilers during farm rearing using phage therapy (9), feed and/or water supplementation with short-or mediumchain fatty acids (FAs) (10)(11)(12), essential oils (13), organic acids (14), and probiotics (15), as practical control measures. These possible effectors are thought to have either direct or indirect bactericidal effects; however, further study would be required to clarify molecular basis of these approaches in terms of reducing Campylobacter in the field.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Grow-Out Affects Campylobacter jejuni Colonization Fitness in Coincidence With Altered Microbiota and Lipid Composition in the Cecum of Laying Hens

et al. 2021

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Campylobacter jejuni is one of the leading causes of gastrointestinal illness worldwide and is mainly transmitted from chicken through the food chain. Previous studies have provided increasing evidence that this pathogen can colonize and replicate in broiler chicken during its breeding; however, its temporal kinetics in laying hen are poorly understood. Considering the possible interaction between C. jejuni and gut microbiota, the current study was conducted to address the temporal dynamics of C. jejuni in the cecum of laying hen over 40 weeks, with possible alteration of the gut microbiota and fatty acid (FA) components. Following oral infection with C. jejuni 81-176, inocula were stably recovered from ceca for up to 8 weeks post-infection (p.i.). From 16 weeks p.i., most birds became negative for C. jejuni and remained negative up to 40 weeks p.i. 16S rRNA gene sequencing analyses revealed that most of the altered relative rRNA gene abundances occurred in the order Clostridiales, in which increased relative rRNA gene abundances were observed at >16 weeks p.i. in the families Clostridiaceae, Ruminococcaceae, Lachnospiraceae, and Peptococcaceae. Lipidome analyses revealed increased levels of sterols associated with bile acid metabolisms in the cecum at 16 and/or 24 weeks p.i. compared with those detected at 8 weeks p.i., suggesting that altered microbiota and bile acid metabolism might underlie the decreased colonization fitness of C. jejuni in the gut of laying hens.

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“…Phytochemicals can disrupt the cellular membrane of pathogenic microorganisms 55,56 . Further, phytochemicals can promote the hydrophobicity of the microbial species that could affect the surface properties of microbial cells thereby influencing the virulence properties of the microbes 63,64 . The OLP‐supplemented birds presented a higher ( P < 0.05) Lactobacillus spp.…”

Section: Resultsmentioning

confidence: 99%

Onion leaf and synthetic additives in broiler diet: impact on splenic cytokines, serum immunoglobulins, cecal bacterial population, and muscle antioxidant status

et al. 2021

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BACKGROUND The disadvantages associated with the use of synthetic additives in animal production could threaten human and animal health, and the safety of animal‐derived foods. This study assessed the growth performance, blood chemistry, immune indices, selected caeca bacterial population, muscle antioxidant enzyme activities, and meat quality in broiler chickens fed diet supplemented with antibiotic (70% oxytetracycline +30% neomycin), tert‐butylhydroxytoluene or onion leaf powder (OLP). One day old Ross 308 chicks (n = 240) were assigned randomly to either D‐1, control diet (CD) without additives; D‐2, CD + 0.3 g kg−1 antibiotic +0.15 g kg−1 tert‐butylhydroxytoluene; D‐3, CD + 2.5 g kg−1 OLP; or D‐4, CD + 5 g kg−1 OLP for 42 days. RESULTS The D‐2 and D‐4 diets improved (P < 0.05) bodyweight gain and feed efficiency in broilers. Platelet and cecal Lactobacillus spp. counts were higher (P < 0.05) whereas muscle cholesterol was lower (P < 0.05) in the OLP‐supplemented birds. Supplemented birds had higher (P < 0.01) splenic interleukin‐10 and lower (P < 0.01) splenic tumor necrosis factor‐α, immunoglobulin A, cecal E. coli and C. perfringens counts compared with the D‐1 birds. The D‐4 birds had the least (P < 0.05) splenic interleukin‐1β. Dietary supplements increased (P < 0.05) catalase, glutathione peroxidase, and total antioxidant capacity, and lowered (P < 0.05) drip loss, malondialdehyde and carbonyl content in breast meat. CONCLUSION Dietary supplementation of 5 g kg−1 OLP exerted antimicrobial, immunomodulatory, and antioxidant effects that were comparable to those of antibiotics and tert‐butylhydroxytoluene in broiler chickens. © 2021 Society of Chemical Industry.

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Competitive reduction of poultry-borne enteric bacterial pathogens in chicken gut with bioactive Lactobacillus casei

Cited by 29 publications

References 52 publications

Functional enrichment of gut microbiome by early supplementation of Bacillus based probiotic in cage free hens: a field study

Functional enrichment of gut microbiome by early supplementation of Bacillus based probiotic in cage free hens: a field study

Long-Term Grow-Out Affects Campylobacter jejuni Colonization Fitness in Coincidence With Altered Microbiota and Lipid Composition in the Cecum of Laying Hens

Onion leaf and synthetic additives in broiler diet: impact on splenic cytokines, serum immunoglobulins, cecal bacterial population, and muscle antioxidant status

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