Competitiveness and symbiotic effectiveness of a R. gallicum strain isolated from root nodules of Phaseolus vulgaris

Mrabet, Moncef; Mhamdi, Ridha; Tajini, Fatma; Tiwari, Ravi; Trabelsi, Mustapha; Aouani, Mohamed Elarbi

doi:10.1016/j.eja.2004.02.006

Cited by 23 publications

(6 citation statements)

References 32 publications

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“…This result confi rms that indigenous rhizobial strains are bett er adaptable to adverse environmental conditions. Benefi cial eff ect of inoculation with indigenous common bean rhizobia was also determined in diff erent studies performed in Brazil (40,41), Tunisia (42) and Spain (43).…”

Section: Resultsmentioning

confidence: 99%

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Pohajda¹,

Babić

Rajnović

et al. 2016

Food Technol. Biotechnol.

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Natural process of symbiotic nitrogen fi xation has a unique role in a sustainable legume production. Nodule bacteria, collectively named rhizobia, in symbiotic associations with legumes enable considerable entries of biologically fi xed nitrogen into ecosystems. In order to maxi- SummaryNodule bacteria (rhizobia) in symbiotic associations with legumes enable considerable entries of biologically fi xed nitrogen into soil. Eff orts are therefore made to intensify the natural process of symbiotic nitrogen fi xation by legume inoculation. Studies of fi eld populations of rhizobia open up the possibility to preserve and probably exploit some indigenous strains with hidden symbiotic or ecological potentials. The main aim of the present study is to determine genetic diversity of common bean rhizobia isolated from diff erent fi eld sites in central Croatia and to evaluate their symbiotic effi ciency and compatibility with host plants. The isolation procedure revealed that most soil samples contained no indigenous common bean rhizobia. The results indicate that the cropping history had a signifi cant impact on the presence of indigenous strains. Although all isolates were found to belong to species Rhizobium leguminosarum, signifi cant genetic diversity at the strain level was determined. Application of both random amplifi cation of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC--PCR) methods resulted in similar grouping of strains. Symbiotic effi ciency of indigenous rhizobia as well as their compatibility with two commonly grown bean varieties were tested in fi eld experiments. Application of indigenous rhizobial strains as inoculants resulted in signifi cantly diff erent values of nodulation, seed yield as well as plant nitrogen and seed protein contents. The most abundant nodulation and the highest plant nitrogen and protein contents were determined in plants inoculated with R. leguminosarum strains S 17/2 and S 21/6 . Although, in general, the inoculation had a positive impact on seed yield, diff erences depending on the applied strain were not determined. The overall results show the high degree of symbiotic effi ciency of the specifi c indigenous strain S 21/6 . These results indicate diff erent symbiotic potential of indigenous strains and confi rmed the importance of rhizobial strain selection. These are the fi rst studies of indigenous common bean rhizobia in Croatia that provide the basis for further characterization and selection of highly effi cient indigenous strains and their potential use in agricultural practice and future research.

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Section: Resultsmentioning

confidence: 99%

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Pohajda¹,

Babić

Rajnović

et al. 2016

Food Technol. Biotechnol.

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“…The best option for developing stress tolerant crops with minimized production costs and environmental hazards can be the use of PGP microbes as stress relievers. Among them, indigenous and native microbes were more effective and competitive as they are well adapted to the local environments (Mrabet et al 2005 ). Rhizobia when used as microbial inoculants have shown many direct and indirect PGP properties including traits for stress are represented in Tables 1 and 2 .…”

Section: Abiotic Stress Resistance Of Rhizobiamentioning

confidence: 99%

Plant growth promoting rhizobia: challenges and opportunities

et al. 2014

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Modern agriculture faces challenges, such as loss of soil fertility, fluctuating climatic factors and increasing pathogen and pest attacks. Sustainability and environmental safety of agricultural production relies on eco-friendly approaches like biofertilizers, biopesticides and crop residue return. The multiplicity of beneficial effects of microbial inoculants, particularly plant growth promoters (PGP), emphasizes the need for further strengthening the research and their use in modern agriculture. PGP inhabit the rhizosphere for nutrients from plant root exudates. By reaction, they help in (1) increased plant growth through soil nutrient enrichment by nitrogen fixation, phosphate solubilization, siderophore production and phytohormones production (2) increased plant protection by influencing cellulase, protease, lipase and β-1,3 glucanase productions and enhance plant defense by triggering induced systemic resistance through lipopolysaccharides, flagella, homoserine lactones, acetoin and butanediol against pests and pathogens. In addition, the PGP microbes contain useful variation for tolerating abiotic stresses like extremes of temperature, pH, salinity and drought; heavy metal and pesticide pollution. Seeking such tolerant PGP microbes is expected to offer enhanced plant growth and yield even under a combination of stresses. This review summarizes the PGP related research and its benefits, and highlights the benefits of PGP rhizobia belonging to the family Rhizobiaceae, Phyllobacteriaceae and Bradyrhizobiaceae.

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“…Nevertheless, it has been proven that these techniques suffer from inaccuracy caused by instability or cross-reaction with indigenous rhizobia. Mrabet et al (2005) used gus-labeled transconjugants to assess the competitiveness of the strain 8a3 under laboratory conditions. However, we did not use this gene labeling approach under field conditions because of environmental considerations.…”

Section: Competitiveness Monitoringmentioning

confidence: 99%

“…The strain 8a3 of R. gallicum isolated from root nodules of common bean cultivated in Cap Bon was selected for its high symbiotic effectiveness (Mhamdi et al, 2002). The competitiveness of strain 8a3 on soil cores, monitored by using the reporter gene gusA, showed that this strain was highly competitive when inoculated into the cultivars Coco and Royalnel (Mrabet et al, 2005). The efficiency of utilization of this strain as an inoculant needs further confirmation under field conditions.…”

Section: Introductionmentioning

confidence: 99%

Rhizobium gallicum as an efficient symbiont for bean cultivation

Mnasri

Tajini

Trabelsi

et al. 2007

Agron. Sustain. Dev.

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Rhizobia are soil bacteria that fix atmospheric nitrogen in symbiosis with legumes in specialized organs called nodules. The legumes thus acquire the autonomy to grow in nitrogen-deficient soils. When nitrogen fixation by indigenous rhizobia is limited, field inoculation with efficient and competitive strains is an economically feasible way to increase production. When the inoculant is made from native strains of rhizobia the success of inoculation should be increased, since local strains are better adapted than commercial inoculants. Here, a Rhizobium gallicum strain, 8a3, previously selected for its competitiveness and symbiotic effectiveness with common bean under laboratory conditions, was tested in field trials in Tunisia. The experiments were conducted in six fields using three common bean cultivars. The majority of the fields showed a low density of the native rhizobia and inefficient nodulation by Sinorhizobium meliloti, a known symbiont of Medicago. Our results show that inoculation with R. gallicum strain 8a3 induced an increase in nodule numbers accompanied by a more than twofold increase in shoot dry yield. Monitoring of the nodulation occupancy through the fingerprinting of the repetitive extragenic palindromic sequences (REP-PCR) showed that strain 8a3 was competitive even in the soil showing a high population density of indigenous R. gallicum, and occupied more than 40% of the nodules. Moreover, in vitro antibiosis assays indicated that strain 8a3 produces antimicrobial activity on agar medium against indigenous common bean rhizobia, including the inefficient strains of S. meliloti. These results point out the benefits that could be achieved by selecting efficient and competitive strains among natural populations of rhizobia. competitiveness / N2 fixation / Phaseolus vulgaris / Rhizobium gallicum

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Competitiveness and symbiotic effectiveness of a R. gallicum strain isolated from root nodules of Phaseolus vulgaris

Cited by 23 publications

References 32 publications

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Genetic Diversity and Symbiotic Efficiency of Indigenous Common Bean Rhizobia in Croatia

Plant growth promoting rhizobia: challenges and opportunities

Rhizobium gallicum as an efficient symbiont for bean cultivation

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