Compilation of a MALDI-TOF mass spectral database for the rapid screening and characterisation of bacteria implicated in human infectious diseases

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

“…The correct identification of the 17 isolates was dependent on the presence of the reference strains in the MALDI-bioTyper 3.0 database because the species of the reference strain will give the closest match for the identification of the tested strain. The use of MALDI-TOF MS is reported as suitable for the identification of anaerobic bacteria (Veloo et al, 2011 MALDI-TOF MS fingerprinting, a higher discrimination potential has been described, allowing the differentiation and correct identification of much close bacterial species and even strains of the same species (Keys et al, 2004;Donohue et al, 2006;Vargha et al, 2006).…”

Species Identification of Food Spoilage and Pathogenic Bacteria by MALDI-TOF Mass Fingerprinting

“…However, the correct identification of the corresponding species is of great importance for food safety and quality, since the pathogenic and spoilage character can vary significantly. With MALDI-TOF MS fingerprinting a higher discrimination potential has been described, allowing the differentiation and correct identification of much close bacterial species and even strains of the same species (Keys et al, 2004;Donohue et al, 2006;Vargha et al, 2006).…”

“…These works (Bernardo et al, 2002;Bright et al, 2002;Keys et al, 2004;Mandrell et al, 2005;Donohue et al, 2006;Carbonnelle et al, 2007;Barbuddhe et al, 2008;Grosse-Herrenthey et al, 2008;Hazen et al, 2009;Alispahic et al, 2010;Ayyadurai et al, 2010;Dubois et al, 2010;Stephan et al, 2010;Stephan et al, 2011). Furthermore, some studies were aimed at the detection of foodborne pathogens and food spoilage bacteria, including genera such as Escherichia, Yersinia, Proteus, Morganella, Salmonella, Staphylococcus, Micrococcus, Lactococcus, Pseudomonas, Leuconostoc and Listeria (Mazzeo et al, 2006).…”

“…For bacterial identification by MALDI-TOF MS fingerprinting, the spectral profile of the strain of interest is compared to a spectral library of reference strains. Several private databases have been created, including spectral profiles of more than 500 bacterial strains, such as The Spectral Archive And Microbial Identification System (Saramis TM ; AnagnosTec GmbH, Potsdam, Germany) (Erhard et al, 2008) and the Microbelynx bacterial identification system (Waters Corporation, Manchester, UK) (Keys et al, 2004;Dare, 2006). The MALDI Biotyper 2.0 (Bruker Daltonics) search against an ample database of more than 1800 bacterial species and new spectral profiles are being added on a daily basis.…”

Which species concept for pathogenic bacteria?An E-Debate

“…After an ecotype has been discovered and characterized, phenotypic diagnostics can be developed to help the clinician rapidly identify a strain to its ecotype (Keys et al, 2004). Beyond the diagnostic benefits, phenotypic analysis is also a valuable foray into the natural history and etiology of pathogenic ecotypes, and as Haroun Shah points out, our attempts to find more discriminative diagnostics can help us to understand a taxon's pathogenic potential.…”