Complement activation and pulmonary leukotasis during nylon fiber filtration leukapheresis

Hammerschmidt, D. E.; Craddock, Philip R.; McCullough, F; Kronenberg, RS; Dalmasso, A. P.; Jacob, HS

doi:10.1182/blood.v51.4.721.bloodjournal514721

Cited by 12 publications

(12 citation statements)

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“…Changes in the complement system have been described in continuous-flow filtration leukapheresis and are purported to explain adverse effects and changes in donor neutrophil counts (Hammerschmidt et a/. 1978, Nusbacher et al 1978, Rubins et a/.…”

Section: Results Of Complement Studiesmentioning

confidence: 99%

Clinical and laboratory effects on donors of intermittent-flow centrifugation platelet-leukapheresis performed with hydroxyethyl starch and citrate

Strauss

Koepke

Maguire

et al. 1980

Clinical &Amp; Laboratory Haematology

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Section: Results Of Complement Studiesmentioning

confidence: 99%

Clinical and laboratory effects on donors of intermittent-flow centrifugation platelet-leukapheresis performed with hydroxyethyl starch and citrate

Strauss

Koepke

Maguire

et al. 1980

Clinical &Amp; Laboratory Haematology

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“…In rats, the lung seems to be the main target organ in the injury of systemic complement activation [2 11. This is probably also true for humans, since intravascular complement activation by certain hemodialysis or apheresis filters leads to the same pulmonary sequestration of neutrophils and impairment of the respiratory function [23]. Therefore, it is not clear why flushing should be the most frequent adverse reaction after IVIG administration.…”

Section: Clinical Relevance Of the Hypotensive Reactionmentioning

confidence: 99%

An Animal Model for the Detection of Hypotensive Side Effects of Immunoglobulin Preparations

et al. 1987

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Intravenous administration of certain immunoglobulin preparations may cause severe adverse reactions, especially in immunodeficient patients. These reactions are generally assumed to be related to the anticomplementary activity of the preparations, caused by IgG aggregates. Because the exact mechanism of the adverse reaction is unknown, we investigated the reactions induced in anesthetized rats on rapid intravenous administration of different human immunoglobulin preparations. The most conspicuous observation was a severe, long-lasting hypotension, induced by standard immunoglobulin preparations (for intramuscular use), which appeared to be independent from the concomitant complement and neutrophil activation. The long-lasting hypotension was not related to the presence of prekallikrein activator, which induced a transient hypotensive reaction only after sensitizing the rats to bradykinin. The reactions appeared to be associated with IgG aggregates. It was found that certain aggregates induced mainly complement activation, whereas others had mainly a hypotensive effect or no effect at all. It was concluded that the rat model provides a sensitive and reproducible test system for vasoactive properties of immunoglobulin preparations for intravenous administration that cannot be predicted from in vitro measurements, such as anticomplementary activity, aggregate content or prekallikrein activator activity. It is suggested that the test may also be used for other plasma products for intravenous administration.

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“…In addition, this study employed leukapheresis of four TBVs, considerably more than is routinely used in clinical practice for peripheral blood stem cell collection. Filtration leukapheresis has previously been reported to induce pulmonary sequestration of neutrophils with accompanying systemic neutropenia and a reduced diffusing capacity for carbon monoxide [39].…”

Section: Discussionmentioning

confidence: 99%

Pulmonary and systemic effects of mononuclear leukapheresis

et al. 2012

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Background and Objectives There is increasing evidence that monocytes play a key role in the pathogenesis of acute lung inflammation. Mononuclear cell (MNC) leukapheresis can be used to remove large numbers of monocytes from circulating blood; however, the detailed characteristics of monocyte subpopulations removed by MNC leukapheresis, and the biological effects on the lung, remain incompletely defined. Material and Methods Six healthy male volunteers underwent MNC leukapheresis of four total blood volumes. Blood was collected at 0, 2, 4, 6, 8 and 24 h; bronchoscopy with bronchoalveolar lavage (BAL) was performed at 8–9 h. Multiparameter flow cytometry was used to identify subpopulations of monocytes in blood and monocyte‐like cells in BAL fluid. Results A median of 5·57 × 109 monocytes were retrieved. Blood monocyte counts indicated that the circulating blood monocyte pool was actively replenished during leukapheresis and subsequently contained a greater proportion of classical (CD14++ CD16−) monocytes. A particular subpopulation of monocyte‐like cells, reminiscent of classical monocytes, was also prominent in BAL fluid after leukapheresis. Conclusion Mononuclear cell leukapheresis was safe. The greater proportion of classical monocytes present in blood after MNC leukapheresis may be clinically significant. MNC leukapheresis also appears to affect the proportion of monocyte‐like cells in the lung; however, we found no evidence that leukapheresis has a clinically important pro‐inflammatory effect in the human lung.

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Complement activation and pulmonary leukotasis during nylon fiber filtration leukapheresis

Cited by 12 publications

References 0 publications

Clinical and laboratory effects on donors of intermittent-flow centrifugation platelet-leukapheresis performed with hydroxyethyl starch and citrate

Clinical and laboratory effects on donors of intermittent-flow centrifugation platelet-leukapheresis performed with hydroxyethyl starch and citrate

An Animal Model for the Detection of Hypotensive Side Effects of Immunoglobulin Preparations

Pulmonary and systemic effects of mononuclear leukapheresis

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