Complement (C3) levels and activation in rabbits experimentally infected with<i>Trypanosoma evansi</i>

Uche, U.E.; Jones, T.W.

doi:10.1080/00034983.1992.11812696

Cited by 8 publications

(9 citation statements)

References 11 publications

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“…Similarly, the polypeptides of 88, 74, 66, 46, 32, and 17 kDa recognized in our study probably correspond to 86, 73, 68 46, 32, and 17 kDa components labeled by antibodies of horses experimentally infected with a Venezuelan isolate of T. evansi (UZCANGA et al, 2002). The increasing numbers of polypeptides recognized by experimental infected bovines, donkeys, dogs, and coatis with the increasing duration of infection in the present study is in agreement with earlier observations made in experimentally infected rabbits (UCHE; JONES; BOID, 1993). The greater number of polypeptides detected in chronic phase of infection is probably due to the release of internal antigens after parasite destruction by variable surface glycoprotein (VSG) specific antibodies.…”

Section: Discussionsupporting

confidence: 92%

“…In the present study, five major polypeptides with apparent molecular weights 88, 74, 66, 32, and 27 kDa recognized by antibodies of experimental animals, appear to correspond to the 85, 75.5, 67, 32.4, and 28 kDa antigens of T. evansi revealed by sera of rabbits experimentally infected with an Indonesian stock of the parasite (UCHE; JONES; BOID, 1993). Similarly, the polypeptides of 88, 74, 66, 46, 32, and 17 kDa recognized in our study probably correspond to 86, 73, 68 46, 32, and 17 kDa components labeled by antibodies of horses experimentally infected with a Venezuelan isolate of T. evansi (UZCANGA et al, 2002).…”

Section: Discussionsupporting

confidence: 48%

“…Few studies on antigenic characterization of T. evansi have been undertaken (UCHE; JONES; BOID, 1993;JONES, 1994;QUEIROZ et al, 2001) and little is known so far regarding the polypeptide recognition patterns by antibodies from different host species.…”

Section: Introductionmentioning

confidence: 99%

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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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The present research investigated the presence of T. evansi antibodies in animals from the subregion of Nhecolandia, in the Pantanal Sul-mato-grossense, by means of an enzyme linked immunosorbent assay (ELISA) and indirect immunofluorescence antibody test (IFAT), and the pattern of polypeptide recognition by sera from experimentally and naturally infected hosts using Western blotting. Serum samples were obtained from bovines (n = 102), horses (n = 98), and dogs (n = 55), and from 32 free-ranging coatis (Nasua nasua). None of the bovines were found positive, while sera from 16 dogs (29%) and 23 horses (23.4%) were positive by ELISA. Sera from 8 coatis (25%) were found positive using IFAT. Western blotting revealed major polypeptides of T. evansi with molecular weight ranging from 74 to 38 kDa. The polypeptides of 66, 48-46, and 38 kDa were identified by sera from experimentally infected bovines, donkeys, dogs, and coatis. The 48-46 and 38 kDa bands were mainly recognized in chronic phase of infection. The antigen with apparent molecular weight of 66 kDa, revealed by antibodies from all experimental animals, was also recognized in sera of horses and dogs from the Pantanal. The 48-46 kDa polypeptide was identified by antibodies from all naturally infected animals and must be further evaluated for use in specific diagnosis of T. evansi infection.Keywords: Trypanosoma evansi, ELISA-test, IFAT, Pantanal mato-grossense, antigenic characterization. ResumoO trabalho de pesquisa investigou a presença de anticorpos anti-T. evansi em animais da sub-região da Nhecolândia, no Pantanal sul-mato-grossense, pelo ensaio imunoenzimático (ELISA) e a reação de imunofluorescência indireta (RIFI). O reconhecimento de polipeptídeos de T. evansi foi realizado pela técnica de "Western blotting", utilizando soros de animais experimentalmente e naturalmente infectados. As amostras de soro foram obtidas de bovinos (n = 102), cavalos (n = 98) e cães (n = 55) e de 32 quatis de vida livre (Nasua nasua) do pantanal mato-grossense. Todos os soros dos bovinos foram negativos, enquanto soros de 16 cães (29%) e 23 cavalos (23,4%) foram positivos pelo ELISA. Soros de oito quatis (25%) foram positivos pela RIFI. Pelo "Western-blotting" foi possível revelar polipeptídeos de T. evansi, com peso molecular variando de 74 a 38 kDa. Os polipeptídeos de 66, 48-46 e 38 kDa foram identificados por soros de bovinos, cavalos, cães e quatis experimentalmente infectados. As bandas de 48-46 e 38 kDa foram reconhecidas principalmente na fase crônica da infecção. O antígeno com peso molecular aparente de 66 kDa, revelado por anticorpos de todos os animais experimentais, também foi reconhecido por soros de cavalos e cães do Pantanal. O polipeptídeo de 48-46 kDa foi identificado por anticorpos de todos os animais naturalmente infectados, devendo ser avaliado para o diagnóstico específico da infecção pelo T. evansi.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 48%

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Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Aquino¹,

Machado²,

Lemos³

et al. 2010

Rev. Bras. Parasitol. Vet. (Online)

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“…In rabbits infected with T. evansi IgG antibodies were first detected in the serum seven DPI (UCHE et al, 1993). Results from the present study differ, however, from other reports using both IFAT and ELISA in sera of rabbits (LUCKINS et al, 1978), camels (LUCKINS et al, 1979) and dogs (AQUINO et al, 1999) in which antibodies were revealed within 10 to 19 DPI.…”

Section: Resultscontrasting

confidence: 96%

“…The immune system plays a crucial role in the outcome of any parasite challenge (UCHE et al, 1993). Studies on the antibody response in horses infected with T. evansi are limited, what justifies greater efforts in the search and improvement of diagnostic methods for this trypanosomiasis that constitutes an important problem in horse-raising system.…”

Section: Introductionmentioning

confidence: 99%

Resposta imune humoral de eqüinos infectados experimentalmente com Trypanosoma evansi

et al. 2001

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Resumo: Seis eqüinos foram inoculados com 10 6 tripomastigota sangüícolas de Trypanosoma evansi. Três outros animais foram mantidos como testemunhas. Amostras de soro sangüíneo foram obtidas de todos os animais, antes da inoculação, e diariamente até o 14º dia pós inoculação (DPI); após este período uma vez por semana. Pesquisa de anticorpos anti-T. evansi, foram realizadas através da reação de imunofluorescência indireta (RIFI) e do ensaio de imunoabsorção enzimática (ELISA). A resposta imune humoral, detectada através da RIFI e do ELISA, iniciou-se, em média, a partir do oitavo DPI, alcançando títulos máximos após quatro semanas de evolução, e os titulos de anticorpos anti-T. evansi mantiveram-se elevadas até o término das observações. Palavras-chave: Trypanosoma evansi, tripanossomiase, eqüinos, resposta imune.Abstract: Six adult horses were experimentally infected with Trypanosoma evansi (10 6 parasites). Three other adult horses served as negative control. Serum samples of the experimentally infected horses with T. evansi and non-infected controls horses were obtained before inoculation, and daily thereafter until 14 days post infection (DPI). After that time the serum samples were obtained weekly. Sera of the infected and non-infected control horses was tested by indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against T. evansi. Both ELISA and IFAT detected trypanosomal antibodies shortly after infection and showed progressive increases in antibodies levels during early stages of infection. The responses started on the eighth and eleventh DPI. Maximum IFAT and ELISA values were reached after four weeks of infection and were maintained at this level until the end of the period of study.

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Trypanosomosis research at the centre for tropical veterinary medicine (CTVM) 1970 to 1995

Boid

Hunter

Jones

et al. 1996

Trop Anim Health Prod

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This review covers aspects of research work carried out on animal trypanosomes at the Centre for Tropical Veterinary Medicine (CTVM) during the last 25 years. The review covers work on antigenic variation, tissue culture, drug resistance, immunology, biochemistry and pathology of Trypanosoma brucei, T. congolense, T. gambiense and T. evansi. It is not intended as an exhaustive review of the subject but focuses on certain aspects of these areas which are presented in relation to work carried out within the broader scientific community.

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Complement (C3) levels and activation in rabbits experimentally infected withTrypanosoma evansi

Cited by 8 publications

References 11 publications

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Antigenic characterization of Trypanosoma evansi using sera from experimentally and naturally infected bovines, equines, dogs, and coatis

Resposta imune humoral de eqüinos infectados experimentalmente com Trypanosoma evansi

Trypanosomosis research at the centre for tropical veterinary medicine (CTVM) 1970 to 1995

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