Complement-dependent antiviral monospecific antibody-mediated lysis of murine cells coated with Sendai virus or its envelope component

Hosaka, Yasuhiro; Fukami, Yasuo; Yasuda, Yuko; Bonilla, J. Alfredo

doi:10.1128/iai.27.2.355-363.1980

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…Figure 11 also illustrates possible fracture planes of cell associations with LLC-SV and TE. We could not find any morphological evidence of envelope fusion with LLC-SV or TE, which is consistent with the finding that they have no hemolytic or cell fusion activity (9,13,30).…”

Section: Envelope Fusion With Repsupporting

confidence: 90%

“…In a previous report (13), we showed that TE-coated but not LLC-SV-coated murine cells were susceptible to complement-dependent antibody-mediated cytolysis. The present results suggest the formation of a no more stable membrane association with TE than with LLC-SV.…”

Section: Cell Association With Llc-sv and Tementioning

confidence: 68%

“…REP has these activities (16), and although much evidence to support the envelope fusion by REP has been obtained (6,7,(32)(33)(34), no direct morphological evidence has been reported so far. TE were depleted of the bulk of lipids during the preparation and had no hemolytic or cell fusion activity, but TE-coated cells were susceptible to complement-dependent antibody-mediated cytolysis (CDAMC) (13).…”

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confidence: 99%

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Fusion of Sendai Viruses or Subviral Envelope Components with Chicken Erythrocytes Observed by Freeze‐Fracture Electron Microscopy

Hosaka

Yasuda

Fukai

et al. 1983

Microbiology and Immunology

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Four different types of envelope of Sendai virus or subviral components, that is, infectious and non-infectious virions, reassembled envelope particles (REP), and Tween-ether-treated envelope fragments (TE), were studied comparatively for membrane interactions with chicken erythrocytes by freeze-fracture electron microscopy, specifically for membrane alteration by envelope fusion.The freeze-fracture replicas of the attachment of the four envelopes in the cold exhibited a common pattern of impressions with attached envelopes, although the fracture plane traversed from erythrocyte to envelope at the periphery of the contact areas of three of the envelopes but not of TE, where the fracture plane mostly cut only through erythrocyte membranes impressed with TE. The freeze-fracture replicas of the four envelopes reacting with erythrocytes after a short incubation period at 37 C exhibited distinctive features: infectious virions and REP displayed evidence of envelope fusion, but non-infectious virions and TE showed a particular pattern of envelope association without fusion. Our data demonstrate that the pattern specific for envelope fusion is the formation of a continuous membrane from envelope to cell membrane in a cross fracture of an erythrocyte.Although envelope fusion by Sendai virions has been extensively studied by electron microscopy (1, 3, 4, 18, 20-28, 31, 36), the morphology of membrane interactions of subviral envelope particles with cell membranes as well as those of non-infectious Sendai virions (9, 30) with host cells is not yet well elucidated. Using Sendai virions and subviral envelope components, which were different from each other in their ability to affect membranes, we wanted to observe membrane alterations during the interactions of these envelopes with cell membranes and to charaterize the membrane changes specific for envelope fusion. The envelopes we used were infectious and non-infectious virions, reassembled envelope particles (REP) from Nonidet P40 (NP40)-solubilized virus envelopes (16), and Tween-ether-treated envelope fragments (TE) (15). Non-infectious Sendai virions, which contain a precursor fusion protein (9, 30), do not have hemolytic or cell fusion activity (8, 11). REP has these activities (16), and although much evidence 25

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Section: Envelope Fusion With Repsupporting

confidence: 90%

Section: Cell Association With Llc-sv and Tementioning

confidence: 68%

mentioning

confidence: 99%

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Fusion of Sendai Viruses or Subviral Envelope Components with Chicken Erythrocytes Observed by Freeze‐Fracture Electron Microscopy

Hosaka

Yasuda

Fukai

et al. 1983

Microbiology and Immunology

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“…Non-infectious Sendai virus was grown in LLC-MK2 cells, an established line of rhesus monkey kidney cells, which had been infected with egg-grown infectious virus. This virus (LLC-SV) had a log ratio of infectivity to hemagglutination units of about 2, in contrast to infectious virus which had a ratio of 6 to 7, and it contained predominantly precursor protein Fo (9).…”

Section: Methodsmentioning

confidence: 95%

“…Hale et al (6), using MDBK cell-grown noninfectious Sendai virus, reported that virusattached P815 and EIA cells absorbed anti-Sendai virus antibodies as efficiently as did trypsinactivated infectious virus-coated cells. However, noninfectious Sendai virus-coated cells were found to be resistant to anti-Sendai cytotoxic T lymphocyte-mediated lysis (4,5) and also, to complement-dependent antibody-mediated cytolysis (9). Therefore, it was interesting to elucidate the ultrastructure of these nonfunctional virus-cell associations.…”

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confidence: 99%

Immunoelectron microscopic study on interactions of noninfectious sendai virus and murine cells

Yasuda¹,

Hosaka²,

Fukami³

et al. 1981

J Virol

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The early interactions of LLC-MK2 cell-grown noninfectious Sendai virus and a murine cell line, P815 mastocytoma ascitic cells, were studied by electron microscopy, using the ferritin-conjugated antibody technique with anti-virus glycoprotein serum. For comparison, the interactions of egg-grown infectious Sendai virus with the same cells were also examined. When noninfectious virus was adsorbed to the cells in the cold, the cell membranes become partially invaginated at the site of contact of adsorbed virions, but ferritin-conjugated antibodies did not penetrate into the areas of envelope-cell membrane association. This pattern of virus attachment was similar to that of infectious virus attachment. Upon subsequent incubation at 37°C, most of the adsorbed noninfectious virions were taken into cytoplasmic vesicles and then degraded, although a few virions remained attached to the cell membrane. No evidence of fusion of envelopes of noninfectious virions was obtained. On the other hand, envelopes of infectious virions fused with the cell membrane, and the transferred viral antigens diffused on the cell surfaces and then decreased in number.

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Separation of Sendai virus glycoproteins by using glutaraldehyde-treated erythrocytes and preparation of monospecific antisera against the glycoproteins

Hosaka¹

1980

Infect Immun

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Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins, F and HN, were separated from Triton X-100- or Nonidet P-40-solubilized envelopes as unadsorbed and eluted fractions, respectively, by using glutaralde-hyde-treated chicken erythrocytes. These separated glycoproteins were biologically active. Monospecific antisera (in terms of monoreactivity to virus glycoproteins in gel diffusion precipitation patterns) were prepared by using these fractions as immunogens. Anti-HN rabbit serum inhibited all of the viral activities tested (infectivity, neuraminidase, hemagglutinating, and viral hemolysis), whereas anti-F serum definitely inhibited viral hemolysis only, although the two antisera enhanced neutralization in the presence of complement. The advantages and disadvantages of this separation method were discussed.

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Complement-dependent antiviral monospecific antibody-mediated lysis of murine cells coated with Sendai virus or its envelope component

Cited by 4 publications

References 28 publications

Fusion of Sendai Viruses or Subviral Envelope Components with Chicken Erythrocytes Observed by Freeze‐Fracture Electron Microscopy

Fusion of Sendai Viruses or Subviral Envelope Components with Chicken Erythrocytes Observed by Freeze‐Fracture Electron Microscopy

Immunoelectron microscopic study on interactions of noninfectious sendai virus and murine cells

Separation of Sendai virus glycoproteins by using glutaraldehyde-treated erythrocytes and preparation of monospecific antisera against the glycoproteins

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