Complement-resistant Moraxella catarrhalis forms a genetically distinct lineage within the species

Verduin, C. M.

doi:10.1016/s0378-1097(00)00010-0

Cited by 16 publications

(33 citation statements)

References 25 publications

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“…Recently, the existence of these two lineages was further confirmed by Wirth et al via multilocus sequence typing (MLST) (153). The first of these two phylogenetic lineages, known as the serosensitive lineage, is characterized by moderate virulence, and is enriched for strains that are sensitive to complement-mediated killing (19,148). The other, more virulent lineage, the so-called seroresistant lineage, contains predominantly complement-resistant strains (19,148) and is enriched for strains with the ability to adhere to epithelial cells (19).…”

Section: M35 Porinmentioning

confidence: 98%

“…These results suggest that M35 is a general porin important for the uptake of essential nutrients, especially under nutrient-poor conditions. (19,116,126,148,151,153). Early molecular epidemiological studies indicated that M. catarrhalis isolates are genetically diverse and revealed that there are actually two distinct phylogenetic lineages (19,59,126,148,153).…”

Section: M35 Porinmentioning

confidence: 99%

“…The first of these two phylogenetic lineages, known as the serosensitive lineage, is characterized by moderate virulence, and is enriched for strains that are sensitive to complement-mediated killing (19,148). The other, more virulent lineage, the so-called seroresistant lineage, contains predominantly complement-resistant strains (19,148) and is enriched for strains with the ability to adhere to epithelial cells (19). The seroresistant lineage predominantly harbors isolates of 16S rRNA type 1, whereas 16S rRNA type 2/3 isolates group within the serosensitive lineage (19,150).…”

Section: M35 Porinmentioning

confidence: 99%

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Molecular Aspects ofMoraxella catarrhalisPathogenesis

Vries

Bootsma

Hays

et al. 2009

Microbiol Mol Biol Rev

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SUMMARY In recent years, Moraxella catarrhalis has established its position as an important human mucosal pathogen, no longer being regarded as just a commensal bacterium. Further, current research in the field has led to a better understanding of the molecular mechanisms involved in M. catarrhalis pathogenesis, including mechanisms associated with cellular adherence, target cell invasion, modulation of the host's immune response, and metabolism. Additionally, in order to be successful in the host, M. catarrhalis has to be able to interact and compete with the commensal flora and overcome stressful environmental conditions, such as nutrient limitation. In this review, we provide a timely overview of the current understanding of the molecular mechanisms associated with M. catarrhalis virulence and pathogenesis.

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Section: M35 Porinmentioning

confidence: 98%

Section: M35 Porinmentioning

confidence: 99%

Section: M35 Porinmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Aspects ofMoraxella catarrhalisPathogenesis

Vries

Bootsma

Hays

et al. 2009

Microbiol Mol Biol Rev

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“…PFGE was performed as detailed by Verduin et al (2000). Digestions were performed using SpeI at 20 U per reaction and the following electrophoresis protocol: the first block consisted of a constant voltage of 6 V cm…”

Section: Methodsmentioning

confidence: 99%

Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001–2002

et al. 2008

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Moraxella catarrhalis is generally associated with upper respiratory tract infections in children and lower respiratory tract infections in adults. However, little is known regarding the population biology of isolates infecting these two age groups. To address this, a population-screening strategy was employed to investigate 195 worldwide M. catarrhalis isolates cultured from children (,5 years of age) and adults (.20 years of age) presenting with respiratory disease in the years [2001][2002]. Parameters compared included: genotype analysis; autoagglutination/biofilmforming ability; serum resistance; uspA1, uspA2, uspA2H, hag and mcaP incidence; copB/LOS/ ompCD/16S rRNA types; and UspA1/Hag expression. A significant difference in biofilm formation (P50.002), but not in autoagglutination or serum resistance, was observed, as well as significant differences in the incidence of uspA2-and uspA2H-positive isolates, and the distribution of lipooligosaccharide (LOS) types (P,0.0001 and P50.01, respectively). Further, a significant decrease in the incidence of Hag expression (for isolates possessing the hag gene) was observed in adult isolates (P50.001). Both uspA2H and LOS type B were associated with 16S rRNA type 1 isolates only, and two surrogate markers (copB and ompCD PCR RFLP types) for the two major M. catarrhalis 16S rRNA genetic lineages were identified. In conclusion, there are significant differences in phenotype and gene incidence between M. catarrhalis isolates from children and adults presenting with respiratory disease, possibly as a result of immune evasion in the adult age group. Our results should also be useful in the choice of effective vaccine candidates against M. catarrhalis.

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“…Genotyping of M. catarrhalis isolates was performed using PFGE and multilocus sequence typing (MLST). PFGE was also performed on H. influenzae isolates.PFGE for M. catarrhalis was performed as detailed by Verduin et al (2000). Briefly, M. catarrhalis plug digestions were performed using SpeI at 20 U per reaction and an electrophoresis protocol comprising a first block with a constant voltage of 6 V cm 21 and a pulse time from 3.5 to 25 s during the first 12 h, followed by a second block of 8 h where the pulse time increased linearly from 1 to 5 s. The PFGE protocol for H. influenzae was adapted from the work of Moor et al (1999).…”

mentioning

confidence: 99%

Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae

et al. 2011

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The colonization dynamics of Moraxella catarrhalis were studied in a population comprising 1079 healthy children living in Rotterdam, The Netherlands (the Generation R Focus cohort). A total of 2751 nasal swabs were obtained during four clinic visits timed to take place at 1.5, 6, 14 and 24 months of age, yielding a total of 709 M. catarrhalis and 621 Haemophilus influenzae isolates. Between January 2004 and December 2006, approximate but regular 6-monthly cycles of colonization were observed, with peak colonization incidences occurring in the autumn/winter for M. catarrhalis, and winter/spring for H. influenzae. Co-colonization was significantly more likely than single-species colonization with either M. catarrhalis or H. influenzae, with genotypic analysis revealing no clonality for co-colonizing or single colonizers of either bacterial species. This finding is especially relevant considering the recent discovery of the importance of H. influenzae-M. catarrhalis quorum sensing in biofilm formation and host clearance. Bacterial genotype heterogeneity was maintained over the 3-year period of the study, even within this relatively localized geographical region, and there was no association of genotypes with either season or year of isolation. Furthermore, chronological and genotypic diversity in three immunologically important M. catarrhalis virulence genes (uspA1, uspA2 and hag/mid ) was also observed. This study indicates that genotypic variation is a key factor contributing to the success of M. catarrhalis colonization of healthy children in the first years of life. Furthermore, variation in immunologically relevant virulence genes within colonizing populations, and even within genotypically identical M. catarrhalis isolates, may be a result of immune evasion by this pathogen. Finally, the factors facilitating M. catarrhalis and H. influenzae co-colonization need to be further investigated. INTRODUCTIONMoraxella catarrhalis is part of the normal bacterial flora in the nasopharynx of children, although over the past two decades, it has emerged as a significant bacterial pathogen and not simply a commensal colonizer (Verduin et al., 2002). Studies have shown that the bacterium rapidly colonizes the nasopharynx soon after birth and that the carriage rate of M. catarrhalis in healthy children varies between 7 and 36 % (Verhaegh et al., 2010). However, in children with upper respiratory tract infections (URTI), including acute otitis media (AOM), the carriage rate increases to approximately 50 % (Berner et al., 1996;Konno et al., 2006;Pettigrew et al., 2008). Otitis media itself is a particularly important URTI during early Abbreviations: AOM, acute otitis media; CEACAM, carcinoembryonic antigen-related cell adhesion molecule; LOS, lipooligosaccharide; MLST, multilocus sequence typing; OMP, outer-membrane protein; OR, odds ratio; OMV, outer-membrane vesicle; URTI, upper respiratory tract infection. childhood and the primary reason for children to visit a physician (Freid et al., 1998;Plasschaert et al., 2006). Furt...

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Complement-resistant Moraxella catarrhalis forms a genetically distinct lineage within the species

Cited by 16 publications

References 25 publications

Molecular Aspects ofMoraxella catarrhalisPathogenesis

Molecular Aspects ofMoraxella catarrhalisPathogenesis

Age-related genotypic and phenotypic differences in Moraxella catarrhalis isolates from children and adults presenting with respiratory disease in 2001–2002

Colonization of healthy children by Moraxella catarrhalis is characterized by genotype heterogeneity, virulence gene diversity and co-colonization with Haemophilus influenzae

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