Complementary DNA cloning, sequencing and expression of an unusual dehydrin from blueberry floral buds

Levi, Amnon; Panta, Ganesh R.; Parmentier, Cécile; Muthalif, Mubarack M.; Arora, Rajeev; Shanker, Savita; Rowland, Lisa J.

doi:10.1034/j.1399-3054.1999.100114.x

Cited by 49 publications

(29 citation statements)

References 37 publications

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“…There is also recent evidence, not previously reported, that some dehydrins such as DHN-like proteins from blueberry (Vaccinium smpp.) and Pistacia vera L. can be glycosylated [61,62]. One of the blueberry DHNs has been cloned, and the gene was found to encode a K5 DHN [62].…”

Section: Post-translational Modificationsmentioning

confidence: 99%

Plant dehydrins — Tissue location, structure and function

Rorat

2006

Cellular and Molecular Biology Letters

310

228

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Dehydrins (DHNs) are part of a large group of highly hydrophilic proteins known as LEA (Late Embryogenesis Abundant). They were originally identified as group II of the LEA proteins. The distinctive feature of all DHNs is a conserved, lysine-rich 15-amino acid domain, EKKGIMDKIKEKLPG, named the K-segment. It is usually present near the C-terminus. Other typical dehydrin features are: a track of Ser residues (the S-segment); a consensus motif, T/VDEYGNP (the Y-segment), located near the N-terminus; and less conserved regions, usually rich in polar amino acids (the Φ-segments). They do not display a well-defined secondary structure. The number and order of the Y-, S-and K-segments define different DHN sub-classes: Y n SK n , Y n Kn, SKn, K n and K n S. Dehydrins are distributed in a wide range of organisms including the higher plants, algae, yeast and cyanobacteria. They accumulate late in embryogenesis, and in nearly all the vegetative tissues during normal growth conditions and in response to stress leading to cellular dehydration (e.g. drought, low temperature and salinity). DHNs are localized in different cell compartments, such as the cytosol, nucleus, mitochondria, vacuole, and the vicinity of the plasma membrane; however, they are primarily localized to the cytoplasm and nucleus. The precise function of dehydrins has not been established yet, but in vitro experiments revealed that some DHNs (YSK n -type) bind to lipid vesicles that contain acidic phospholipids, and others (K n S) were shown to bind metals and have the ability to scavenge hydroxyl radicals [Asghar, R. et al. Protoplasma 177 (1994)

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Section: Post-translational Modificationsmentioning

confidence: 99%

Plant dehydrins — Tissue location, structure and function

Rorat

2006

Cellular and Molecular Biology Letters

310

228

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“…High levels of DHN in buds during winter have been reported for many herbaceous and woody species (GolanGoldhirsh and Shachak 1998; Levi et al 1999;Rinne et al 1999;Wisniewski et al 1999). Further, the levels of a DHN-like protein are at their highest during winter and decline in spring upon spermatogenesis and Xowering in deciduous species (Golan-Goldhirsh and Shachak 1998).…”

Section: Expression Of Dhn Genes Of Norway Sprucementioning

confidence: 85%

Dehydrins expression related to timing of bud burst in Norway spruce

Yakovlev¹,

Asante

Fossdal³

et al. 2008

Planta

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Cold deacclimation and preparation to flushing likely requires rehydration of meristems. Therefore, water stress related genes, such as dehydrins (DHN), might play an important role in providing protection during winter dormancy, deacclimation and bud burst timing processes. Here we report the sequence analysis of several Norway spruce DHN identified in late and early flushing suppressive subtraction hybridization cDNA libraries and in our Norway spruce EST database. We obtained 15 cDNAs, representing eight genes from three distinct types of DHN, and studied differential expression of these genes before and during bud burst in spring, using qRT-PCR. We found the visible reduction in transcript level of most DHN towards the bud burst, supported by a significant down-regulation of the DHN in needles during experimental induction of bud burst applied at three time points during autumn in Norway spruce grafts. For most of the DHN transcripts, their expression levels in late-flushing spruces were significantly higher than in the early flushing ones at the same calendar dates but were remarkably similar at the same bud developmental stage. From our results we may conclude that the difference between the early and the late families is in timing of the molecular processes leading to bud burst due to differences in their response to the increasing temperature in the spring. They are induced much earlier in the early flushing families.

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“…5 and 6), revealing that LT induces a distinct pathway, which is independent of action of phyA. Earlier studies have shown that DHNs accumulate in woody plants in response to SD (Welling et al, 1997;Rinne et al, 1998) and LT (Levi et al, 1999;Richard et al, 2000). We were able to show that these environmental cues induce DHN gene expression independently.…”

Section: Discussionmentioning

confidence: 99%

Independent Activation of Cold Acclimation by Low Temperature and Short Photoperiod in Hybrid Aspen

et al. 2002

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Temperate zone woody plants cold acclimate in response to both short daylength (SD) and low temperature (LT). We were able to show that these two environmental cues induce cold acclimation independently by comparing the wild type (WT) and the transgenic hybrid aspen (Populus tremula ϫ Populus tremuloides Michx.) line 22 overexpressing the oat (Avena sativa) PHYTOCHROME A gene. Line 22 was not able to detect the SD and, consequently, did not stop growing in SD conditions. This resulted in an impaired freezing tolerance development under SD. In contrast, exposure to LT resulted in cold acclimation of line 22 to a degree comparable with the WT. In contrast to the WT, line 22 could not dehydrate the overwintering tissues or induce the production of dehydrins (DHN) under SD conditions. Furthermore, abscisic acid (ABA) content of the buds of line 22 were the same under SD and long daylength, whereas prolonged SD exposure decreased the ABA level in the WT. LT exposure resulted in a rapid accumulation of DHN in both the WT and line 22. Similarly, ABA content increased transiently in both the WT and line 22. Our results indicate that phytochrome A is involved in photoperiodic regulation of ABA and DHN levels, but at LT they are regulated by a different mechanism. Although SD and LT induce cold acclimation independently, ABA and DHN may play important roles in both modes of acclimation.Cold acclimation capacity is much higher in temperate zone woody plants compared with herbaceous species. Herbaceous plants survive normally under an insulating snow cover and a moderate low temperature (LT) tolerance is sufficient for survival. However, trees have to be able to face extremes of temperature and light conditions, and because of their long generation time and age, a high capacity for cold acclimation is paramount for their survival. The extreme freezing tolerance of woody plants is achieved by sequential stages of cold acclimation of which the first is initiated by short daylength (SD) and second and third by LT and freezing temperatures, respectively (Weiser, 1970). Although recent breakthroughs have increased our knowledge of the molecular basis of frost hardiness in herbaceous species, which acclimate primarily in response to LT (Thomashow, 1999), very little is known about cold acclimation of woody plants.Phytochromes are the photoreceptors responsible for photoperiod detection in plants. Photosignal per-

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Complementary DNA cloning, sequencing and expression of an unusual dehydrin from blueberry floral buds

Cited by 49 publications

References 37 publications

Plant dehydrins — Tissue location, structure and function

Plant dehydrins — Tissue location, structure and function

Dehydrins expression related to timing of bud burst in Norway spruce

Independent Activation of Cold Acclimation by Low Temperature and Short Photoperiod in Hybrid Aspen

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