Complementary expression of melanosomal antigens and constant expression of pigment-independent antigen during the evolution of melanocytic tumours

Takahashi, Hiroyuki; Parsons, Peter G.; Favier, D.; McEwan, Max; Strutton, Geoffrey; Akutsu, Yutaka; Jimbow, Kowichi

doi:10.1007/bf01600302

Cited by 5 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Essentially immunohistochemistry was performed as described (36). Briefly, melanocytes were plated out at 5 × 10 4 cells/well in 24‐well tissue culture plates containing a coverslip and allowed to grow for 48 h. Coverslips were fixed in 4% paraformaldehyde, blocked in 3% FCS and primary antibody added at 200 μl/well anti‐intermediary filament antigen ( IFA) (37), anti‐TYR C513 (38), anti‐tyrosinase‐related protein‐1 (TYRP1) B8G3 (39) and anti‐7H11 (40) monoclonal supernatants without dilution, and HMB45 (Biogenex, San Ramon, CA, USA) and chromogranin‐A (CHM) (Biogenex, San Ramon, CA, USA) diluted 1:250 in blocking solution. Secondary antibody was a 1:500 dilution of anti‐mouse‐conjugated Alexa594 (Molecular Probes, Eugene, OR, USA).…”

Section: Immunofluorescencementioning

confidence: 99%

Screening of Human Primary Melanocytes of Defined Melanocortin‐1 Receptor Genotype: Pigmentation Marker, Ultrastructural and UV‐Survival Studies

Leonard

Marks

Chen

et al. 2003

Pigment Cell Research

Self Cite

View full text Add to dashboard Cite

Recent population studies have demonstrated an association with the red-hair and fair-skin phenotype with variant alleles of the melanocortin-1 receptor (MC1R) which result in amino acid substitutions within the coding region leading to an altered receptor activity. In particular, Arg151Cys, Arg160Trp and Asp294His were the most commonly associated variants seen in the south-east Queensland population with at least one of these alleles found in 93% of those with red hair. In order to study the individual effects of these variants on melanocyte biology and melanocytic pigmentation, we established a series of human melanocyte strains genotyped for the MC1R receptor which included wild-type consensus, variant heterozygotes, compound heterozygotes and homozygotes for Arg151Cys, Arg160Trp, Val60Leu and Val92Met alleles. These strains ranged from darkly pigmented to amelanotic, with all strains of consensus sequence having dark pigmentation. UV sensitivity was found not to be associated with either MC1R genotype or the level of pigmentation with a range of sensitivities seen across all genotypes. Ultrastructural analysis demonstrated that while consensus strains contained stage IV melanosomes in their terminal dendrites, Arg151Cys and Arg160Trp homozygote strains contained only stage II melanosomes. This was despite being able to show expression of tyrosinase and tyrosinase-related protein-1 markers, although at reduced levels and an ability to convert exogenous 3,4-dihydroxyphenyl-alanine (DOPA) to melanin in these strains.

show abstract

Section: Immunofluorescencementioning

confidence: 99%

Screening of Human Primary Melanocytes of Defined Melanocortin‐1 Receptor Genotype: Pigmentation Marker, Ultrastructural and UV‐Survival Studies

Leonard

Marks

Chen

et al. 2003

Pigment Cell Research

Self Cite

View full text Add to dashboard Cite

show abstract

Clear cell (“sugar”) tumor of the lung is a lesion strictly related to angiomyolipoma— The concept of a family of lesions characterized by the presence of the perivascular epithelioid cells (PEC)

et al. 1994

View full text Add to dashboard Cite

Determination of proliferating fractions in malignant melanomas by anti‐PCNA/cyclin monoclonal antibody

Takahashi

Strutton²,

Parsons

1991

Histopathology

View full text Add to dashboard Cite

An immunohistochemical study of melanocytic tumours using 19A2, a monoclonal antibody against proliferating cell nuclear antigen (PCNA/cyclin), was performed on tissues routinely processed with formalin fixation and paraffin embedding. In normal skin, keratinocytes of the suprabasal region in epidermis, the papillae and outer root sheath of hair follicles and the basal cells lining the lobules of sebaceous glands were stained in the nucleus. Other skin components, including basal and follicular melanocytes, did not demonstrate nuclear labelling. In addition, expression of PCNA/cyclin in keratinocytes was higher in sun-exposed skin compared with unexposed skin. In melanocytic lesions, PCNA/cyclin positive tumour cells increased in number and staining intensity according to the following progression: common melanocytic naevi; dysplastic naevi; primary melanomas; and metastatic melanomas. Expression of PCNA/cyclin, therefore, provides a useful marker for proliferation and tumour progression in skin.

show abstract

Complementary expression of melanosomal antigens and constant expression of pigment-independent antigen during the evolution of melanocytic tumours

Cited by 5 publications

References 25 publications

Screening of Human Primary Melanocytes of Defined Melanocortin‐1 Receptor Genotype: Pigmentation Marker, Ultrastructural and UV‐Survival Studies

Screening of Human Primary Melanocytes of Defined Melanocortin‐1 Receptor Genotype: Pigmentation Marker, Ultrastructural and UV‐Survival Studies

Clear cell (“sugar”) tumor of the lung is a lesion strictly related to angiomyolipoma— The concept of a family of lesions characterized by the presence of the perivascular epithelioid cells (PEC)

Determination of proliferating fractions in malignant melanomas by anti‐PCNA/cyclin monoclonal antibody

Contact Info

Product

Resources

About