Complementary liver and serum protein profile in wild boars infected by the giant liver fluke Fascioloides magna using tandem mass tags quantitative approach

Kuleš, Josipa; Lovrić, Lea; Gelemanović, Andrea; Ljubić, Blanka Beer; Rubić, Ivana; Bujanić, Miljenko; Konjević, Dean

doi:10.1016/j.jprot.2021.104332

Cited by 12 publications

(15 citation statements)

References 86 publications

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“…tegumental and secreted proteins and extracellular vesicles), by detecting parasite proteins in its host, or by defining the infection effects on the host's proteome. Thus far, significant progress has been achieved in understanding critical high-impact zoonotic and animal parasitic diseases through this technology [20][21][22][23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

A bloody interaction: plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii

et al. 2022

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Background Sparicotylosis is an enzootic parasitic disease that is well established across the Mediterranean Sea. It is caused by the polyopisthocotylean monogenean Sparicotyle chrysophrii and affects the gills of gilthead sea bream (GSB; Sparus aurata). Current disease management, mitigation and treatment strategies are limited against sparicotylosis. To successfully develop more efficient therapeutic strategies against this disease, understanding which molecular mechanisms and metabolic pathways are altered in the host is critical. This study aims to elucidate how S. chrysophrii infection modulates the plasma proteome of GSB and to identify the main altered biological processes involved. Methods Experimental infections were conducted in a recirculating aquaculture system (RAS) in which naïve recipient GSB ([R]; 70 g; n = 50) were exposed to effluent water from S. chrysophrii-infected GSB (98 g; n = 50). An additional tank containing unexposed naïve fish (control [C]; 70 g; n = 50) was maintained in parallel, but with the open water flow disconnected from the RAS. Haematological and infection parameters from sampled C and R fish were recorded for 10 weeks. Plasma samples from R fish were categorised into three different groups according to their infection intensity, which was based on the number of worms fish−1: low (L: 1–50), medium (51–100) and high (H: > 100). Five plasma samples from each category and five C samples were selected and subjected to a SWATH-MS proteome analysis. Additional assays on haemoglobin, cholesterol and the lytic activity of the alternative complement pathway were performed to validate the proteome analysis findings. Results The discriminant analysis of plasma protein abundance revealed a clear separation into three groups (H, M/L and C). A pathway analysis was performed with the differentially quantified proteins, indicating that the parasitic infection mainly affected pathways related to haemostasis, the immune system and lipid metabolism and transport. Twenty-two proteins were significantly correlated with infection intensity, highlighting the importance of apolipoproteins, globins and complement component 3. Validation assays of blood and plasma (haemoglobin, cholesterol and lytic activity of alternative complement pathway) confirmed these correlations. Conclusions Sparicotylosis profoundly alters the haemostasis, the innate immune system and the lipid metabolism and transport in GSB. This study gives a crucial global overview of the pathogenesis of sparicotylosis and highlights new targets for further research. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

A bloody interaction: plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii

et al. 2022

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“…For the high-resolution LC-MS/MS analysis of TMT-labelled peptides, the protocol from previous studies (Kuleš et al. 2021 ) was followed. The Ultimate 3000 RSLCnano instrument (Dionex, Germering, Germany) was used for the chromatographic separation.…”

Section: Methodsmentioning

confidence: 99%

“…), following the detailed instructions by Kuleš et al. ( 2021 ). The official gene symbols replaced the UniProtKB identifiers via the UniProtKB ID mapping tool ( https://www.uniprot.org/uploadlists/ ), which allowed the Gene Ontology (GO) classification, using the Protein Analysis Through Evolutionary Relationship (PANTHER) tool ( http://www.pantherdb.org/ ) with the subset of GO terms (GO Slim database) (Mi et al.…”

Section: Methodsmentioning

confidence: 99%

Profiling the alterations of serum proteome in dairy cows with retained placenta using high-throughput tandem mass tags quantitative approach

Beletić

Kuleš

Maslov

et al. 2023

Veterinary Quarterly

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Background Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production. Aim To investigate the difference in the serum proteome between the cows with RP and the physiologic puerperium (PP). Material & Methods Analysis of serum samples from nine cows with RP and six with PP using high-resolution liquid chromatography-tandem mass spectrometry approach. The proteins differing in the relative abundance between the PP and RP groups were classified using the Protein Analysis Through Evolutionary Relationship tool. For the pathway enrichment analysis, the REACTOME tool, with the human genome as the background, was employed. The criterion for significance was the false discovery rate corrected P-value less than 0.05. Results In total 651 proteins were identified with altered relative abundance of ten proteins. Among them, seven had higher, and three showed lower relative abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in lipoprotein metabolism, and the remaining ones associated with for instance redox homeostasis, post-translational modification, and scavenging. Finally, the validation of the proteomic results showed that haptoglobin and lipopolysaccharide-binding protein levels reliably differentiated between the RP and PP groups. Conclusion The pattern of serum proteome alterations in the cows with RP mirrored several interplaying mechanisms underlying the systematic response to the presence of RP, therefore representing a source to mine for predictive or prognostic biomarkers.

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“…These interactions can be studied either by determining the expression of the parasite proteome throughout the infection (i.e., tegumental and secreted proteins and extracellular vesicles), by detecting parasite proteins in its host, or by de ning the infection effects on the host's proteome. Thus far, signi cant progress has been achieved in understanding critical high-impact zoonotic and animal parasitic diseases through this technology [20][21][22][23][24][25][26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

A bloody interaction: Plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii

Riera-Ferrer

Piazzon

Pozo

et al. 2022

Preprint

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Background:Sparicotylosis is an endemic parasitic disease across the Mediterranean Sea caused by the polyopisthocotylean monogenean Sparicotyle chrysophrii, which affects the gills of gilthead sea bream (GSB; Sparus aurata). Current disease-management, mitigation and treatment strategies are scarce against sparicotylosis. In order to successfully develop more efficient therapeutic strategies against this disease, understanding which molecular mechanisms and metabolic pathways are altered in the host is critical. This study aims to elucidate how S. chrysophrii infection modulates GSB physiological status and to identify the main altered biological processes through plasma proteomics.Methods:Experimental infections were conducted in a recirculating aquaculture system (RAS), exposing naïve recipient GSB (R; 70 g; N= 50) to effluent water from S. chrysophrii-infected GSB. An additional tank with unexposed naïve fish (C; 70 g; N=50) was maintained in parallel, with open water flow disconnected from the RAS. Haematological and infection parameters from sampled C and R fish were registered for 10 weeks. Plasma samples from R fish were categorised into 3 different groups according to their infection intensity in the right-sided gill arches: low, medium and high (L: 1-25; M: 26-50; H: >50 worms, respectively). Five plasma samples of each category were selected, in addition to five C samples and underwent a SWATH-MS proteome analysis. Additional assays on haemoglobin, cholesterol and the lytic activity of the alternative complement pathway were performed to validate the proteome analysis findings.Results:The discriminant analysis of the plasma protein abundance revealed a clear separation into 3 groups (H, M/L and C). A pathway analysis was performed with the differentially quantified proteins, indicating that the parasitic infection mainly affected pathways related to haemostasis, the immune system and lipid metabolism and transport.Twenty-two proteins significantly correlated with the infection intensity, highlighting apolipoproteins, globins and complement c3. Validation assays in blood and plasma (haemoglobin, cholesterol and lytic activity of alternative complement pathway) confirmed these correlations.Conclusions:Sparicotylosis profoundly alters the haemostasis, the innate immune system and the lipid metabolism and transport in GSB. This study gives a crucial global overview of the pathogenesis of sparicotylosis and highlights new targets for further research.

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Complementary liver and serum protein profile in wild boars infected by the giant liver fluke Fascioloides magna using tandem mass tags quantitative approach

Cited by 12 publications

References 86 publications

A bloody interaction: plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii

A bloody interaction: plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii

Profiling the alterations of serum proteome in dairy cows with retained placenta using high-throughput tandem mass tags quantitative approach

A bloody interaction: Plasma proteomics reveals gilthead sea bream (Sparus aurata) impairment caused by Sparicotyle chrysophrii

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