2009
DOI: 10.1074/mcp.m900013-mcp200
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Complementary Quantitative Proteomics Reveals that Transcription Factor AP-4 Mediates E-box-dependent Complex Formation for Transcriptional Repression of HDM2>

Abstract: Transcription factor activating enhancer-binding protein 4 (AP-4) is a basic helix-loop-helix protein that binds to E-box elements. AP-4 has received increasing attention for its regulatory role in cell growth and development, including transcriptional repression of the human homolog of murine double minute 2 (HDM2), an important oncoprotein controlling cell growth and survival, by an unknown mechanism. Here we demonstrate that AP-4 binds to an E-box located in the HDM2-P2 promoter and represses HDM2 transcrip… Show more

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Cited by 21 publications
(21 citation statements)
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“…AP4 was identified earlier to be a potent repressor in p21 regulation where it binds to E-box elements located in the proximal promoter [50], [51]. Interestingly, using unbiased proteomics approaches, Ku et al identified APE1 to be one of the potential AP4-interacting proteins bound to the E-box sequence in the HDM2 promoter [52]. We then tested the possibility that APE1 could act as AP4’s co-repressor for p21 via stable association on the p21 promoter.…”
Section: Resultsmentioning
confidence: 99%
“…AP4 was identified earlier to be a potent repressor in p21 regulation where it binds to E-box elements located in the proximal promoter [50], [51]. Interestingly, using unbiased proteomics approaches, Ku et al identified APE1 to be one of the potential AP4-interacting proteins bound to the E-box sequence in the HDM2 promoter [52]. We then tested the possibility that APE1 could act as AP4’s co-repressor for p21 via stable association on the p21 promoter.…”
Section: Resultsmentioning
confidence: 99%
“…Plasmid encoding the Tet-On 3G transactivator gene was first transfected into MDA-MB-231 cells by the Xfect TM reagent from Clontech (California, USA), as described [28]. Full-length AP4 (AP4) was constructed by cloning the PCR amplicon of the AP4 gene (using the AP4-specific primers 1 and 2 and the plasmid pcDNA3.1(-)/AP4 as a template [21] ) into the MluI site (underlined) in the pTRE3G-ZsGreen1 plasmid (Clontech).…”
Section: Double Stable Transfected Cell Line Constructionmentioning
confidence: 99%
“…breast cancer patients, and it also promotes the chemosensitivity of breast cancer cells [20]. Studies have also shown that AP4 may be involved in cell migration or EMT because AP4 is able to regulate the expression of MMP-9 (matrix metalloproteinase-9), which is involved in the breakdown of the extracellular matrix [21] and IGFBP-2 [22], which leads to a decrease in cell adhesion. More recently, AP4 was shown to be a mediator of EMT in colorectal cancer by gene expression analysis [23].…”
Section: Introductionmentioning
confidence: 97%
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“…29 Moreover, we found that sensitive SCC cells express a dramatically greater ratio of p-ΔNp63α over non-p-ΔNp63α than resistant SCC cells. 30 Using the combined DNA pull-down/iTRAQ (isobaric tag for relative and absolute quantitation) approach allowing the global analysis of transcription factors and chromatin accessory proteins bound to specific promoter, 31 we defined the critical components necessary to induce or repress ΔNp63αdependent gene expression in SCC cells upon cisplatin exposure.…”
Section: Phospho-δnp63α/microrna Feedback Regulation In Squamous Carcmentioning
confidence: 99%