Complementation between mutants of Phycomyces with abnormal phototropism

Ootaki, Tamotsu; Fischer, Eckhard; Lockhart, Paul J.

doi:10.1007/bf00267963

Cited by 63 publications

(21 citation statements)

References 9 publications

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“…Seventeen mad mutants of mating type ( -) were later used to obtain seven complementation groups of mad A through G (OOTAKI et al 1974) and 12 alleles were used to group the same seven groups with mating type (+) (OOTAKI et al 1977). More than half of these mutants are in the mad C group.…”

Section: Phycomycesmentioning

confidence: 99%

Photocontrol of Fungal Development

Gressel¹,

Rau²

1983

Photomorphogenesis

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Section: Phycomycesmentioning

confidence: 99%

Photocontrol of Fungal Development

Gressel¹,

Rau²

1983

Photomorphogenesis

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“…Upon illumination with blue light, the mycelium responds by increased synthesis of p-carotene and increased initiation of sporangiophores (5,11,13,17,18). Extensive genetic analyses have shown that at least eight complementation groups (madA through madH) are involved in the action network that controls the sensory responses of P. blakesleeanus (5,10,16,24). Recently, a transformation system for Phycomyces spp., based on the expression of the transposon Tn903-derived kanamycin resistance gene, has been established (26).…”

mentioning

confidence: 99%

Phycomyces blakesleeanus TRP1 gene: organization and functional complementation in Escherichia coli and Saccharomyces cerevisiae.

Revuelta¹,

Jayaram²

1987

Mol. Cell. Biol.

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We have cloned the gene encoding the TRPF and TRPC functions of Phycomyces blakesleeanus by complementation of the corresponding activities of Escherichia coli. TRPF also complemented a trpl mutation in Saccharomyces cerevisiae. As in other filamentous fungi, such as Neurospora and Aspergillus spp., the P. blakesleeanus TRPF and TRPC formed part of a trifunctional polypeptide encoded by a single gene (called TRP1). Transcription of TRP1 in P. blakesleeanus did not appear to be regulated by light or by the nutritional status of the culture. The information on the structure and organization of a P. blakesleeanus gene derived from these studies should be useful in devising molecular genetic strategies to analyze the sensory physiology of this organism.

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“…The genetic approach has been particularly valuable for the study of chemotaxis in bacteria (8,13), but its value has also been evident in eukaryotic sensory systems. In Phycomyces, a tentative transduction sequence or pathway has been suggested for phototropism and carotenogenesis based on a set of mutants (10). In addition, evidence has now been accumulated that phototropism by Phycomyces involves multiple photoreceptor pigments (4-6).…”

mentioning

confidence: 99%

Mutants of Arabidopsis thaliana with Decreased Amplitude in Their Phototropic Response

Khurana¹,

Ren²,

Steinitz³

et al. 1989

Plant Physiol.

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Two mutants of Arabidopsis thaliana have been identified with decreased phototropism to 450-nanometer light. Fluence-response relationships for these strains (ZR8 and ZR19) to single and multiple flashes of light show thresholds, curve shapes, and fluence for maximum curvature in 'first positive' phototropism which are the same as those of the wild type. Similarly, there is no alteration from the wild type in the kinetics of curvature or in the optimum dark period separating sequential flashes in a multiple flash regimen. In addition, in both strains, gravitropism is decreased compared to the wild type by an amount which is comparable to the decrease in phototropism. Based on reciprocal backcrosses, it appears that the alteration is due to a recessive nuclear mutation. It is suggested that ZR8 and ZR19 represent alterations in some step analogous to an amplifier, downstream of the photoreceptor pigment, and common to both phototropism and gravitropism.Although considerable effort has been invested over the past century in the study of phototropism in plants, we still do not have an understanding at the molecular level of any step in the sensory transduction pathway leading from light reception to curvature. Phototropism has been a particularly difficult process to study because the only step which can be directly observed is the final curvature. Other changes have been suggested to participate in phototropism, but their involvement is, in the final analysis, correlative. For example, it has been suggested that the spectrophotometrically observable, blue light-induced absorbance changes are mediated by the photoreceptor pigment for phototropism, and can therefore be used to assay for that pigment (1, 9, 12). However, the evidence to support this suggestion is that the spectral

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Complementation between mutants of Phycomyces with abnormal phototropism

Cited by 63 publications

References 9 publications

Photocontrol of Fungal Development

Photocontrol of Fungal Development

Phycomyces blakesleeanus TRP1 gene: organization and functional complementation in Escherichia coli and Saccharomyces cerevisiae.

Mutants of Arabidopsis thaliana with Decreased Amplitude in Their Phototropic Response

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