1995
DOI: 10.1073/pnas.92.3.890
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Complementation of the ionizing radiation sensitivity, DNA end binding, and V(D)J recombination defects of double-strand break repair mutants by the p86 Ku autoantigen.

Abstract: Two ionizing radiation-sensitive (IRS) and DNA double-strand break (DSB) mutants, sxi-3 and sxi-2, were shown to be severely deficient in a DNA end binding activity, similar to a previously described activity of the Ku autoantigen, correlating with thexrs (XRCC5) mutations. Cell fusions with xrs-6, another IRS, DSB repair-deficient cell line, defined these sxi mutants in the XRCC5 group. sr-3 cells have low expression levels of the p86Ku mRNA. Here we show the sxi-3 mutant of DSB repair is defective for both p… Show more

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Cited by 124 publications
(68 citation statements)
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“…A series of genetic and biochemical data have converged to establish the crucial role of DNA-PK in DSB repair as well as in V(D)J recombination of immunoglobulin and T-cell receptor genes (reviewed in references 35, 69, and 75). Defective DSB repair and V(D)J recombination in X-raysensitive rodent cell lines of the complementation group for XRCC5 were rescued by transfection of the human Ku86 cDNA (9,57,59), and mutations that are responsible for the X-ray sensitivity of these cells were mapped to the Ku86 gene, demonstrating unambiguously that Ku86 is the product of XRCC5 (25). An additional role for DNA-PK in DNA replication has been suggested by its ability to phosphorylate replication protein A (10,52).…”
mentioning
confidence: 81%
“…A series of genetic and biochemical data have converged to establish the crucial role of DNA-PK in DSB repair as well as in V(D)J recombination of immunoglobulin and T-cell receptor genes (reviewed in references 35, 69, and 75). Defective DSB repair and V(D)J recombination in X-raysensitive rodent cell lines of the complementation group for XRCC5 were rescued by transfection of the human Ku86 cDNA (9,57,59), and mutations that are responsible for the X-ray sensitivity of these cells were mapped to the Ku86 gene, demonstrating unambiguously that Ku86 is the product of XRCC5 (25). An additional role for DNA-PK in DNA replication has been suggested by its ability to phosphorylate replication protein A (10,52).…”
mentioning
confidence: 81%
“…Now these mutant CHO cells are known to be deficient in Ku80 (Boubnov et al, 1995;Getts and Stamato, 1994;Rathmell and Chu, 1994). Ku is a heterodimeric protein, containing two subunits, Ku80 and Ku70, both of which bind with high affinity to double-strand DNA (dsDNA) ends.…”
Section: Introductionmentioning
confidence: 99%
“…In addition, group 5 cells lack a DNA-end-binding (DEB) activity with an abundance, nuclear localization, DNA substrate specificities, and antigenic determinants similar to those of Ku (13,36,37). Transfection of the human Ku86 cDNA into group 5 cells partially restores DEB activity, X-ray resistance, and V(D)J recombination activity in different mutants belonging to this group (3,42,44).…”
mentioning
confidence: 99%