Complementation Plasmids, Inducible Gene-Expression Systems, and Reporters for Staphylococci

Abstract

A cornucopia of methods and molecular tools is available for genetic modification of staphylococci, as shown for at least ten different species to date (Prax et al. Microbiology 159:421-435, 2013). This chapter reviews a number of frequently used vectors for complementation purposes that usually replicate in E. coli and staphylococci and differ in parameters including copy number, mode of replication, and sequence length. Systems for the artificial control of gene expression are described that are modulated by low-molecular-weight effectors such as metal cations, carbohydrates, and antibiotics. Finally, the usefulness of reporter proteins that exhibit enzymatic or autofluorescent characteristics in staphylococci is highlighted.

Anti-Staphylococcus aureus potential of compounds from Ganoderma sp.: A comprehensive molecular docking and simulation approaches

“…Amongst the Staphylococcus species, Staphylococcus aureus stands out due to its prevalence and ability to cause severe, potentially fatal conditions such as osteomyelitis, endocarditis, sepsis, meningitis, skin syndrome with scabs, toxic shock syndrome, and food poisoning [ 2 , 4 , 5 ]. S. aureus typically colonizes the skin and nasopharynx, posing a threat primarily to immunocompromised individuals [ 6 ]. Alarmingly, approximately 50% of current S. aureus infections are resistant to multiple antibiotics, including penicillin, methicillin, tetracycline, ampicillin, and erythromycin [ 1 , 3 ].…”

Integrating in Silico and In Vitro Studies to Screen Anti-Staphylococcus aureus Activity From Vietnamese Ganoderma multiplicatum and Ganoderma sinense

“…The TIP3P explicit solvation model was used. 35 The simulation box dimensions were 76 Å, 76 Å, and 76 Å in x, y, and z. The parameters for the top docking results were generated using CHARMM general force field.…”

“…2,4,5 This species usually parasitizes the skin and nasopharynx, causing disease in immunodeficient people. 6 Approximately 50% of current S. aureus infections are resistant to multiple antibiotics, including penicillin, methicillin, tetracycline, ampicillin, and erythromycin. 1,3 The use of multiple antibiotics has promoted the development of various resistant S. aureus strains, including to linezolid, quinupristin, and dalfopristin.…”