Complete base sequence for the mitochondrial large subunit ribosomal RNA of the gypsy moth Lymantha dispar(L.)

Abstract

A 1355 bp sequence (accession number L32141) isolated from a gypsy moth (Lymantria dispar) cDNA library showed 68-74% sequence identity to mitochondrial large subunit ribosomal RNA (mt IrRNA) sequences of Locusta migratoria, Apis mellifera, Aedes albopictus, Anopheles gambiae and two Drosophila species. A comparison of the primary sequences of the mt IrRNAs from the above insects in four orders and from Esherichia coli demonstrated regions of conservation which presumably correspond to regions of functional and/or structural homology. A secondary structure for the gypsy moth mt IrRNA sequence was derived based on the proposed secondary structures of Drosophila yakuba and Aedes albopictus mt IrRNAs (Gutell & Fox, Nucleic Acid Res 16 (Suppl.), r175-r269, 1988). This sequence was found to hybridize to about 10-15% of the clones in several (eleven) gypsy moth cDNA libraries.

Secondary structure and conserved motifs of the frequently sequenced domains IV and V of the insect mitochondrial large subunit rRNA gene

“…In all of the termites, except Mastotermes darwiniensis and Incistitermes snyderi , helix 67 can be drawn as a 4 bp structure. Davis et al . (1994) includes the preceding nucleotides from the region between 66′ and 67, and 67′ and 64′ as part of helix 67.…”

“…The Gutell & Fox (1988) Drosophila melanogaster mit LSU model has since been updated by R. R. Gutell et al . (unpublished) and thus the Davis et al . (1994) Lymantria mit LSU model requires re‐examination.…”

“…Other hydrogen bonds adjacent to this interaction are broken in some of the termites; however, compensatory substitutions among leafhoppers and Rhagoletis flies provide support for their existence. Davis et al (1994) pair part of the asymmetric bulge in Lymantria. This arrangement is not supported by compensatory substitutions in other insect taxa.…”

“…Davis et al . (1994) published the complete mit LSU secondary structure of the gypsy moth Lymantria dispar based on the D. yakuba and Aedes albopticus secondary structure models from Gutell & Fox (1988).…”

Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes ofApis mellifera(Insecta: Hymenoptera): structure, organization, and retrotransposable elements

“…Since the earliest structural predictions for arthropod 16S rRNA, namely those from Drosophila yakuba (Clary & Wolstenholme, 1985), Locusta spp. (Uhlenbusch et al ., 1987), D. melanogaster (Gutell & Fox, 1988) and gypsy moth, Lymantria dispar (Davis et al ., 1994), more refined models have been proposed based on larger comparisons across divergent taxa, e.g. Deltocephalus ‐like leafhoppers (Fang et al ., 1993), families of Hymenoptera (Dowton & Austin, 1994), families of Dermaptera (Kambhampati et al ., 1996), families of Orthoptera (Flook & Rowell, 1997) and groups of spiders (Smith & Bond, 2003).…”